FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4386246439> ?p ?o ?g. }

• W4386246439 abstract "Human omentum-derived mesenchymal stem cells (hO-MSCs) possess great potential to differentiate into multiple lineages and have self-renewal capacity, allowing them to be utilized as patient-specific cell-based therapeutics. Although the use of various stem cell-derived β-cells has been proposed as a novel approach for treating diabetes mellitus, developing an efficient method to establish highly functional β-cells remains challenging.We aimed to develop a novel cell culture platform that utilizes a fibroblast growth factor 2 (FGF2)-immobilized matrix to regulate the adhesion and differentiation of hO-MSCs into insulin-producing β-cells via cell-matrix/cell-cell interactions. In our study, we evaluated the in vitro differentiation potential of hO-MSCs cultured on an FGF2-immobilized matrix and a round-bottom plate (RBP). Further, the in vivo therapeutic efficacy of the β-cells transplanted into kidney capsules was evaluated using animal models with streptozotocin (STZ)-induced diabetes.Our findings demonstrated that cells cultured on an FGF2-immobilized matrix could self-organize into insulin-producing β-cell progenitors, as evident from the upregulation of pancreatic β-cell-specific markers (PDX-1, Insulin, and Glut-2). Moreover, we observed significant upregulation of heparan sulfate proteoglycan, gap junction proteins (Cx36 and Cx43), and cell adhesion molecules (E-cadherin and Ncam1) in cells cultured on the FGF2-immobilized matrix. In addition, in vivo transplantation of differentiated β-cells into animal models of STZ-induced diabetes revealed their survival and engraftment as well as glucose-sensitive production of insulin within the host microenvironment, at over 4 weeks after transplantation.Our findings suggest that the FGF2-immobilized matrix can support initial cell adhesion, maturation, and glucose-stimulated insulin secretion within the host microenvironment. Such a cell culture platform can offer novel strategies to obtain functional pancreatic β-cells from patient-specific cell sources, ultimately enabling better treatment for diabetes mellitus." @default.
• W4386246439 created "2023-08-30" @default.
• W4386246439 creator A5001809317 @default.
• W4386246439 creator A5011446557 @default.
• W4386246439 creator A5013232529 @default.
• W4386246439 creator A5029128399 @default.
• W4386246439 creator A5040142782 @default.
• W4386246439 creator A5050277676 @default.
• W4386246439 creator A5054786425 @default.
• W4386246439 creator A5054835054 @default.
• W4386246439 creator A5054994372 @default.
• W4386246439 creator A5065006809 @default.
• W4386246439 creator A5069722067 @default.
• W4386246439 creator A5077868485 @default.
• W4386246439 creator A5087263866 @default.
• W4386246439 creator A5087588859 @default.
• W4386246439 date "2023-08-29" @default.
• W4386246439 modified "2023-10-06" @default.
• W4386246439 title "Self-organized insulin-producing β-cells differentiated from human omentum-derived stem cells and their in vivo therapeutic potential" @default.
• W4386246439 cites W1497803689 @default.
• W4386246439 cites W1580073633 @default.
• W4386246439 cites W1761645898 @default.
• W4386246439 cites W1827691446 @default.
• W4386246439 cites W1965045547 @default.
• W4386246439 cites W1965953554 @default.
• W4386246439 cites W1967202103 @default.
• W4386246439 cites W1967618429 @default.
• W4386246439 cites W1971756446 @default.
• W4386246439 cites W1981466203 @default.
• W4386246439 cites W1982510502 @default.
• W4386246439 cites W1985112613 @default.
• W4386246439 cites W1990033131 @default.
• W4386246439 cites W1993534485 @default.
• W4386246439 cites W2007541978 @default.
• W4386246439 cites W2008933976 @default.
• W4386246439 cites W2014473896 @default.
• W4386246439 cites W2025371271 @default.
• W4386246439 cites W2028641524 @default.
• W4386246439 cites W2035582064 @default.
• W4386246439 cites W2045559539 @default.
• W4386246439 cites W2056983875 @default.
• W4386246439 cites W2062367032 @default.
• W4386246439 cites W2062783006 @default.
• W4386246439 cites W2066885602 @default.
• W4386246439 cites W2093381564 @default.
• W4386246439 cites W2096556444 @default.
• W4386246439 cites W2107277218 @default.
• W4386246439 cites W2109538121 @default.
• W4386246439 cites W2111452665 @default.
• W4386246439 cites W2115389033 @default.
• W4386246439 cites W2117391590 @default.
• W4386246439 cites W2119304571 @default.
• W4386246439 cites W2119452287 @default.
• W4386246439 cites W2147186566 @default.
• W4386246439 cites W2148890861 @default.
• W4386246439 cites W2159999899 @default.
• W4386246439 cites W2168351266 @default.
• W4386246439 cites W2192080449 @default.
• W4386246439 cites W2228603639 @default.
• W4386246439 cites W2335692117 @default.
• W4386246439 cites W2342895842 @default.
• W4386246439 cites W2437812675 @default.
• W4386246439 cites W2545599580 @default.
• W4386246439 cites W2596680732 @default.
• W4386246439 cites W2622289277 @default.
• W4386246439 cites W2781883044 @default.
• W4386246439 cites W2783316531 @default.
• W4386246439 cites W2793816713 @default.
• W4386246439 cites W2913947571 @default.
• W4386246439 cites W2917580929 @default.
• W4386246439 cites W2922456138 @default.
• W4386246439 cites W2963480388 @default.
• W4386246439 cites W2978572002 @default.
• W4386246439 cites W3005090647 @default.
• W4386246439 cites W3006284965 @default.
• W4386246439 cites W3007716699 @default.
• W4386246439 cites W3037796306 @default.
• W4386246439 cites W3042191807 @default.
• W4386246439 cites W3103618045 @default.
• W4386246439 cites W3144181733 @default.
• W4386246439 cites W3193360748 @default.
• W4386246439 cites W3197355082 @default.
• W4386246439 cites W4200198734 @default.
• W4386246439 cites W4214638790 @default.
• W4386246439 cites W4220916556 @default.
• W4386246439 cites W4224737774 @default.
• W4386246439 cites W4226332522 @default.
• W4386246439 cites W4304588476 @default.
• W4386246439 cites W4313423874 @default.
• W4386246439 cites W4375866869 @default.
• W4386246439 doi "https://doi.org/10.1186/s40824-023-00419-1" @default.
• W4386246439 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/37644502" @default.
• W4386246439 hasPublicationYear "2023" @default.
• W4386246439 type Work @default.
• W4386246439 citedByCount "0" @default.
• W4386246439 crossrefType "journal-article" @default.
• W4386246439 hasAuthorship W4386246439A5001809317 @default.
• W4386246439 hasAuthorship W4386246439A5011446557 @default.
• W4386246439 hasAuthorship W4386246439A5013232529 @default.
• W4386246439 hasAuthorship W4386246439A5029128399 @default.

• next