FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4386157187> ?p ?o ?g. }

• W4386157187 abstract "Background Bovine mastitis is a common disease of dairy cattle causing major economic losses due to reduced yield and poor quality of milk worldwide. The current investigation aimed to gain insight into the genetic diversity, antimicrobial resistance profiles and virulence associated factors of Staphylococcus ( S. ) aureus isolated from clinical bovine mastitis in dairy farms in Thuringia, Germany. Methods Forty Staphylococcus aureus isolates collected from clinical bovine mastitis cases from 17 Thuringian dairy farms were phenotyped and genetically characterized using whole genome sequencing. Results Out of 40 S. aureus , 30 (75%) were confirmed as methicillin resistant isolates. The isolates showed elevated antimicrobial resistance against penicillin, tetracycline and oxacillin, i.e., 77.5, 77.5, and 75%, respectively. Lower resistance rates were found against moxifloxacin, ciprofloxacin, gentamicin and trimethoprim/sulfamethoxazole, i.e., 35, 35, 30, and 22.5%, respectively. While resistance against clindamycin and erythromycin was rarely found (5 and 2.5%, respectively). All isolates were susceptible to linezolid, teicoplanin, vancomycin, tigecycline, fosfomycin, fusidic acid and rifampicin. These isolates were further allocated into five different sequence types: ST398 ( n = 31), ST1074 ( n = 4), ST504 ( n = 3), ST582 (CC15) ( n = 1) and ST479 ( n = 1). These isolates were also assigned to seven clusters with up to 100 SNP which has facilitated geographical mapping and epidemiological distribution in Thuringia. Strains belonging to ST398 were classified into clusters 1, 2, 3, 4 and 7. The isolates of ST504 were of cluster 5, those of ST1074 were belonging to cluster 6. Resistance genes bla Z, bla I and bla R associated with penicillin resistance were found in 32 (80%) strains, all except one were belonging to ST398. Methicillin resistance associated mec A was identified in 30 (96.8%) isolates of ST398. All tetracycline and erythromycin resistant isolates were of ST398, and all harbored both tet M and erm A. About 90.3% of tetracycline resistant isolates assigned to ST398 were also carrying tet K gene. The point mutations par C_S80F, gyr A_S84L and par C_S80Y in gyr A and par C associated with quinolone resistance were found in all phenotypically resistant isolates to ciprofloxacin and moxifloxacin ( n = 14). Sixty-eight virulence genes were identified among isolates. Both luk D/E and lukM /F-PV-P83 were identified in 22.5% of isolates, all were non-ST398. Conclusion In this study, ST398 had the highest potential to cause disease and had a massive prevalence in bovine mastitis cases. Five different sequence types and seven clusters were identified in the federal state of Thuringia. The circulation of some clusters in the same region over several years shows the persistence of cluster-associated infection despite the intensive veterinary care. On the other hand, some regions had different clusters at the same year or in different consecutive years. Different sequence types and associated different clusters of S. aureus were geographically widely distributed among dairy farms in Thuringia. The findings of this study show that various clusters have the potential to spread over a large geographical scale. The detection of LA-MRSA on dairy farms, which is known for cabapility to widely spread among different groups of animals, humans and their environment urges for the implementation of national wide strategic programs. The identification of CA-MRSA among the isolates such as ST398 poses a significant risk for the transmission of such strains between animals and humans on dairy farms." @default.
• W4386157187 created "2023-08-26" @default.
• W4386157187 creator A5011337436 @default.
• W4386157187 creator A5019017859 @default.
• W4386157187 creator A5031249859 @default.
• W4386157187 creator A5046980156 @default.
• W4386157187 creator A5053406943 @default.
• W4386157187 creator A5062987320 @default.
• W4386157187 creator A5090278888 @default.
• W4386157187 creator A5092693427 @default.
• W4386157187 creator A5092693428 @default.
• W4386157187 creator A5092693429 @default.
• W4386157187 date "2023-08-25" @default.
• W4386157187 modified "2023-10-14" @default.
• W4386157187 title "Whole genome sequence-based analysis of Staphylococcus aureus isolated from bovine mastitis in Thuringia, Germany" @default.
• W4386157187 cites W114800816 @default.
• W4386157187 cites W1606656500 @default.
• W4386157187 cites W1884317460 @default.
• W4386157187 cites W1989898630 @default.
• W4386157187 cites W1996351142 @default.
• W4386157187 cites W2014527228 @default.
• W4386157187 cites W2016371877 @default.
• W4386157187 cites W2035197926 @default.
• W4386157187 cites W2049581978 @default.
• W4386157187 cites W2052084459 @default.
• W4386157187 cites W2073367176 @default.
• W4386157187 cites W2080289164 @default.
• W4386157187 cites W2096525273 @default.
• W4386157187 cites W2096532217 @default.
• W4386157187 cites W2097618796 @default.
• W4386157187 cites W2119497560 @default.
• W4386157187 cites W2120432544 @default.
• W4386157187 cites W2120902911 @default.
• W4386157187 cites W2127399963 @default.
• W4386157187 cites W2128981183 @default.
• W4386157187 cites W2141052558 @default.
• W4386157187 cites W2142432877 @default.
• W4386157187 cites W2158121761 @default.
• W4386157187 cites W2162549023 @default.
• W4386157187 cites W2316777574 @default.
• W4386157187 cites W2331362979 @default.
• W4386157187 cites W2529306918 @default.
• W4386157187 cites W2565829295 @default.
• W4386157187 cites W2597896394 @default.
• W4386157187 cites W2605135797 @default.
• W4386157187 cites W2607960658 @default.
• W4386157187 cites W2612402838 @default.
• W4386157187 cites W2617797655 @default.
• W4386157187 cites W2745444965 @default.
• W4386157187 cites W2751176812 @default.
• W4386157187 cites W2785724127 @default.
• W4386157187 cites W2789420429 @default.
• W4386157187 cites W2804953782 @default.
• W4386157187 cites W2947765638 @default.
• W4386157187 cites W2952079126 @default.
• W4386157187 cites W2967125766 @default.
• W4386157187 cites W2969549387 @default.
• W4386157187 cites W2976488130 @default.
• W4386157187 cites W2989943892 @default.
• W4386157187 cites W2990618091 @default.
• W4386157187 cites W3008666404 @default.
• W4386157187 cites W3037415594 @default.
• W4386157187 cites W3041160941 @default.
• W4386157187 cites W3091907857 @default.
• W4386157187 cites W3093984014 @default.
• W4386157187 cites W3119506673 @default.
• W4386157187 cites W3173693794 @default.
• W4386157187 cites W3185282221 @default.
• W4386157187 cites W4207067711 @default.
• W4386157187 cites W4220702126 @default.
• W4386157187 cites W4221051193 @default.
• W4386157187 cites W4248735723 @default.
• W4386157187 cites W4292171809 @default.
• W4386157187 doi "https://doi.org/10.3389/fmicb.2023.1216850" @default.
• W4386157187 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/37692386" @default.
• W4386157187 hasPublicationYear "2023" @default.
• W4386157187 type Work @default.
• W4386157187 citedByCount "0" @default.
• W4386157187 crossrefType "journal-article" @default.
• W4386157187 hasAuthorship W4386157187A5011337436 @default.
• W4386157187 hasAuthorship W4386157187A5019017859 @default.
• W4386157187 hasAuthorship W4386157187A5031249859 @default.
• W4386157187 hasAuthorship W4386157187A5046980156 @default.
• W4386157187 hasAuthorship W4386157187A5053406943 @default.
• W4386157187 hasAuthorship W4386157187A5062987320 @default.
• W4386157187 hasAuthorship W4386157187A5090278888 @default.
• W4386157187 hasAuthorship W4386157187A5092693427 @default.
• W4386157187 hasAuthorship W4386157187A5092693428 @default.
• W4386157187 hasAuthorship W4386157187A5092693429 @default.
• W4386157187 hasBestOaLocation W43861571871 @default.
• W4386157187 hasConcept C11701158 @default.
• W4386157187 hasConcept C159047783 @default.
• W4386157187 hasConcept C174802600 @default.
• W4386157187 hasConcept C2776344049 @default.
• W4386157187 hasConcept C2776703206 @default.
• W4386157187 hasConcept C2777027569 @default.
• W4386157187 hasConcept C2777052132 @default.
• W4386157187 hasConcept C2777458120 @default.
• W4386157187 hasConcept C2778980435 @default.
• W4386157187 hasConcept C2779489039 @default.

• next