FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4386117002> ?p ?o ?g. }

• W4386117002 endingPage "12" @default.
• W4386117002 startingPage "1" @default.
• W4386117002 abstract "To characterize the in vivo effects of Dermatophagoides pteronyssinus 1 (DerP1) in mice and determine the underlying NLRP3 inflammasome-mediated pyroptosis signaling mechanisms in the human corneal epithelial cells (HCECs).DerP1 was used to induce allergic conjunctivitis in C57 mice. HCECs were sensitized with DerP1 in vitro to mimic their condition observed in allergic conjunctivitis in vivo. Transmission electron microscopy was used to evaluate pyroptosis in the HCECs, enzyme-linked immunosorbent assays to assess interleukin (IL)-33, IL-1β and IL-4 levels, flow cytometry to detect the proportion of Th2 cells, MTT assays to assess cell metabolic activity, immunofluorescence to evaluate the effects of DerP1 on functional HCEC phenotypes, and Western blot assays to detect the expression of NOD-like receptor family pyrin domain-containing 3 (NLRP3), gasdermin D (GSDMD), N-terminal fragment of GSDMD (GSDMD-N), pro-caspase-1, cleaved caspase-1, IL-1β, and IL-33. IL-33 expression in the HCECs was knocked down via lentivirus transfection.In vivo, DerP1 promotes pyroptosis, production of Th2 inflammatory cytokines and IL-33, and NLRP3 activation in mouse corneas. In vitro, pyroptotic bodies were found in the HCECs after sensitization with DerP1. Various concentrations of DerP1 increased the expression levels of NLRP3, GSDMD, GSDMD-N, pro-caspase-1, cleaved caspase-1, and IL-1β in the HCECs, with the largest increase observed after exposure to 20 µM DerP1. In vitro, recombinant human IL-33 mediated the expression of pyroptotic biomarkers in the HCECs, whereas IL-33 silencing diminished 20 µM DerP1-induced increase in their expression levels.DerP1 induces pyroptosis and allergic conjunctivitis, the expression of Th2 inflammatory cytokines, NLRP3 activation, and IL-33 in mouse corneas in our model. These effects would attribute to its activating NLRP3-GSDMD signaling pathway axis via enhancing IL-33 expression in HCECs." @default.
• W4386117002 created "2023-08-25" @default.
• W4386117002 creator A5069628890 @default.
• W4386117002 creator A5088870232 @default.
• W4386117002 creator A5090495247 @default.
• W4386117002 date "2023-08-25" @default.
• W4386117002 modified "2023-09-27" @default.
• W4386117002 title "<i>Dermatophagoides pteronyssinus 1</i> (DerP1) May Trigger NLRP3-Mediated Corneal Epithelial Cell Pyroptosis by Elevating Interleukin-33 Expression Levels" @default.
• W4386117002 cites W1516197123 @default.
• W4386117002 cites W1575966267 @default.
• W4386117002 cites W1864325817 @default.
• W4386117002 cites W1970107624 @default.
• W4386117002 cites W1973976588 @default.
• W4386117002 cites W1981873827 @default.
• W4386117002 cites W1988035869 @default.
• W4386117002 cites W2013907285 @default.
• W4386117002 cites W2044684461 @default.
• W4386117002 cites W2061946715 @default.
• W4386117002 cites W2063557080 @default.
• W4386117002 cites W2071517396 @default.
• W4386117002 cites W2111525045 @default.
• W4386117002 cites W2116135404 @default.
• W4386117002 cites W2128564051 @default.
• W4386117002 cites W2130509509 @default.
• W4386117002 cites W2165235005 @default.
• W4386117002 cites W2165774906 @default.
• W4386117002 cites W2167492366 @default.
• W4386117002 cites W2167734079 @default.
• W4386117002 cites W2175794645 @default.
• W4386117002 cites W2268195430 @default.
• W4386117002 cites W2346690549 @default.
• W4386117002 cites W2416989812 @default.
• W4386117002 cites W2771178586 @default.
• W4386117002 cites W2777420188 @default.
• W4386117002 cites W2779261498 @default.
• W4386117002 cites W2898545691 @default.
• W4386117002 cites W2907424910 @default.
• W4386117002 cites W2943293743 @default.
• W4386117002 cites W2970602413 @default.
• W4386117002 cites W2977578303 @default.
• W4386117002 cites W2982061867 @default.
• W4386117002 cites W2983652778 @default.
• W4386117002 cites W2985879784 @default.
• W4386117002 cites W3045978970 @default.
• W4386117002 cites W3087297820 @default.
• W4386117002 cites W3101453601 @default.
• W4386117002 cites W3134056000 @default.
• W4386117002 cites W3134632803 @default.
• W4386117002 cites W3166602798 @default.
• W4386117002 cites W3168160809 @default.
• W4386117002 cites W4313305824 @default.
• W4386117002 doi "https://doi.org/10.1080/02713683.2023.2250583" @default.
• W4386117002 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/37615401" @default.
• W4386117002 hasPublicationYear "2023" @default.
• W4386117002 type Work @default.
• W4386117002 citedByCount "0" @default.
• W4386117002 crossrefType "journal-article" @default.
• W4386117002 hasAuthorship W4386117002A5069628890 @default.
• W4386117002 hasAuthorship W4386117002A5088870232 @default.
• W4386117002 hasAuthorship W4386117002A5090495247 @default.
• W4386117002 hasConcept C104317684 @default.
• W4386117002 hasConcept C139964883 @default.
• W4386117002 hasConcept C150903083 @default.
• W4386117002 hasConcept C153911025 @default.
• W4386117002 hasConcept C17172800 @default.
• W4386117002 hasConcept C185592680 @default.
• W4386117002 hasConcept C203014093 @default.
• W4386117002 hasConcept C207001950 @default.
• W4386117002 hasConcept C26291073 @default.
• W4386117002 hasConcept C2776415932 @default.
• W4386117002 hasConcept C2776914184 @default.
• W4386117002 hasConcept C2777209026 @default.
• W4386117002 hasConcept C553184892 @default.
• W4386117002 hasConcept C55493867 @default.
• W4386117002 hasConcept C86803240 @default.
• W4386117002 hasConceptScore W4386117002C104317684 @default.
• W4386117002 hasConceptScore W4386117002C139964883 @default.
• W4386117002 hasConceptScore W4386117002C150903083 @default.
• W4386117002 hasConceptScore W4386117002C153911025 @default.
• W4386117002 hasConceptScore W4386117002C17172800 @default.
• W4386117002 hasConceptScore W4386117002C185592680 @default.
• W4386117002 hasConceptScore W4386117002C203014093 @default.
• W4386117002 hasConceptScore W4386117002C207001950 @default.
• W4386117002 hasConceptScore W4386117002C26291073 @default.
• W4386117002 hasConceptScore W4386117002C2776415932 @default.
• W4386117002 hasConceptScore W4386117002C2776914184 @default.
• W4386117002 hasConceptScore W4386117002C2777209026 @default.
• W4386117002 hasConceptScore W4386117002C553184892 @default.
• W4386117002 hasConceptScore W4386117002C55493867 @default.
• W4386117002 hasConceptScore W4386117002C86803240 @default.
• W4386117002 hasLocation W43861170021 @default.
• W4386117002 hasLocation W43861170022 @default.
• W4386117002 hasOpenAccess W4386117002 @default.
• W4386117002 hasPrimaryLocation W43861170021 @default.
• W4386117002 hasRelatedWork W2164406361 @default.
• W4386117002 hasRelatedWork W2756924764 @default.
• W4386117002 hasRelatedWork W2775971119 @default.
• W4386117002 hasRelatedWork W2944880367 @default.

• next