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• W4386319802 endingPage "57" @default.
• W4386319802 startingPage "50" @default.
• W4386319802 abstract "Endothelin (ET)-1 is an endothelial-derived peptide that exerts biphasic effects on nitric oxide (NO) levels in endothelial cells such that acute exposure stimulates-while sustained exposure attenuates-NO production. Although the mechanism involved in the decrease in NO generation has been identified but the signaling involved in the acute increase in NO is still unresolved. This was the focus of this study. Our data indicate that exposing pulmonary arterial endothelial cells (PAEC) to ET-1 led to an increase in NO for up to 30min after which levels declined. These effects were attenuated by ET receptor antagonists. The increase in NO correlated with significant increases in pp60Src activity and increases in eNOS phosphorylation at Tyr83 and Ser1177. The ET-1 mediated increase in phosphorylation and NO generation were attenuated by the over-expression of a pp60Src dominant negative mutant. The increase in pp60Src activity correlated with a reduction in the interaction of Caveolin-1 with pp60Src and the calcineurin-mediated dephosphorylation of caveolin-1 at three previously unidentified sites: Thr91, Thr93, and Thr95. The calcineurin inhibitor, Tacrolimus, attenuated the acute increase in pp60Src activity induced by ET-1 and a calcineurin siRNA attenuated the ET-1 mediated increase in eNOS phosphorylation at Tyr83 and Ser1177 as well as the increase in NO. By using a Caveolin-1 celluSpot peptide array, we identified a peptide targeting a sequence located between aa 41–56 as the pp60Src binding region. This peptide fused to the TAT sequence was found to decrease caveolin-pp60Src interaction, increased pp60Src activity, increased eNOS pSer1177 and NO levels in PAEC and induce vasodilation in isolated aortic rings in wildtype but not eNOS knockout mice. Together, our data identify a novel mechanism by which ET-1 acutely increases NO via a calcineurin-mediated dephosphorylation of caveolin-1 and the subsequent stimulation of pp60Src activity, leading to increases in phosphorylation of eNOS at Tyr83 and Ser1177." @default.
• W4386319802 created "2023-09-01" @default.
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• W4386319802 date "2023-11-01" @default.
• W4386319802 modified "2023-09-27" @default.
• W4386319802 title "Endothelin-1 acutely increases nitric oxide production via the calcineurin mediated dephosphorylation of Caveolin-1" @default.
• W4386319802 cites W145898388 @default.
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• W4386319802 cites W1530167239 @default.
• W4386319802 cites W1557888154 @default.
• W4386319802 cites W1595102998 @default.
• W4386319802 cites W1600285621 @default.
• W4386319802 cites W1678800844 @default.
• W4386319802 cites W1967024806 @default.
• W4386319802 cites W1968959115 @default.
• W4386319802 cites W1971860517 @default.
• W4386319802 cites W1975532945 @default.
• W4386319802 cites W1976758368 @default.
• W4386319802 cites W1979780855 @default.
• W4386319802 cites W1979986181 @default.
• W4386319802 cites W1992705676 @default.
• W4386319802 cites W1993612535 @default.
• W4386319802 cites W2006920712 @default.
• W4386319802 cites W2016392169 @default.
• W4386319802 cites W2018752752 @default.
• W4386319802 cites W2019615010 @default.
• W4386319802 cites W2021295284 @default.
• W4386319802 cites W2022568707 @default.
• W4386319802 cites W2024078613 @default.
• W4386319802 cites W2024286560 @default.
• W4386319802 cites W2036886710 @default.
• W4386319802 cites W2037632643 @default.
• W4386319802 cites W2042158231 @default.
• W4386319802 cites W2043636613 @default.
• W4386319802 cites W2047166224 @default.
• W4386319802 cites W2048635427 @default.
• W4386319802 cites W2050949616 @default.
• W4386319802 cites W2057257230 @default.
• W4386319802 cites W2059369537 @default.
• W4386319802 cites W2063110082 @default.
• W4386319802 cites W2065399599 @default.
• W4386319802 cites W2065440690 @default.
• W4386319802 cites W2066290626 @default.
• W4386319802 cites W2068820862 @default.
• W4386319802 cites W2070002365 @default.
• W4386319802 cites W2073598808 @default.
• W4386319802 cites W2074176407 @default.
• W4386319802 cites W2074858413 @default.
• W4386319802 cites W2086227484 @default.
• W4386319802 cites W2088605918 @default.
• W4386319802 cites W2092437783 @default.
• W4386319802 cites W2095480781 @default.
• W4386319802 cites W2095495393 @default.
• W4386319802 cites W2096401506 @default.
• W4386319802 cites W2097835607 @default.
• W4386319802 cites W2103790130 @default.
• W4386319802 cites W2103815234 @default.
• W4386319802 cites W2106181213 @default.
• W4386319802 cites W2106337149 @default.
• W4386319802 cites W2114750148 @default.
• W4386319802 cites W2116709740 @default.
• W4386319802 cites W2118780544 @default.
• W4386319802 cites W2121858640 @default.
• W4386319802 cites W2123830444 @default.
• W4386319802 cites W2124024199 @default.
• W4386319802 cites W2133349052 @default.
• W4386319802 cites W2135297555 @default.
• W4386319802 cites W2135339304 @default.
• W4386319802 cites W2140245242 @default.
• W4386319802 cites W2141531658 @default.
• W4386319802 cites W2144179854 @default.
• W4386319802 cites W2145410423 @default.
• W4386319802 cites W2146305131 @default.
• W4386319802 cites W2151869895 @default.
• W4386319802 cites W2152724821 @default.
• W4386319802 cites W2154792548 @default.
• W4386319802 cites W2157820793 @default.
• W4386319802 cites W2162804111 @default.
• W4386319802 cites W2167042104 @default.
• W4386319802 cites W2270901278 @default.
• W4386319802 cites W2549468083 @default.
• W4386319802 cites W2566273359 @default.
• W4386319802 cites W2952907530 @default.
• W4386319802 cites W3106471163 @default.
• W4386319802 cites W3179832482 @default.
• W4386319802 cites W4506356 @default.
• W4386319802 cites W70790455 @default.

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