FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4386055615> ?p ?o ?g. }

• W4386055615 abstract "Summary Tardigrades are known for their ability to survive remarkable extremes, but relatively little is known about the molecular underpinnings that allow for their extraordinary stress tolerance. We devised an expression cloning approach to screen for novel protectants from desiccation-tolerant tardigrades. We expressed cDNA libraries from the tardigrades Hypsibius exemplaris and Ramazzottius varieornatus in E. coli and subjected the bacteria to desiccation. Sequencing the populations of surviving bacteria revealed enrichment of mitochondrial single-stranded DNA-binding proteins (mtSSBs) from both tardigrade species. Expression of mtSSBs in bacteria improved desiccation survival to similar levels as some of the best protectants known to date. The DNA-binding activity of the oligonucleotide/oligosaccharide binding (OB) fold domain of mtSSB was necessary and sufficient to improve desiccation tolerance. By comparing the efficacy of mtSSBs from multiple organisms, we provide evidence that desicco-protection is a conserved feature of mtSSBs. More broadly, any OB-fold containing protein may act similarly to promote desiccation tolerance, likely by coating ssDNA to limit damage during desiccation. These results identify single-stranded DNA-binding activity as a potent contributor to extreme desiccation tolerance." @default.
• W4386055615 created "2023-08-23" @default.
• W4386055615 creator A5001148098 @default.
• W4386055615 creator A5008285624 @default.
• W4386055615 creator A5068057210 @default.
• W4386055615 creator A5090874399 @default.
• W4386055615 date "2023-08-22" @default.
• W4386055615 modified "2023-10-10" @default.
• W4386055615 title "A bacterial expression cloning screen reveals tardigrade single-stranded DNA-binding proteins as potent desicco-protectants" @default.
• W4386055615 cites W1526102850 @default.
• W4386055615 cites W1577451703 @default.
• W4386055615 cites W1658672685 @default.
• W4386055615 cites W1806123628 @default.
• W4386055615 cites W1967448179 @default.
• W4386055615 cites W1989108993 @default.
• W4386055615 cites W1989345028 @default.
• W4386055615 cites W1989840530 @default.
• W4386055615 cites W1993140443 @default.
• W4386055615 cites W2003069003 @default.
• W4386055615 cites W2016572584 @default.
• W4386055615 cites W2021341670 @default.
• W4386055615 cites W2044260971 @default.
• W4386055615 cites W2060788948 @default.
• W4386055615 cites W2073232799 @default.
• W4386055615 cites W2106102828 @default.
• W4386055615 cites W2116541356 @default.
• W4386055615 cites W2120280317 @default.
• W4386055615 cites W2125980191 @default.
• W4386055615 cites W2145301542 @default.
• W4386055615 cites W2153436893 @default.
• W4386055615 cites W2167521745 @default.
• W4386055615 cites W2179438025 @default.
• W4386055615 cites W2523589472 @default.
• W4386055615 cites W2596822322 @default.
• W4386055615 cites W2606623351 @default.
• W4386055615 cites W2799524357 @default.
• W4386055615 cites W2809043773 @default.
• W4386055615 cites W2810450454 @default.
• W4386055615 cites W2901607021 @default.
• W4386055615 cites W2914039839 @default.
• W4386055615 cites W2977368063 @default.
• W4386055615 cites W2979014048 @default.
• W4386055615 cites W2991012415 @default.
• W4386055615 cites W2999168229 @default.
• W4386055615 cites W3000766842 @default.
• W4386055615 cites W3022933121 @default.
• W4386055615 cites W3087754010 @default.
• W4386055615 cites W3091024877 @default.
• W4386055615 cites W3093856688 @default.
• W4386055615 cites W3099436785 @default.
• W4386055615 cites W3121927349 @default.
• W4386055615 cites W3156288747 @default.
• W4386055615 cites W3177828909 @default.
• W4386055615 cites W4200124957 @default.
• W4386055615 cites W4220746620 @default.
• W4386055615 cites W4223442517 @default.
• W4386055615 cites W4231685787 @default.
• W4386055615 cites W4322487406 @default.
• W4386055615 cites W4367297747 @default.
• W4386055615 cites W4367835393 @default.
• W4386055615 cites W4378715905 @default.
• W4386055615 doi "https://doi.org/10.1101/2023.08.21.554171" @default.
• W4386055615 hasPublicationYear "2023" @default.
• W4386055615 type Work @default.
• W4386055615 citedByCount "0" @default.
• W4386055615 crossrefType "posted-content" @default.
• W4386055615 hasAuthorship W4386055615A5001148098 @default.
• W4386055615 hasAuthorship W4386055615A5008285624 @default.
• W4386055615 hasAuthorship W4386055615A5068057210 @default.
• W4386055615 hasAuthorship W4386055615A5090874399 @default.
• W4386055615 hasBestOaLocation W43860556151 @default.
• W4386055615 hasConcept C104317684 @default.
• W4386055615 hasConcept C121050878 @default.
• W4386055615 hasConcept C143425029 @default.
• W4386055615 hasConcept C174332187 @default.
• W4386055615 hasConcept C187882448 @default.
• W4386055615 hasConcept C199360897 @default.
• W4386055615 hasConcept C2777895711 @default.
• W4386055615 hasConcept C34920940 @default.
• W4386055615 hasConcept C41008148 @default.
• W4386055615 hasConcept C50402729 @default.
• W4386055615 hasConcept C523546767 @default.
• W4386055615 hasConcept C54355233 @default.
• W4386055615 hasConcept C552990157 @default.
• W4386055615 hasConcept C59822182 @default.
• W4386055615 hasConcept C86803240 @default.
• W4386055615 hasConceptScore W4386055615C104317684 @default.
• W4386055615 hasConceptScore W4386055615C121050878 @default.
• W4386055615 hasConceptScore W4386055615C143425029 @default.
• W4386055615 hasConceptScore W4386055615C174332187 @default.
• W4386055615 hasConceptScore W4386055615C187882448 @default.
• W4386055615 hasConceptScore W4386055615C199360897 @default.
• W4386055615 hasConceptScore W4386055615C2777895711 @default.
• W4386055615 hasConceptScore W4386055615C34920940 @default.
• W4386055615 hasConceptScore W4386055615C41008148 @default.
• W4386055615 hasConceptScore W4386055615C50402729 @default.
• W4386055615 hasConceptScore W4386055615C523546767 @default.
• W4386055615 hasConceptScore W4386055615C54355233 @default.
• W4386055615 hasConceptScore W4386055615C552990157 @default.
• W4386055615 hasConceptScore W4386055615C59822182 @default.

• next