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- W4386202630 abstract "In the former report, the casein peptide TKLTEEEKNR (PfCN) exhibits strong thrombin inhibitory activity in vitro. Its absorption capabilities, however, are unclear. Therefore, we studied its absorption characteristics both in vivo and in vitro. PfCN was carried by cells from the apical chamber to the basolateral chamber via active translocation in Caco-2 cells. Meanwhile, it can also be transported by HUVECs. We found that PfCN can be taken up by HUVECs using confocal laser imaging. PfCN has been proven to have good absorption properties in in vivo experiments. After five minutes of oral treatment, PfCN was identified in the blood, peaking at 82.75 ± 36.52 ng/mL in 30 min. And PfCN vanished from the blood circulation after 120 min. According to in vivo experiments, excessive concentrations of PfCN will alter the permeability of HUVECs. As a result, there is a foundation for PfCN application in the food sector. Meanwhile, we also hope this article can give an idea to the researchers who studying the absorption of functional peptides." @default.
- W4386202630 created "2023-08-28" @default.
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- W4386202630 date "2023-11-01" @default.
- W4386202630 modified "2023-10-10" @default.
- W4386202630 title "Absorption characteristics and the effect on vascular endothelial cell permeability of an anticoagulant peptide" @default.
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- W4386202630 doi "https://doi.org/10.1016/j.foodres.2023.113405" @default.
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