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Matches in SemOpenAlex for { <https://semopenalex.org/work/W4386003509> ?p ?o ?g. }

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• W4386003509 abstract "We present a novel dual-mode DNA-walker based optical fiber biosensor (DMDW-Opt biosensor) for sensitive assay of micro-RNAs in bio-samples. In the sensor system, we develop a new strategy for the cascade amplification, DNA-walker/rolling cycle amplification (RCA), by the use of the residue track of the walker. The strategy can significantly improve the response of the sensor and avoid any tedious operation procedure. Dual-mode readouts, i.e., fluorescence and chemiluminescence, are measured independently without interfering with each other to achieve reliable and accurate analysis. Optical fibers with the surface modified by gold nanoparticles are utilized as the support for fabrication of the sensor, which would be benefit for developing miniaturized and portable sensing devices. The performance of the proposed method is evaluated by using micro-RNAs (MiR-155 and MiR-21) as the analytical target. The method is successfully applied for accurate determination of micoRNAs in human serum and MCF-7 cells. Our method can perform sensitive assays of MiR-155 with limit-of-detection as low as 97.72 fM and 11.22 fM, MiR-21 with limit-of-detection as low as 107.15 fM and 8.32 fM for the fluorescence- and the chemiluminescence-readout respectively, and the biosensor exhibits excellent specificity, reproducibility and storage stability, indicating its valuable potential applications for sensing trace-amount targets in complicated real samples." @default.
• W4386003509 created "2023-08-20" @default.
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• W4386003509 date "2023-11-01" @default.
• W4386003509 modified "2023-10-12" @default.
• W4386003509 title "Dual-mode DNA walker-based optical fiber biosensor for ultrasensitive detection of microRNAs" @default.
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• W4386003509 doi "https://doi.org/10.1016/j.bios.2023.115613" @default.
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