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- W4386053721 abstract "Pseudomonas aeruginosa is a nosocomial bacterium responsible for variety of infections. Inappropriate use of antibiotics could lead to emergence of multidrug-resistant (MDR) P. aeruginosa strains. Herein, a virulent phage; vB_PaeM_PS3 was isolated and tested for its application as alternative to antibiotics for controlling P. aeruginosa infections.Phage morphology was observed using transmission electron microscopy (TEM). The phage host range and efficiency of plating (EOP) in addition to phage stability were analyzed. One-step growth curve was performed to detect phage growth kinetics. The impact of isolated phage on planktonic cells and biofilms was assessed. The phage genome was sequenced. Finally, the therapeutic potential of vB_PaeM_PS3 was determined in vivo.Isolated phage has an icosahedral head and a contractile tail and was assigned to the family Myoviridae. The phage vB_PaeM_PS3 displayed a broad host range, strong bacteriolytic ability, and higher environmental stability. Isolated phage showed a short latent period and large burst size. Importantly, the phage vB_PaeM_PS3 effectively eradicated bacterial biofilms. The genome of vB_PaeM_PS3 consists of 93,922 bp of dsDNA with 49.39% G + C content. It contains 171 predicted open reading frames (ORFs) and 14 genes as tRNA. Interestingly, the phage vB_PaeM_PS3 significantly attenuated P. aeruginosa virulence in host where the survival of bacteria-infected mice was markedly enhanced following phage treatment. Moreover, the colonizing capability of P. aeruginosa was markedly impaired in phage-treated mice as compared to untreated infected mice.Based on these findings, isolated phage vB_PaeM_PS3 could be potentially considered for treating of P. aeruginosa infections." @default.
- W4386053721 created "2023-08-23" @default.
- W4386053721 creator A5012663317 @default.
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- W4386053721 creator A5051467292 @default.
- W4386053721 creator A5082726037 @default.
- W4386053721 date "2023-08-23" @default.
- W4386053721 modified "2023-10-17" @default.
- W4386053721 title "A novel lytic phage exhibiting a remarkable in vivo therapeutic potential and higher antibiofilm activity against Pseudomonas aeruginosa" @default.
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- W4386053721 cites W1988967448 @default.
- W4386053721 cites W1992128867 @default.
- W4386053721 cites W1992551495 @default.
- W4386053721 cites W1997824495 @default.
- W4386053721 cites W2004223516 @default.
- W4386053721 cites W2014601460 @default.
- W4386053721 cites W2040274090 @default.
- W4386053721 cites W2040416009 @default.
- W4386053721 cites W2040515264 @default.
- W4386053721 cites W2043346930 @default.
- W4386053721 cites W2045860509 @default.
- W4386053721 cites W2051945273 @default.
- W4386053721 cites W2055043387 @default.
- W4386053721 cites W2061878956 @default.
- W4386053721 cites W2063089818 @default.
- W4386053721 cites W2068345774 @default.
- W4386053721 cites W2069362245 @default.
- W4386053721 cites W2071605567 @default.
- W4386053721 cites W2078117130 @default.
- W4386053721 cites W2087996087 @default.
- W4386053721 cites W2089015524 @default.
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- W4386053721 cites W2101757664 @default.
- W4386053721 cites W2105574710 @default.
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- W4386053721 cites W2163204395 @default.
- W4386053721 cites W2165891191 @default.
- W4386053721 cites W2171452890 @default.
- W4386053721 cites W2315755647 @default.
- W4386053721 cites W2419630738 @default.
- W4386053721 cites W2474583225 @default.
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- W4386053721 cites W2895726097 @default.
- W4386053721 cites W2903295354 @default.
- W4386053721 cites W2903381708 @default.
- W4386053721 cites W2918903869 @default.
- W4386053721 cites W2941342985 @default.
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- W4386053721 cites W3124792643 @default.
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- W4386053721 doi "https://doi.org/10.1007/s10096-023-04649-y" @default.
- W4386053721 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/37608144" @default.
- W4386053721 hasPublicationYear "2023" @default.
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