Matches in SemOpenAlex for { <https://semopenalex.org/work/W4386329342> ?p ?o ?g. }
- W4386329342 abstract "Abstract Pollen grains attached to insects are a valuable source of ecological information which can be used to reconstruct visitation networks. Morphological pollen identification relies on light microscopy with pollen usually stained and mounted in fuchsin jelly, which is also used to remove pollen from the bodies of insects. Pollen embedded in fuchsin jelly could potentially be used for DNA barcoding and metabarcoding (large‐scale taxonomic identification of complex mixed samples) and thus provide additional information for pollination networks. In this study, we determine whether fuchsin‐embedded pollen can be used for downstream molecular applications. We evaluate the quality of plant barcode (ITS) sequences amplified from DNA extracted from both fresh (untreated) pollen, and pollen which had been embedded in fuchsin jelly. We show that the addition of fuchsin to DNA extraction does not impact DNA barcode sequence quality during short‐term storage. DNA extractions from both untreated and fuchsin‐treated pollen produced reliable barcode sequences of high quality. Our findings suggest that pollen which has been collected, stained, and embedded in fuchsin jelly for preliminary microscopy work can be used within several days for downstream genetic analysis, though the quality of DNA from pollen stored in fuchsin jelly for extended periods is yet to be established." @default.
- W4386329342 created "2023-09-01" @default.
- W4386329342 creator A5015130891 @default.
- W4386329342 creator A5021753471 @default.
- W4386329342 creator A5031253725 @default.
- W4386329342 date "2023-08-31" @default.
- W4386329342 modified "2023-09-26" @default.
- W4386329342 title "Effect of fuchsin fixation of pollen on <scp>DNA</scp> barcode recovery" @default.
- W4386329342 cites W1837163102 @default.
- W4386329342 cites W1969042907 @default.
- W4386329342 cites W1975213815 @default.
- W4386329342 cites W1975455902 @default.
- W4386329342 cites W1976670550 @default.
- W4386329342 cites W1979146838 @default.
- W4386329342 cites W1981747359 @default.
- W4386329342 cites W1990282786 @default.
- W4386329342 cites W1990589634 @default.
- W4386329342 cites W2020334025 @default.
- W4386329342 cites W2021950521 @default.
- W4386329342 cites W2028858659 @default.
- W4386329342 cites W2045349678 @default.
- W4386329342 cites W2060329218 @default.
- W4386329342 cites W2088170018 @default.
- W4386329342 cites W2089346588 @default.
- W4386329342 cites W2113773347 @default.
- W4386329342 cites W2119923823 @default.
- W4386329342 cites W2121016876 @default.
- W4386329342 cites W2127556561 @default.
- W4386329342 cites W2127860913 @default.
- W4386329342 cites W2128880918 @default.
- W4386329342 cites W2132632499 @default.
- W4386329342 cites W2132926880 @default.
- W4386329342 cites W2133385595 @default.
- W4386329342 cites W2142359658 @default.
- W4386329342 cites W2146911076 @default.
- W4386329342 cites W2153474712 @default.
- W4386329342 cites W2197646827 @default.
- W4386329342 cites W2205059992 @default.
- W4386329342 cites W2313538252 @default.
- W4386329342 cites W2337603118 @default.
- W4386329342 cites W2623549608 @default.
- W4386329342 cites W2630465890 @default.
- W4386329342 cites W2756055130 @default.
- W4386329342 cites W2768563854 @default.
- W4386329342 cites W2811350630 @default.
- W4386329342 cites W2899957082 @default.
- W4386329342 cites W3015534386 @default.
- W4386329342 cites W3025280283 @default.
- W4386329342 cites W3135526626 @default.
- W4386329342 cites W3155428209 @default.
- W4386329342 cites W3157147776 @default.
- W4386329342 cites W3192094229 @default.
- W4386329342 cites W3199776145 @default.
- W4386329342 cites W4242635707 @default.
- W4386329342 doi "https://doi.org/10.1002/ece3.10475" @default.
- W4386329342 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/37664513" @default.
- W4386329342 hasPublicationYear "2023" @default.
- W4386329342 type Work @default.
- W4386329342 citedByCount "0" @default.
- W4386329342 crossrefType "journal-article" @default.
- W4386329342 hasAuthorship W4386329342A5015130891 @default.
- W4386329342 hasAuthorship W4386329342A5021753471 @default.
- W4386329342 hasAuthorship W4386329342A5031253725 @default.
- W4386329342 hasBestOaLocation W43863293421 @default.
- W4386329342 hasConcept C104317684 @default.
- W4386329342 hasConcept C111919701 @default.
- W4386329342 hasConcept C141732470 @default.
- W4386329342 hasConcept C2776841711 @default.
- W4386329342 hasConcept C2780618852 @default.
- W4386329342 hasConcept C41008148 @default.
- W4386329342 hasConcept C49105822 @default.
- W4386329342 hasConcept C552990157 @default.
- W4386329342 hasConcept C55493867 @default.
- W4386329342 hasConcept C59822182 @default.
- W4386329342 hasConcept C78063203 @default.
- W4386329342 hasConcept C86803240 @default.
- W4386329342 hasConcept C90856448 @default.
- W4386329342 hasConceptScore W4386329342C104317684 @default.
- W4386329342 hasConceptScore W4386329342C111919701 @default.
- W4386329342 hasConceptScore W4386329342C141732470 @default.
- W4386329342 hasConceptScore W4386329342C2776841711 @default.
- W4386329342 hasConceptScore W4386329342C2780618852 @default.
- W4386329342 hasConceptScore W4386329342C41008148 @default.
- W4386329342 hasConceptScore W4386329342C49105822 @default.
- W4386329342 hasConceptScore W4386329342C552990157 @default.
- W4386329342 hasConceptScore W4386329342C55493867 @default.
- W4386329342 hasConceptScore W4386329342C59822182 @default.
- W4386329342 hasConceptScore W4386329342C78063203 @default.
- W4386329342 hasConceptScore W4386329342C86803240 @default.
- W4386329342 hasConceptScore W4386329342C90856448 @default.
- W4386329342 hasIssue "9" @default.
- W4386329342 hasLocation W43863293421 @default.
- W4386329342 hasLocation W43863293422 @default.
- W4386329342 hasOpenAccess W4386329342 @default.
- W4386329342 hasPrimaryLocation W43863293421 @default.
- W4386329342 hasRelatedWork W1895143607 @default.
- W4386329342 hasRelatedWork W1991774093 @default.
- W4386329342 hasRelatedWork W2122184569 @default.
- W4386329342 hasRelatedWork W2383620395 @default.
- W4386329342 hasRelatedWork W2918727456 @default.