Matches in SemOpenAlex for { <https://semopenalex.org/work/W4386003885> ?p ?o ?g. }
- W4386003885 endingPage "106310" @default.
- W4386003885 startingPage "106310" @default.
- W4386003885 abstract "Brucella abortus is facultative intracellular pathogen that causes chronic persistent infections and results in abortion and infertility in food animals. Recurrent infections can be one of the results of persister cells formation that transiently displays phenotypic tolerance to high dose of antibiotics treatment. We examined persister cells formation of B. abortus strain A19 in stationary phase and investigated a potential role for the (p)ppGpp synthetase Rsh in this process. We found that B. abortus stationary phase cells can produce higher levels of multi-drugs tolerant persister cells in vitro under high dose of antibiotics (20 × MIC) exposure than do exponential phase cells. Persister cell formation was also induced with environmental stressors pH 4.5, 0.01 M PBS (pH7.0), 2% NaCl and 25 °C, upon exposure to ampicillin, enrofloxacin and rifampicin. Persister cells were not formed following exposure to 1 mM H2O2. The numbers of persister cells were significantly increased following uptake of B. abortus stationary phase cells by RAW264.7 macrophages in contrast with cultures in TSB liquid medium. Environmental stressors to B. abortus significantly increased expression of rsh mRNA level. The rsh null mutant (Δrsh) formed significantly fewer persister cells than the complemented (CΔrsh) and wildtype (WT) strains under high dose of rifampicin in vitro. These data for the first time demonstrate that B. abortus can produce multi-drug tolerant persister cells in stationary phase. The (p)ppGpp synthetase Rsh is necessary for persister cell formation in B. abortus in the presence of rifampicin. On this basis, a new understanding of the recurrent infections of Brucella was advanced, thus provided a new basis for revelation of pathogenic mechanism of the chronic persistent infection in Brucella." @default.
- W4386003885 created "2023-08-20" @default.
- W4386003885 creator A5006501065 @default.
- W4386003885 creator A5007006941 @default.
- W4386003885 creator A5010325699 @default.
- W4386003885 creator A5021119255 @default.
- W4386003885 creator A5021345008 @default.
- W4386003885 creator A5026231812 @default.
- W4386003885 creator A5043894450 @default.
- W4386003885 creator A5046878062 @default.
- W4386003885 creator A5071037763 @default.
- W4386003885 creator A5073933722 @default.
- W4386003885 creator A5075785492 @default.
- W4386003885 creator A5082255460 @default.
- W4386003885 creator A5091614309 @default.
- W4386003885 date "2023-10-01" @default.
- W4386003885 modified "2023-10-07" @default.
- W4386003885 title "(P)ppGpp synthetase Rsh participates in rifampicin tolerance of persister cells in Brucella abortus in vitro" @default.
- W4386003885 cites W1243735121 @default.
- W4386003885 cites W1934154653 @default.
- W4386003885 cites W1974053222 @default.
- W4386003885 cites W1995093021 @default.
- W4386003885 cites W2022664428 @default.
- W4386003885 cites W2026397514 @default.
- W4386003885 cites W2089184777 @default.
- W4386003885 cites W2099195467 @default.
- W4386003885 cites W2101366952 @default.
- W4386003885 cites W2107517822 @default.
- W4386003885 cites W2110926912 @default.
- W4386003885 cites W2118904810 @default.
- W4386003885 cites W2141883227 @default.
- W4386003885 cites W2148323109 @default.
- W4386003885 cites W2336504120 @default.
- W4386003885 cites W2404360111 @default.
- W4386003885 cites W2586386056 @default.
- W4386003885 cites W2620525720 @default.
- W4386003885 cites W2775828058 @default.
- W4386003885 cites W2800885808 @default.
- W4386003885 cites W2810844496 @default.
- W4386003885 cites W2886565498 @default.
- W4386003885 cites W2902168927 @default.
- W4386003885 cites W2921806482 @default.
- W4386003885 cites W2925280433 @default.
- W4386003885 cites W2940382580 @default.
- W4386003885 cites W2953384242 @default.
- W4386003885 cites W2958530108 @default.
- W4386003885 cites W2958590276 @default.
- W4386003885 cites W2969426479 @default.
- W4386003885 cites W2971702659 @default.
- W4386003885 cites W2984647559 @default.
- W4386003885 cites W2999084525 @default.
- W4386003885 cites W3008038827 @default.
- W4386003885 cites W3014630988 @default.
- W4386003885 cites W3023088732 @default.
- W4386003885 cites W3027221637 @default.
- W4386003885 cites W3043272814 @default.
- W4386003885 cites W3045384390 @default.
- W4386003885 cites W3083008593 @default.
- W4386003885 cites W3089561862 @default.
- W4386003885 cites W3096180043 @default.
- W4386003885 cites W3105705776 @default.
- W4386003885 cites W3135488369 @default.
- W4386003885 cites W3207324324 @default.
- W4386003885 cites W4211006881 @default.
- W4386003885 cites W4213440132 @default.
- W4386003885 cites W4220852783 @default.
- W4386003885 cites W4220968640 @default.
- W4386003885 cites W4223893169 @default.
- W4386003885 cites W4243788202 @default.
- W4386003885 cites W4288038147 @default.
- W4386003885 cites W4307499026 @default.
- W4386003885 cites W4309519438 @default.
- W4386003885 doi "https://doi.org/10.1016/j.micpath.2023.106310" @default.
- W4386003885 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/37604214" @default.
- W4386003885 hasPublicationYear "2023" @default.
- W4386003885 type Work @default.
- W4386003885 citedByCount "0" @default.
- W4386003885 crossrefType "journal-article" @default.
- W4386003885 hasAuthorship W4386003885A5006501065 @default.
- W4386003885 hasAuthorship W4386003885A5007006941 @default.
- W4386003885 hasAuthorship W4386003885A5010325699 @default.
- W4386003885 hasAuthorship W4386003885A5021119255 @default.
- W4386003885 hasAuthorship W4386003885A5021345008 @default.
- W4386003885 hasAuthorship W4386003885A5026231812 @default.
- W4386003885 hasAuthorship W4386003885A5043894450 @default.
- W4386003885 hasAuthorship W4386003885A5046878062 @default.
- W4386003885 hasAuthorship W4386003885A5071037763 @default.
- W4386003885 hasAuthorship W4386003885A5073933722 @default.
- W4386003885 hasAuthorship W4386003885A5075785492 @default.
- W4386003885 hasAuthorship W4386003885A5082255460 @default.
- W4386003885 hasAuthorship W4386003885A5091614309 @default.
- W4386003885 hasConcept C136622501 @default.
- W4386003885 hasConcept C159047783 @default.
- W4386003885 hasConcept C202751555 @default.
- W4386003885 hasConcept C2778607973 @default.
- W4386003885 hasConcept C2780767784 @default.
- W4386003885 hasConcept C2781429187 @default.
- W4386003885 hasConcept C501593827 @default.