Matches in SemOpenAlex for { <https://semopenalex.org/work/W4386161722> ?p ?o ?g. }
- W4386161722 abstract "This study aims to demonstrate the cellular composition and underlying mechanisms in subchondral bone marrow lesions (BMLs) of knee osteoarthritis (OA).BMLs were assessed by MRI Osteoarthritis Knee Score (MOAKS)≥2. Bulk RNA-sequencing (bulk-seq) and BML-specific differentially expressed genes (DEGs) analysis were performed among subchondral bone samples (including OA-BML=3, paired OA-NBML=3; non-OA=3). The hub genes of BMLs were identified by verifying in independent datasets and multiple bioinformatic analyses. To further estimate cell-type composition of subchondral bone, we utilized two newly developed deconvolution algorithms (MuSiC, MCP-counter) in transcriptomic datasets, based on signatures from open-accessed single-cell RNA sequencing (scRNA-seq). Finally, competing endogenous RNA (ceRNA) and transcription factor (TF) networks were constructed through multiple predictive databases, and validated by public non-coding RNA profiles.A total of 86 BML-specific DEGs (up 79, down 7) were identified. IL11 and VCAN were identified as core hub genes. The has-miR-424-5p/lncRNA PVT1 was determined as crucial network, targeting IL11 and VCAN, respectively. More importantly, two deconvolution algorithms produced approximate estimations of cell-type composition, and the cluster of heterotopic-chondrocyte was discovered abundant in BMLs, and positively correlated with the expression of hub genes.IL11 and VCAN were identified as the core hub genes of BMLs, and their molecular networks were determined as well. We profiled the characteristics of subchondral bone at single-cell level and determined that the heterotopic-chondrocyte was abundant in BMLs and was closely linked to IL11 and VCAN. Our study may provide new insights into the microenvironment and pathological molecular mechanism of BMLs, and could lead to novel therapeutic strategies." @default.
- W4386161722 created "2023-08-26" @default.
- W4386161722 creator A5025754112 @default.
- W4386161722 creator A5029463866 @default.
- W4386161722 creator A5029946717 @default.
- W4386161722 creator A5030021804 @default.
- W4386161722 creator A5030967569 @default.
- W4386161722 creator A5033365996 @default.
- W4386161722 creator A5039347763 @default.
- W4386161722 creator A5041806098 @default.
- W4386161722 creator A5045439323 @default.
- W4386161722 creator A5049588864 @default.
- W4386161722 creator A5055115466 @default.
- W4386161722 creator A5056184564 @default.
- W4386161722 creator A5064842058 @default.
- W4386161722 creator A5074247805 @default.
- W4386161722 creator A5077721639 @default.
- W4386161722 creator A5079804152 @default.
- W4386161722 creator A5082262278 @default.
- W4386161722 creator A5084907853 @default.
- W4386161722 date "2023-08-25" @default.
- W4386161722 modified "2023-10-10" @default.
- W4386161722 title "Comprehensive analysis on subchondral bone marrow lesions of human osteoarthritis by integrating bulk and single-cell transcriptomes" @default.
- W4386161722 cites W1569946922 @default.
- W4386161722 cites W1836924425 @default.
- W4386161722 cites W1971493318 @default.
- W4386161722 cites W1997140718 @default.
- W4386161722 cites W1997637714 @default.
- W4386161722 cites W2021821328 @default.
- W4386161722 cites W2034251536 @default.
- W4386161722 cites W2061261019 @default.
- W4386161722 cites W2088054678 @default.
- W4386161722 cites W2109608328 @default.
- W4386161722 cites W2118903184 @default.
- W4386161722 cites W2132853845 @default.
- W4386161722 cites W2149110997 @default.
- W4386161722 cites W2156151010 @default.
- W4386161722 cites W2156763800 @default.
- W4386161722 cites W2176183018 @default.
- W4386161722 cites W2239019329 @default.
- W4386161722 cites W2328176404 @default.
- W4386161722 cites W2397360665 @default.
- W4386161722 cites W2533508881 @default.
- W4386161722 cites W2625972055 @default.
- W4386161722 cites W2770331207 @default.
- W4386161722 cites W2783490457 @default.
- W4386161722 cites W2888255347 @default.
- W4386161722 cites W2896157452 @default.
- W4386161722 cites W2907514116 @default.
- W4386161722 cites W2915567854 @default.
- W4386161722 cites W2935855188 @default.
- W4386161722 cites W2943182598 @default.
- W4386161722 cites W2950103694 @default.
- W4386161722 cites W2952240309 @default.
- W4386161722 cites W2991146640 @default.
- W4386161722 cites W3017561167 @default.
- W4386161722 cites W3022691252 @default.
- W4386161722 cites W3037315173 @default.
- W4386161722 cites W3043429347 @default.
- W4386161722 cites W3048879975 @default.
- W4386161722 cites W3092209489 @default.
- W4386161722 cites W3096429227 @default.
- W4386161722 cites W3108001297 @default.
- W4386161722 cites W3119762460 @default.
- W4386161722 cites W3126989368 @default.
- W4386161722 cites W3158185459 @default.
- W4386161722 cites W3158637956 @default.
- W4386161722 cites W3193910581 @default.
- W4386161722 cites W3209134411 @default.
- W4386161722 cites W3210206291 @default.
- W4386161722 cites W4200380121 @default.
- W4386161722 cites W4200606979 @default.
- W4386161722 cites W4210595879 @default.
- W4386161722 cites W4211067786 @default.
- W4386161722 cites W4220729278 @default.
- W4386161722 cites W4225568612 @default.
- W4386161722 cites W4252097774 @default.
- W4386161722 cites W4281742670 @default.
- W4386161722 cites W4307816170 @default.
- W4386161722 doi "https://doi.org/10.1186/s12891-023-06676-4" @default.
- W4386161722 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/37626330" @default.
- W4386161722 hasPublicationYear "2023" @default.
- W4386161722 type Work @default.
- W4386161722 citedByCount "0" @default.
- W4386161722 crossrefType "journal-article" @default.
- W4386161722 hasAuthorship W4386161722A5025754112 @default.
- W4386161722 hasAuthorship W4386161722A5029463866 @default.
- W4386161722 hasAuthorship W4386161722A5029946717 @default.
- W4386161722 hasAuthorship W4386161722A5030021804 @default.
- W4386161722 hasAuthorship W4386161722A5030967569 @default.
- W4386161722 hasAuthorship W4386161722A5033365996 @default.
- W4386161722 hasAuthorship W4386161722A5039347763 @default.
- W4386161722 hasAuthorship W4386161722A5041806098 @default.
- W4386161722 hasAuthorship W4386161722A5045439323 @default.
- W4386161722 hasAuthorship W4386161722A5049588864 @default.
- W4386161722 hasAuthorship W4386161722A5055115466 @default.
- W4386161722 hasAuthorship W4386161722A5056184564 @default.
- W4386161722 hasAuthorship W4386161722A5064842058 @default.
- W4386161722 hasAuthorship W4386161722A5074247805 @default.
- W4386161722 hasAuthorship W4386161722A5077721639 @default.