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- W4386019898 abstract "Abstract Prophages control their lifestyle to either be maintained within the host genome or enter the lytic cycle. Bacillus subtilis contains the SPβ prophage whose lysogenic state depends on the MrpR (YopR) protein, a key component of the lysis-lysogeny decision system. Using a historic B. subtilis strain harboring the heat-sensitive SPβ c2 mutant, we demonstrate that the lytic cycle of SPβ c2 can be induced by heat due to a single nucleotide exchange in the mrpR gene, rendering the encoded MrpRG136E protein temperature-sensitive. Structural characterization revealed that MrpR is a DNA-binding protein resembling the overall fold of tyrosine recombinases. MrpR has lost its recombinase function and the G136E exchange impairs its higher-order structure and DNA binding activity. Genome-wide profiling of MrpR binding revealed its association with the previously identified SPbeta repeated element (SPBRE) in the SPβ genome. MrpR functions as a master repressor of SPβ that binds to this conserved element to maintain lysogeny. The heat-inducible excision of the SPβ c2 mutant remains reliant on the serine recombinase SprA. A suppressor mutant analysis identified a previously unknown component of the lysis-lysogeny management system that is crucial for the induction of the lytic cycle of SPβ." @default.
- W4386019898 created "2023-08-22" @default.
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- W4386019898 creator A5087631732 @default.
- W4386019898 date "2023-08-21" @default.
- W4386019898 modified "2023-10-15" @default.
- W4386019898 title "Structural and functional characterization of MrpR, the master repressor of the <i>Bacillus subtilis</i> prophage SPβ" @default.
- W4386019898 cites W1531868556 @default.
- W4386019898 cites W1534102217 @default.
- W4386019898 cites W1550820303 @default.
- W4386019898 cites W1590546489 @default.
- W4386019898 cites W1716657688 @default.
- W4386019898 cites W1964777192 @default.
- W4386019898 cites W1974184850 @default.
- W4386019898 cites W1976023732 @default.
- W4386019898 cites W1979136121 @default.
- W4386019898 cites W1981536086 @default.
- W4386019898 cites W2000106654 @default.
- W4386019898 cites W2003424506 @default.
- W4386019898 cites W2004996851 @default.
- W4386019898 cites W2009005740 @default.
- W4386019898 cites W2010379434 @default.
- W4386019898 cites W2019304549 @default.
- W4386019898 cites W2036650062 @default.
- W4386019898 cites W2038663142 @default.
- W4386019898 cites W2039972425 @default.
- W4386019898 cites W2047796158 @default.
- W4386019898 cites W2047984143 @default.
- W4386019898 cites W2052826095 @default.
- W4386019898 cites W2060445972 @default.
- W4386019898 cites W2062381269 @default.
- W4386019898 cites W2071955064 @default.
- W4386019898 cites W2072152013 @default.
- W4386019898 cites W2080357982 @default.
- W4386019898 cites W2093263361 @default.
- W4386019898 cites W2094626148 @default.
- W4386019898 cites W2100303812 @default.
- W4386019898 cites W2103722640 @default.
- W4386019898 cites W2105128860 @default.
- W4386019898 cites W2115029043 @default.
- W4386019898 cites W2116553535 @default.
- W4386019898 cites W2116582554 @default.
- W4386019898 cites W2124703740 @default.
- W4386019898 cites W2126705194 @default.
- W4386019898 cites W2134201060 @default.
- W4386019898 cites W2135267933 @default.
- W4386019898 cites W2144081223 @default.
- W4386019898 cites W2147874841 @default.
- W4386019898 cites W2149516046 @default.
- W4386019898 cites W2151788366 @default.
- W4386019898 cites W2152633782 @default.
- W4386019898 cites W2157009395 @default.
- W4386019898 cites W2170551349 @default.
- W4386019898 cites W2174377965 @default.
- W4386019898 cites W2282977467 @default.
- W4386019898 cites W2290768068 @default.
- W4386019898 cites W2316770024 @default.
- W4386019898 cites W2464464964 @default.
- W4386019898 cites W2472136907 @default.
- W4386019898 cites W2498840197 @default.
- W4386019898 cites W2567114929 @default.
- W4386019898 cites W2579294549 @default.
- W4386019898 cites W2586340362 @default.
- W4386019898 cites W2606257019 @default.
- W4386019898 cites W2618758136 @default.
- W4386019898 cites W2802851075 @default.
- W4386019898 cites W2890546854 @default.
- W4386019898 cites W2894693615 @default.
- W4386019898 cites W2900464177 @default.
- W4386019898 cites W2902850225 @default.
- W4386019898 cites W2906071039 @default.
- W4386019898 cites W2912817179 @default.
- W4386019898 cites W2946832795 @default.
- W4386019898 cites W2952597168 @default.
- W4386019898 cites W2955168413 @default.
- W4386019898 cites W2973457155 @default.
- W4386019898 cites W2995522508 @default.
- W4386019898 cites W3003557640 @default.
- W4386019898 cites W3031705300 @default.
- W4386019898 cites W3033568498 @default.
- W4386019898 cites W3039607457 @default.
- W4386019898 cites W3047201784 @default.
- W4386019898 cites W3110170914 @default.
- W4386019898 cites W3167926034 @default.
- W4386019898 cites W3175194842 @default.
- W4386019898 cites W3177828909 @default.
- W4386019898 cites W3197755366 @default.
- W4386019898 cites W3204904100 @default.
- W4386019898 cites W4211242168 @default.
- W4386019898 cites W4221035669 @default.