FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4386102225> ?p ?o ?g. }

• W4386102225 endingPage "e376587c" @default.
• W4386102225 startingPage "e376587c" @default.
• W4386102225 abstract "Topic: 20. Lymphoma Biology & Translational Research Background: Introduction of microRNA profiling revealed a decrease in the level of p53-responsive oncosuppressive microRNAs miR-34a, miR-34b, miR-34c, miR-129 and miR-203 in lymphoma tumor tissue in comparison with normal lymphoid tissue. It is known that mutations in the DNA-binding domain of the TP53 gene responsible for the decreased expression of these molecules not only due to the absence of transcription-dependent activation, but also due to slowing down the processing of microRNAs in the nucleus. Most microRNA genes are located in CpG-rich regions, however, data on the frequency of methylation of MIR-34A, MIR-34B/C, MIR-129-2 and MIR-203 genes in lymphomas are very scarce. Aims: The aim of this study was to conduct a comprehensive analysis of the methylation of the p53-responsive microRNA genes MIR-34A, MIR-34B/C, MIR-203 and MIR-129-2, mutations in the TP53 gene DNA-binding domain and impairment of the TP53 polyadenylation signal in diffuse large B-cell lymphoma (DLBCL). Methods: 136 DNA samples isolated from tumor tissue of patients with DLBCL and 11 DNA samples obtained from lymph nodes with reactive B-cell follicular hyperplasia were analyzed. The methylation status of MIR-203 and MIR-129-2 genes was determined by the method of methyl-specific PCR, MIR-34A and MIR-34B/C genes by the method of methyl–sensitive analysis of high-resolution melting curves. In tumor samples, destruction of the polyadenylation signal was performed by rs78378222 genotyping by polymerase chain reaction with restriction fragment length polymorphism, and the nucleotide sequence of the TP53 DNA-binding domain was determined by capillary direct sequencing by Sanger. A notion of the combined detection of the studied genes methylation was obtained using the OncoPrinter, the calculation of Log2 Odds Ratio and one-sided Fisher’s exact test (p-value) adjusted for multiple comparisons using the Benjamini-Hochberg procedure (q-value) was carried out. Results: The frequency of methylation of MIR-34A, MIR-34B/C, MIR-203 and MIR-129-2 and TP53 gene aberrations (mutations and destruction of the polyadenylation signal) was 23%, 55%, 66%, 65% and 21%, respectively. The detected methylation was tumor-specific. The absence of methylation of at least one of the studied microRNA genes occurred only in 15/136 (11.0%) cases of lymphoma, while in the vast majority of samples, methylation was combined. Thus, in 15/136 (11.1%) cases methylation of all 4, and in 60/136 (44.1%) - 3, and in 33/136 (24.3%) - 2 from the analyzed genes took place. At the same time it was shown that methylation of the analyzed p53-responsive microRNA genes and aberrations in the TP53 gene in the DLBCL tumor tissue were independent events (table). The analysis of MIR-34A, MIR-34В/С, MIR-203 and MIR-129-2 genes methylation and TP53 gene aberrations by the OncoPrinter - Gene methylation Gene aberrations p-value q-value Tendency MIR-34A TP53 0,297 0,424 Mutual exclusivity MIR-129-2 TP53 0,412 0,458 Mutual exclusivity MIR-34B/C TP53 0,264 0,424 Mutual exclusivity MIR-203 TP53 0,225 0,424 Mutual exclusivity Summary/Conclusion: Along with the TP53 gene aberrations tumor specific methylation may cause a decrease in the expression of miR-34a, miR-34b, miR-34c, miR-129 and miR-203 in DLBCL. It can be assumed that the combined methylation of the MIR-203, MIR-129-2 and MIR-34B/C genes, as well as the MIR-34B/C and MIR-34A pairs, potentially has a more pronounced pro-tumor effect due to the presence of common targets in the microRNAs encoded by them. Keywords: Gene mutation, Epigenetic, Diffuse large B cell lymphoma, TP53" @default.
• W4386102225 created "2023-08-24" @default.
• W4386102225 creator A5003229183 @default.
• W4386102225 creator A5006788230 @default.
• W4386102225 creator A5033533443 @default.
• W4386102225 creator A5058135022 @default.
• W4386102225 creator A5061406585 @default.
• W4386102225 creator A5062117331 @default.
• W4386102225 date "2023-08-01" @default.
• W4386102225 modified "2023-10-01" @default.
• W4386102225 title "PB2378: COMPREHENSIVE ANALYSIS OF P53-RESPONSIVE MIRNA’S GENES METHYLATION, TP53 GENE MUTATIONS AND POLYADENYLATION SIGNAL DESTRUCTION IN DIFFUSE LARGE B-CELL LYMPHOMA TUMOR TISSUE." @default.
• W4386102225 doi "https://doi.org/10.1097/01.hs9.0000976228.37658.7c" @default.
• W4386102225 hasPublicationYear "2023" @default.
• W4386102225 type Work @default.
• W4386102225 citedByCount "0" @default.
• W4386102225 crossrefType "journal-article" @default.
• W4386102225 hasAuthorship W4386102225A5003229183 @default.
• W4386102225 hasAuthorship W4386102225A5006788230 @default.
• W4386102225 hasAuthorship W4386102225A5033533443 @default.
• W4386102225 hasAuthorship W4386102225A5058135022 @default.
• W4386102225 hasAuthorship W4386102225A5061406585 @default.
• W4386102225 hasAuthorship W4386102225A5062117331 @default.
• W4386102225 hasBestOaLocation W43861022251 @default.
• W4386102225 hasConcept C104317684 @default.
• W4386102225 hasConcept C142575336 @default.
• W4386102225 hasConcept C145059251 @default.
• W4386102225 hasConcept C150194340 @default.
• W4386102225 hasConcept C153911025 @default.
• W4386102225 hasConcept C190727270 @default.
• W4386102225 hasConcept C203014093 @default.
• W4386102225 hasConcept C2778559949 @default.
• W4386102225 hasConcept C2779338263 @default.
• W4386102225 hasConcept C33288867 @default.
• W4386102225 hasConcept C502942594 @default.
• W4386102225 hasConcept C54355233 @default.
• W4386102225 hasConcept C86803240 @default.
• W4386102225 hasConceptScore W4386102225C104317684 @default.
• W4386102225 hasConceptScore W4386102225C142575336 @default.
• W4386102225 hasConceptScore W4386102225C145059251 @default.
• W4386102225 hasConceptScore W4386102225C150194340 @default.
• W4386102225 hasConceptScore W4386102225C153911025 @default.
• W4386102225 hasConceptScore W4386102225C190727270 @default.
• W4386102225 hasConceptScore W4386102225C203014093 @default.
• W4386102225 hasConceptScore W4386102225C2778559949 @default.
• W4386102225 hasConceptScore W4386102225C2779338263 @default.
• W4386102225 hasConceptScore W4386102225C33288867 @default.
• W4386102225 hasConceptScore W4386102225C502942594 @default.
• W4386102225 hasConceptScore W4386102225C54355233 @default.
• W4386102225 hasConceptScore W4386102225C86803240 @default.
• W4386102225 hasIssue "S3" @default.
• W4386102225 hasLocation W43861022251 @default.
• W4386102225 hasOpenAccess W4386102225 @default.
• W4386102225 hasPrimaryLocation W43861022251 @default.
• W4386102225 hasRelatedWork W2009966535 @default.
• W4386102225 hasRelatedWork W2030030155 @default.
• W4386102225 hasRelatedWork W2319142430 @default.
• W4386102225 hasRelatedWork W2561201516 @default.
• W4386102225 hasRelatedWork W2922963799 @default.
• W4386102225 hasRelatedWork W2986632009 @default.
• W4386102225 hasRelatedWork W3030099995 @default.
• W4386102225 hasRelatedWork W4220750200 @default.
• W4386102225 hasRelatedWork W4283027398 @default.
• W4386102225 hasRelatedWork W4290804149 @default.
• W4386102225 hasVolume "7" @default.
• W4386102225 isParatext "false" @default.
• W4386102225 isRetracted "false" @default.
• W4386102225 workType "article" @default.