Matches in SemOpenAlex for { <https://semopenalex.org/work/W4386085412> ?p ?o ?g. }
Showing items 1 to 87 of
87
with 100 items per page.
- W4386085412 endingPage "11" @default.
- W4386085412 startingPage "1" @default.
- W4386085412 abstract "Endocrine disruptors in marine environments represented by estrogens lead to reverse health phenomena. To obtain a more effective way to reflect and detect environmental estrogens pollution, a method was developed to obtain the full-length cDNA coding vitellogenin gene in B. sinensis, induced by 17β-estradiol (E2) solution. We have downloaded 16 fish gene sequences from the NCBI database and designed PCR primers accordingly. Based on the quantitative real-time PCR method (qRT-PCR), we analyze the differences in gene expression under the conditions of different E2 exposure times in the low, middle, and high-dose groups. The full-length cDNA consists of 4738 nucleotides with a reading frame encoding 1540 amino acid residues. In vitro recombinant plasmids were constructed and transferred to E. coli BL21 for vitellogenin expression. Efficient fusion expression was obtained by IPTG at 16°C, and the expressed target protein (680 amino acids, 75 kDa) existed in a soluble state, accounting for more than 25% of the total soluble protein. We prepared monoclonal antibodies using established immunohistochemistry to detect vitellogenin expression sites in sexually mature female fish. Our study shows that the expression sites of Vg in sexually mature female fish are mainly distributed in the fishtail, hepatopancreas, intestine, muscle, ovary, and pronephric kidney. In conclusion, the vitellogenin from B. sinensis could be used as a biomarker of environmental estrogens to achieve rapid detection in the marine environment and the subsequent experiments of development in colloidal gold strips after this research would be established to provide a highly efficient and convenient detection method for environment pollution." @default.
- W4386085412 created "2023-08-24" @default.
- W4386085412 creator A5013089717 @default.
- W4386085412 creator A5017254378 @default.
- W4386085412 creator A5045806340 @default.
- W4386085412 creator A5051056359 @default.
- W4386085412 creator A5072513760 @default.
- W4386085412 creator A5072994968 @default.
- W4386085412 creator A5088064576 @default.
- W4386085412 date "2023-08-23" @default.
- W4386085412 modified "2023-10-13" @default.
- W4386085412 title "Cloning, Expression Analysis, and Detection of the Vitellogenin in the Chinese Black Sleeper Bostrychus sinensis" @default.
- W4386085412 cites W1571613281 @default.
- W4386085412 cites W1706550428 @default.
- W4386085412 cites W1986459072 @default.
- W4386085412 cites W2048081365 @default.
- W4386085412 cites W2056680260 @default.
- W4386085412 cites W2058844619 @default.
- W4386085412 cites W2061779841 @default.
- W4386085412 cites W2093912414 @default.
- W4386085412 cites W2107156968 @default.
- W4386085412 cites W2148619299 @default.
- W4386085412 cites W2163630709 @default.
- W4386085412 cites W2197960752 @default.
- W4386085412 cites W2523637447 @default.
- W4386085412 cites W2747929863 @default.
- W4386085412 cites W2768079269 @default.
- W4386085412 cites W2923451077 @default.
- W4386085412 cites W2990762611 @default.
- W4386085412 cites W3126507765 @default.
- W4386085412 doi "https://doi.org/10.1155/2023/2172611" @default.
- W4386085412 hasPublicationYear "2023" @default.
- W4386085412 type Work @default.
- W4386085412 citedByCount "0" @default.
- W4386085412 crossrefType "journal-article" @default.
- W4386085412 hasAuthorship W4386085412A5013089717 @default.
- W4386085412 hasAuthorship W4386085412A5017254378 @default.
- W4386085412 hasAuthorship W4386085412A5045806340 @default.
- W4386085412 hasAuthorship W4386085412A5051056359 @default.
- W4386085412 hasAuthorship W4386085412A5072513760 @default.
- W4386085412 hasAuthorship W4386085412A5072994968 @default.
- W4386085412 hasAuthorship W4386085412A5088064576 @default.
- W4386085412 hasBestOaLocation W43860854121 @default.
- W4386085412 hasConcept C104317684 @default.
- W4386085412 hasConcept C150194340 @default.
- W4386085412 hasConcept C153911025 @default.
- W4386085412 hasConcept C167625842 @default.
- W4386085412 hasConcept C187882448 @default.
- W4386085412 hasConcept C2776367567 @default.
- W4386085412 hasConcept C2779078696 @default.
- W4386085412 hasConcept C2909208804 @default.
- W4386085412 hasConcept C47289529 @default.
- W4386085412 hasConcept C505870484 @default.
- W4386085412 hasConcept C54355233 @default.
- W4386085412 hasConcept C86803240 @default.
- W4386085412 hasConceptScore W4386085412C104317684 @default.
- W4386085412 hasConceptScore W4386085412C150194340 @default.
- W4386085412 hasConceptScore W4386085412C153911025 @default.
- W4386085412 hasConceptScore W4386085412C167625842 @default.
- W4386085412 hasConceptScore W4386085412C187882448 @default.
- W4386085412 hasConceptScore W4386085412C2776367567 @default.
- W4386085412 hasConceptScore W4386085412C2779078696 @default.
- W4386085412 hasConceptScore W4386085412C2909208804 @default.
- W4386085412 hasConceptScore W4386085412C47289529 @default.
- W4386085412 hasConceptScore W4386085412C505870484 @default.
- W4386085412 hasConceptScore W4386085412C54355233 @default.
- W4386085412 hasConceptScore W4386085412C86803240 @default.
- W4386085412 hasFunder F4320335777 @default.
- W4386085412 hasLocation W43860854121 @default.
- W4386085412 hasOpenAccess W4386085412 @default.
- W4386085412 hasPrimaryLocation W43860854121 @default.
- W4386085412 hasRelatedWork W2009966535 @default.
- W4386085412 hasRelatedWork W2030467719 @default.
- W4386085412 hasRelatedWork W2061886669 @default.
- W4386085412 hasRelatedWork W2072067752 @default.
- W4386085412 hasRelatedWork W2169705930 @default.
- W4386085412 hasRelatedWork W2355325334 @default.
- W4386085412 hasRelatedWork W2375466314 @default.
- W4386085412 hasRelatedWork W2385878716 @default.
- W4386085412 hasRelatedWork W2511256575 @default.
- W4386085412 hasRelatedWork W774990620 @default.
- W4386085412 hasVolume "2023" @default.
- W4386085412 isParatext "false" @default.
- W4386085412 isRetracted "false" @default.
- W4386085412 workType "article" @default.