Matches in SemOpenAlex for { <https://semopenalex.org/work/W4386048149> ?p ?o ?g. }
- W4386048149 abstract "Abstract Environmentally friendly industrial and biotech processes greatly benefit from enzyme‐based technologies. Their use is often possible only when the enzyme‐catalytic mechanism is thoroughly known. Thus, atomic‐level knowledge of a Michaelis enzyme‐substrate complex, revealing molecular details of substrate recognition and catalytic chemistry, is crucial for understanding and then rationally extending or improving enzyme‐catalyzed reactions. However, many known enzymes sample huge protein conformational space, often preventing complete structural characterization by X‐ray crystallography. Moreover, using a cognate substrate is problematic since its conversion into a reaction product in the presence of the enzyme will prevent the capture of the enzyme‐substrate conformation in an activated state. Here, we outlined how to deal with such obstacles, focusing on the recent discovery of a Renilla ‐type bioluminescence reaction mechanism facilitated by a combination of engineered ancestral enzyme and the availability of a non‐oxidizable luciferin analogue. The automated ancestral sequence reconstructions using FireProt ASR provided a thermostable enzyme suited for structural studies, and a stable luciferin analogue azacoelenterazine provided a structurally cognate chemical incapable of catalyzed oxidation. We suggest that an analogous strategy can be applied to various enzymes with unknown catalytic mechanisms and poor crystallizability." @default.
- W4386048149 created "2023-08-23" @default.
- W4386048149 creator A5040585952 @default.
- W4386048149 creator A5054204354 @default.
- W4386048149 creator A5068900414 @default.
- W4386048149 creator A5087659225 @default.
- W4386048149 date "2023-09-08" @default.
- W4386048149 modified "2023-10-17" @default.
- W4386048149 title "Deciphering Enzyme Mechanisms with Engineered Ancestors and Substrate Analogues" @default.
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