FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4386203439> ?p ?o ?g. }

• W4386203439 abstract "Abstract Five enzymes of the archaeal hyperthermophilic family of disproportionating GH57 4-α-glucanotransferases have been studied till date. Our focus here lies upon three homologous members of this family: (i) PfuAmyGT from Pyrococcus furiosus (PF0272), (ii) TonAmyGT from Thermococcus onnurenius (B6YUX8), and (iii) TliAmyGT (TLGT) from Thermococcus litoralis (O32462). The polypeptide chain of each of these enzymes is approximately 655 residues long, folded into three distinct domains (1, 2 and 3), and assembled into a homodimer. Domain 1 is a beta/alpha barrel containing an aspartate known to function as a catalytic nucleophile in TLGT. Domain 2 (which is helical) and domain 3 (made up of beta sheets) are thought to be domains of unknown function (or DUFs). In PfuAmyGT and TonAmyGT, we have recently identified a catalytically-important aspartate upon a loop in domain 2. In PfuAmyGT, we demonstrate the presence of two additional catalytically-important (glutamate) residues in domain 1, in a companion paper. In this paper, our focus lies upon domain 3 which hosts a second binding site (SBS) for a glucan, at its domain-domain interface with domain 2. Using strategies involving studies of both (a) domains (or pairs of contiguous domains) extracted from PfuAmyGT, and (b) chimeric three-domain enzymes recombining analogous domains between PfuAmyGT and TonAmyGT, we demonstrate that domain 3 determines the choice of the preferred glucan that acts as a donor in the glucan transfer reaction." @default.
• W4386203439 created "2023-08-28" @default.
• W4386203439 creator A5006213497 @default.
• W4386203439 creator A5062194029 @default.
• W4386203439 creator A5073903148 @default.
• W4386203439 date "2023-08-26" @default.
• W4386203439 modified "2023-09-24" @default.
• W4386203439 title "Differences in the activities of domain-swapped chimeras of two homologous GH57 glucanotransferases suggest that a glucan-binding DUF could influence donor substrate specificity" @default.
• W4386203439 cites W1590913544 @default.
• W4386203439 cites W1803804183 @default.
• W4386203439 cites W1985794598 @default.
• W4386203439 cites W1988838703 @default.
• W4386203439 cites W1992596883 @default.
• W4386203439 cites W1999259201 @default.
• W4386203439 cites W2003048469 @default.
• W4386203439 cites W2022638594 @default.
• W4386203439 cites W2034767282 @default.
• W4386203439 cites W2038669138 @default.
• W4386203439 cites W2068302675 @default.
• W4386203439 cites W2080528210 @default.
• W4386203439 cites W2090028899 @default.
• W4386203439 cites W2105734162 @default.
• W4386203439 cites W2115974407 @default.
• W4386203439 cites W2123887225 @default.
• W4386203439 cites W2137450588 @default.
• W4386203439 cites W2155201844 @default.
• W4386203439 cites W2155267072 @default.
• W4386203439 cites W2156657683 @default.
• W4386203439 cites W2159341724 @default.
• W4386203439 cites W2167879487 @default.
• W4386203439 cites W2329337071 @default.
• W4386203439 cites W2585841483 @default.
• W4386203439 cites W2605716995 @default.
• W4386203439 cites W2804822363 @default.
• W4386203439 cites W2910217998 @default.
• W4386203439 cites W2920089551 @default.
• W4386203439 cites W2936418328 @default.
• W4386203439 doi "https://doi.org/10.1101/2023.08.25.554918" @default.
• W4386203439 hasPublicationYear "2023" @default.
• W4386203439 type Work @default.
• W4386203439 citedByCount "0" @default.
• W4386203439 crossrefType "posted-content" @default.
• W4386203439 hasAuthorship W4386203439A5006213497 @default.
• W4386203439 hasAuthorship W4386203439A5062194029 @default.
• W4386203439 hasAuthorship W4386203439A5073903148 @default.
• W4386203439 hasBestOaLocation W43862034391 @default.
• W4386203439 hasConcept C104317684 @default.
• W4386203439 hasConcept C10522644 @default.
• W4386203439 hasConcept C107824862 @default.
• W4386203439 hasConcept C111919701 @default.
• W4386203439 hasConcept C113027372 @default.
• W4386203439 hasConcept C134306372 @default.
• W4386203439 hasConcept C14036430 @default.
• W4386203439 hasConcept C153660203 @default.
• W4386203439 hasConcept C159547669 @default.
• W4386203439 hasConcept C167625842 @default.
• W4386203439 hasConcept C185592680 @default.
• W4386203439 hasConcept C25166345 @default.
• W4386203439 hasConcept C2776943354 @default.
• W4386203439 hasConcept C2780557392 @default.
• W4386203439 hasConcept C33923547 @default.
• W4386203439 hasConcept C36503486 @default.
• W4386203439 hasConcept C41008148 @default.
• W4386203439 hasConcept C47701112 @default.
• W4386203439 hasConcept C54355233 @default.
• W4386203439 hasConcept C550995028 @default.
• W4386203439 hasConcept C55493867 @default.
• W4386203439 hasConcept C71240020 @default.
• W4386203439 hasConcept C86803240 @default.
• W4386203439 hasConceptScore W4386203439C104317684 @default.
• W4386203439 hasConceptScore W4386203439C10522644 @default.
• W4386203439 hasConceptScore W4386203439C107824862 @default.
• W4386203439 hasConceptScore W4386203439C111919701 @default.
• W4386203439 hasConceptScore W4386203439C113027372 @default.
• W4386203439 hasConceptScore W4386203439C134306372 @default.
• W4386203439 hasConceptScore W4386203439C14036430 @default.
• W4386203439 hasConceptScore W4386203439C153660203 @default.
• W4386203439 hasConceptScore W4386203439C159547669 @default.
• W4386203439 hasConceptScore W4386203439C167625842 @default.
• W4386203439 hasConceptScore W4386203439C185592680 @default.
• W4386203439 hasConceptScore W4386203439C25166345 @default.
• W4386203439 hasConceptScore W4386203439C2776943354 @default.
• W4386203439 hasConceptScore W4386203439C2780557392 @default.
• W4386203439 hasConceptScore W4386203439C33923547 @default.
• W4386203439 hasConceptScore W4386203439C36503486 @default.
• W4386203439 hasConceptScore W4386203439C41008148 @default.
• W4386203439 hasConceptScore W4386203439C47701112 @default.
• W4386203439 hasConceptScore W4386203439C54355233 @default.
• W4386203439 hasConceptScore W4386203439C550995028 @default.
• W4386203439 hasConceptScore W4386203439C55493867 @default.
• W4386203439 hasConceptScore W4386203439C71240020 @default.
• W4386203439 hasConceptScore W4386203439C86803240 @default.
• W4386203439 hasLocation W43862034391 @default.
• W4386203439 hasOpenAccess W4386203439 @default.
• W4386203439 hasPrimaryLocation W43862034391 @default.
• W4386203439 hasRelatedWork W1974286739 @default.
• W4386203439 hasRelatedWork W1978012062 @default.
• W4386203439 hasRelatedWork W1995775434 @default.
• W4386203439 hasRelatedWork W2030786927 @default.
• W4386203439 hasRelatedWork W2073347761 @default.

• next