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- W4386160408 abstract "Here, we present a detailed protocol to study the role of a human nuclear m6A RNA reader, YTHDC1, on chromatin-associated RNA targets. We describe steps for RNA extraction coupled to subnuclear fractionation to identify and study RNA-based regulations that take place in the chromatin-associated fraction. We then detail an RNA immunoprecipitation procedure adapted to identify chromatin-associated RNA targets. This protocol can be adapted to other human or mammalian chromatin-associated RNA binding proteins. For complete details on the use and execution of this protocol, please refer to Timcheva et al.1" @default.
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- W4386160408 date "2023-09-01" @default.
- W4386160408 modified "2023-10-04" @default.
- W4386160408 title "Protocol to study the role of a human nuclear m6A RNA reader on chromatin-associated RNA targets" @default.
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- W4386160408 doi "https://doi.org/10.1016/j.xpro.2023.102528" @default.
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