FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4386156629> ?p ?o ?g. }

• W4386156629 abstract "ABSTRACT Mechanosensitive (MS) channels act to protect the cytoplasmic membrane (CM) of living cells from environmental changes in osmolarity. In this report, we demonstrate the use of time-resolved second-harmonic light scattering (SHS) as a means of experimentally observing the relative state (open vs. closed) of MS channels in living bacteria suspended in different buffer solutions. Specifically, the state of the MS channels was selectively controlled by changing the composition of the suspension medium, inducing either a transient or persistent osmotic shock. SHS was then used to monitor transport of the SHG-active cation, malachite green (MG) across the bacterial CM. When MS channels were forced open, MG cations were able to cross the CM at a rate at least two orders of magnitude faster compared to when the MS channels were closed. These observations were corroborated using both numerical model simulations and complementary fluorescence experiments, in which the propensity for the CM impermeant cation, propidium to stain cells was shown to be contingent upon the relative state of the MS channels (i.e., cells with open MS channels fluoresced red, cells with closed MS channels did not). Application of time-resolved SHS to experimentally distinguish MS channels opened via osmotic shock vs. chemical activation, as well as a general comparison to the patch-clamp method is discussed." @default.
• W4386156629 created "2023-08-26" @default.
• W4386156629 creator A5009564631 @default.
• W4386156629 creator A5051230831 @default.
• W4386156629 creator A5089425570 @default.
• W4386156629 date "2023-08-25" @default.
• W4386156629 modified "2023-10-16" @default.
• W4386156629 title "Observing Mechanosensitive Channels in Action in Living Bacteria" @default.
• W4386156629 cites W1540556961 @default.
• W4386156629 cites W1622986665 @default.
• W4386156629 cites W1910799836 @default.
• W4386156629 cites W1966144406 @default.
• W4386156629 cites W1967597269 @default.
• W4386156629 cites W1969823298 @default.
• W4386156629 cites W1979856671 @default.
• W4386156629 cites W1981740875 @default.
• W4386156629 cites W1986514556 @default.
• W4386156629 cites W1992575467 @default.
• W4386156629 cites W2005294076 @default.
• W4386156629 cites W2008305695 @default.
• W4386156629 cites W2008541744 @default.
• W4386156629 cites W2008690725 @default.
• W4386156629 cites W2008897416 @default.
• W4386156629 cites W2009667219 @default.
• W4386156629 cites W2010843435 @default.
• W4386156629 cites W2012770784 @default.
• W4386156629 cites W2014573882 @default.
• W4386156629 cites W2014895510 @default.
• W4386156629 cites W2017832591 @default.
• W4386156629 cites W2019356890 @default.
• W4386156629 cites W2021237708 @default.
• W4386156629 cites W2025720625 @default.
• W4386156629 cites W2026236999 @default.
• W4386156629 cites W2033451549 @default.
• W4386156629 cites W2033824709 @default.
• W4386156629 cites W2033980199 @default.
• W4386156629 cites W2038254827 @default.
• W4386156629 cites W2038645762 @default.
• W4386156629 cites W2048009063 @default.
• W4386156629 cites W2049098323 @default.
• W4386156629 cites W2053357507 @default.
• W4386156629 cites W2055465627 @default.
• W4386156629 cites W2056248793 @default.
• W4386156629 cites W2056383020 @default.
• W4386156629 cites W2060263193 @default.
• W4386156629 cites W2061659241 @default.
• W4386156629 cites W2062316329 @default.
• W4386156629 cites W2064447912 @default.
• W4386156629 cites W2069567089 @default.
• W4386156629 cites W2071834094 @default.
• W4386156629 cites W2078446467 @default.
• W4386156629 cites W2080936114 @default.
• W4386156629 cites W2082894283 @default.
• W4386156629 cites W2086354121 @default.
• W4386156629 cites W2087389914 @default.
• W4386156629 cites W2089799023 @default.
• W4386156629 cites W2089894835 @default.
• W4386156629 cites W2092670028 @default.
• W4386156629 cites W2097296613 @default.
• W4386156629 cites W2112956889 @default.
• W4386156629 cites W2118116766 @default.
• W4386156629 cites W2118649820 @default.
• W4386156629 cites W2121209300 @default.
• W4386156629 cites W2125942201 @default.
• W4386156629 cites W2130833873 @default.
• W4386156629 cites W2134047940 @default.
• W4386156629 cites W2134451014 @default.
• W4386156629 cites W2141982593 @default.
• W4386156629 cites W2145986958 @default.
• W4386156629 cites W2147711662 @default.
• W4386156629 cites W2149229791 @default.
• W4386156629 cites W2149862252 @default.
• W4386156629 cites W2150405124 @default.
• W4386156629 cites W2159772052 @default.
• W4386156629 cites W2172246975 @default.
• W4386156629 cites W2320407793 @default.
• W4386156629 cites W2328111744 @default.
• W4386156629 cites W2418802224 @default.
• W4386156629 cites W2518472576 @default.
• W4386156629 cites W2522100620 @default.
• W4386156629 cites W2544894111 @default.
• W4386156629 cites W2793340903 @default.
• W4386156629 cites W2921134788 @default.
• W4386156629 cites W2923709388 @default.
• W4386156629 cites W2937644387 @default.
• W4386156629 cites W2973521418 @default.
• W4386156629 cites W3157435061 @default.
• W4386156629 cites W3204342372 @default.
• W4386156629 cites W4211198974 @default.
• W4386156629 cites W4211264697 @default.
• W4386156629 cites W4221100591 @default.
• W4386156629 cites W4239258735 @default.
• W4386156629 cites W4309666648 @default.
• W4386156629 cites W4362734749 @default.
• W4386156629 cites W794697133 @default.
• W4386156629 doi "https://doi.org/10.1101/2023.08.24.554739" @default.
• W4386156629 hasPublicationYear "2023" @default.
• W4386156629 type Work @default.
• W4386156629 citedByCount "0" @default.
• W4386156629 crossrefType "posted-content" @default.

• next