Matches in SemOpenAlex for { <https://semopenalex.org/work/W4386091639> ?p ?o ?g. }
- W4386091639 endingPage "2135" @default.
- W4386091639 startingPage "2135" @default.
- W4386091639 abstract "Cell growth is inhibited by abiotic stresses during industrial processes, which is a limitation of microbial cell factories. Microbes with robust phenotypes are critical for its maximizing the yield of the target products in industrial biotechnology. Currently, there are several reports on the enhanced production of industrial metabolite through the introduction of Deinococcal genes into host cells, which confers cellular robustness. Deinococcus is known for its unique genetic function thriving in extreme environments such as radiation, UV, and oxidants. In this study, we established that Deinococcus proteolyticus showed greater resistance to oxidation and UV-C than commonly used D. radiodurans. By screening the genomic library of D. proteolyticus, we isolated a gene (deipr_0871) encoding a response regulator, which not only enhanced oxidative stress, but also promoted the growth of the recombinant E. coli strain. The transcription analysis indicated that the heterologous expression of deipr_0871 upregulated oxidative-stress-related genes such as ahpC and sodA, and acetyl-CoA-accumulation-associated genes via soxS regulon. Deipr_0871 was applied to improve the production of the valuable metabolite, poly-3-hydroxybutyrate (PHB), in the synthetic E. coli strain, which lead to the remarkably higher PHB than the control strain. Therefore, the stress tolerance gene from D. proteolyticus should be used in the modification of E. coli for the production of PHB and other biomaterials" @default.
- W4386091639 created "2023-08-24" @default.
- W4386091639 creator A5022894054 @default.
- W4386091639 creator A5025851175 @default.
- W4386091639 creator A5055194121 @default.
- W4386091639 creator A5074612002 @default.
- W4386091639 creator A5089709908 @default.
- W4386091639 creator A5091702326 @default.
- W4386091639 date "2023-08-23" @default.
- W4386091639 modified "2023-10-17" @default.
- W4386091639 title "Deionococcus proteotlycius Genomic Library Exploration Enhances Oxidative Stress Resistance and Poly-3-hydroxybutyrate Production in Recombinant Escherichia coli" @default.
- W4386091639 cites W1487254759 @default.
- W4386091639 cites W1740334656 @default.
- W4386091639 cites W1982719136 @default.
- W4386091639 cites W1985838015 @default.
- W4386091639 cites W1999312158 @default.
- W4386091639 cites W2006479463 @default.
- W4386091639 cites W2010983045 @default.
- W4386091639 cites W2013905005 @default.
- W4386091639 cites W2019303832 @default.
- W4386091639 cites W2024436299 @default.
- W4386091639 cites W2045204781 @default.
- W4386091639 cites W2060382254 @default.
- W4386091639 cites W2069480047 @default.
- W4386091639 cites W2077413381 @default.
- W4386091639 cites W2085796061 @default.
- W4386091639 cites W2086652584 @default.
- W4386091639 cites W2096093282 @default.
- W4386091639 cites W2112364185 @default.
- W4386091639 cites W2113204163 @default.
- W4386091639 cites W2115777587 @default.
- W4386091639 cites W2126294842 @default.
- W4386091639 cites W2129217817 @default.
- W4386091639 cites W2142758040 @default.
- W4386091639 cites W2144725934 @default.
- W4386091639 cites W2150095938 @default.
- W4386091639 cites W2157979510 @default.
- W4386091639 cites W2160437288 @default.
- W4386091639 cites W2161834936 @default.
- W4386091639 cites W2169528116 @default.
- W4386091639 cites W2170762473 @default.
- W4386091639 cites W2191582254 @default.
- W4386091639 cites W2311203695 @default.
- W4386091639 cites W2417499812 @default.
- W4386091639 cites W2621201488 @default.
- W4386091639 cites W2897540875 @default.
- W4386091639 cites W2921334440 @default.
- W4386091639 cites W2996941012 @default.
- W4386091639 cites W3011868083 @default.
- W4386091639 cites W3092999046 @default.
- W4386091639 cites W3100584158 @default.
- W4386091639 cites W3111995113 @default.
- W4386091639 cites W3197442830 @default.
- W4386091639 cites W4220817418 @default.
- W4386091639 cites W4284879330 @default.
- W4386091639 doi "https://doi.org/10.3390/microorganisms11092135" @default.
- W4386091639 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/37763980" @default.
- W4386091639 hasPublicationYear "2023" @default.
- W4386091639 type Work @default.
- W4386091639 citedByCount "0" @default.
- W4386091639 crossrefType "journal-article" @default.
- W4386091639 hasAuthorship W4386091639A5022894054 @default.
- W4386091639 hasAuthorship W4386091639A5025851175 @default.
- W4386091639 hasAuthorship W4386091639A5055194121 @default.
- W4386091639 hasAuthorship W4386091639A5074612002 @default.
- W4386091639 hasAuthorship W4386091639A5089709908 @default.
- W4386091639 hasAuthorship W4386091639A5091702326 @default.
- W4386091639 hasBestOaLocation W43860916391 @default.
- W4386091639 hasConcept C104317684 @default.
- W4386091639 hasConcept C2776519032 @default.
- W4386091639 hasConcept C54355233 @default.
- W4386091639 hasConcept C547475151 @default.
- W4386091639 hasConcept C62361671 @default.
- W4386091639 hasConcept C86803240 @default.
- W4386091639 hasConcept C89423630 @default.
- W4386091639 hasConceptScore W4386091639C104317684 @default.
- W4386091639 hasConceptScore W4386091639C2776519032 @default.
- W4386091639 hasConceptScore W4386091639C54355233 @default.
- W4386091639 hasConceptScore W4386091639C547475151 @default.
- W4386091639 hasConceptScore W4386091639C62361671 @default.
- W4386091639 hasConceptScore W4386091639C86803240 @default.
- W4386091639 hasConceptScore W4386091639C89423630 @default.
- W4386091639 hasFunder F4320322120 @default.
- W4386091639 hasIssue "9" @default.
- W4386091639 hasLocation W43860916391 @default.
- W4386091639 hasLocation W43860916392 @default.
- W4386091639 hasOpenAccess W4386091639 @default.
- W4386091639 hasPrimaryLocation W43860916391 @default.
- W4386091639 hasRelatedWork W1940806326 @default.
- W4386091639 hasRelatedWork W1969537517 @default.
- W4386091639 hasRelatedWork W2062480283 @default.
- W4386091639 hasRelatedWork W2148099665 @default.
- W4386091639 hasRelatedWork W2338688932 @default.
- W4386091639 hasRelatedWork W2361149789 @default.
- W4386091639 hasRelatedWork W2365391791 @default.
- W4386091639 hasRelatedWork W3135563671 @default.
- W4386091639 hasRelatedWork W4287541452 @default.
- W4386091639 hasRelatedWork W2107923379 @default.