FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4386362054> ?p ?o ?g. }

• W4386362054 endingPage "e0290309" @default.
• W4386362054 startingPage "e0290309" @default.
• W4386362054 abstract "Ovine gammaherpesvirus 2 (OvHV-2), a member of the genus Macavirus, causes sheep-associated malignant catarrhal fever (SA-MCF), a fatal lymphoproliferative disease affecting a wide variety of ungulates in addition to horses. This study described an outbreak of SA-MCF in Mexico and the identification of the OvHV-2 virus in primary rabbit testis cultures through the generation of intranuclear inclusion bodies, syncytia, immunofluorescence (IF), immunocytochemistry (ICC), immunohistochemistry (IHC), endpoint polymerase chain reaction (PCR), and partial sequencing of the ORF75 gene. The animals involved in this outbreak showed mucogingival ulcers in the vestibule of the mouth and tongue, hypersalivation, corneal opacity, reduced food consumption, and weight loss of variable severity. These clinical signs and the histopathological findings suggested the diagnosis of SA-MCF. Buffy coat fractions from the anticoagulated blood samples of ill animals were collected and analyzed by PCR. Positive buffy coats were used to inoculate the primary cell cultures of rabbit testis to identify the virus. Small clusters of refractile cytomegalic cells, characteristic of viral cytopathic effects, were observed between 48 and 72 h post-infection. Furthermore, intranuclear acidophilic inclusion bodies (IBs) were identified in the inoculated primary culture cells, and the cytoplasm showed immunoreactivity with hyperimmune rabbit serum against OvHV-2. Moreover, in the liver histological sections from sick deer, immunoreactive juxtanuclear IBs were identified with the same rabbit hyperimmune serum. The obtained sequences were aligned with the OvHV-2 sequences reported in GenBank and revealed a nucleotide identity higher than 98%. Based on the evidence provided in this study, we conclude that the outbreak of SA-MCF in the municipality of Tequisquiapan in the state of Queretaro, Mexico, was caused by OvHV-2. This is the second study reporting that horses are susceptible to OvHV-2 infection and can develop SA-MCF. We identified for the first time in Mexico, the presence of OvHV-2 in buffy coats from horses and Artiodactyla." @default.
• W4386362054 created "2023-09-02" @default.
• W4386362054 creator A5011435230 @default.
• W4386362054 creator A5018135331 @default.
• W4386362054 creator A5018307527 @default.
• W4386362054 creator A5024563003 @default.
• W4386362054 creator A5072035902 @default.
• W4386362054 creator A5075361956 @default.
• W4386362054 creator A5075440125 @default.
• W4386362054 creator A5078514449 @default.
• W4386362054 creator A5086339366 @default.
• W4386362054 date "2023-09-01" @default.
• W4386362054 modified "2023-10-01" @default.
• W4386362054 title "First identification and characterization of ovine gammaherpesvirus type 2 in horses and artiodactyla from an outbreak of malignant catarrhal fever in Mexico" @default.
• W4386362054 cites W1669314850 @default.
• W4386362054 cites W1917133909 @default.
• W4386362054 cites W1993929220 @default.
• W4386362054 cites W1994370350 @default.
• W4386362054 cites W2034380977 @default.
• W4386362054 cites W2042971161 @default.
• W4386362054 cites W2050204382 @default.
• W4386362054 cites W2056547706 @default.
• W4386362054 cites W2061786849 @default.
• W4386362054 cites W2063097719 @default.
• W4386362054 cites W2063333623 @default.
• W4386362054 cites W2068180652 @default.
• W4386362054 cites W2087804175 @default.
• W4386362054 cites W2089327561 @default.
• W4386362054 cites W2096316506 @default.
• W4386362054 cites W2106492291 @default.
• W4386362054 cites W2114512485 @default.
• W4386362054 cites W2120611093 @default.
• W4386362054 cites W2124852401 @default.
• W4386362054 cites W2133345212 @default.
• W4386362054 cites W2143085905 @default.
• W4386362054 cites W2144775551 @default.
• W4386362054 cites W2146341019 @default.
• W4386362054 cites W2148685691 @default.
• W4386362054 cites W2152207030 @default.
• W4386362054 cites W2157614824 @default.
• W4386362054 cites W2161257356 @default.
• W4386362054 cites W2170824632 @default.
• W4386362054 cites W2256756668 @default.
• W4386362054 cites W2319797631 @default.
• W4386362054 cites W2400975186 @default.
• W4386362054 cites W2410256140 @default.
• W4386362054 cites W2416933228 @default.
• W4386362054 cites W2443556670 @default.
• W4386362054 cites W2474537816 @default.
• W4386362054 cites W2567168444 @default.
• W4386362054 cites W2567372311 @default.
• W4386362054 cites W2569425398 @default.
• W4386362054 cites W2586621986 @default.
• W4386362054 cites W2810517770 @default.
• W4386362054 cites W2904476400 @default.
• W4386362054 cites W3035707135 @default.
• W4386362054 cites W315748 @default.
• W4386362054 cites W3212230388 @default.
• W4386362054 cites W421608084 @default.
• W4386362054 cites W4251751280 @default.
• W4386362054 doi "https://doi.org/10.1371/journal.pone.0290309" @default.
• W4386362054 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/37656696" @default.
• W4386362054 hasPublicationYear "2023" @default.
• W4386362054 type Work @default.
• W4386362054 citedByCount "0" @default.
• W4386362054 crossrefType "journal-article" @default.
• W4386362054 hasAuthorship W4386362054A5011435230 @default.
• W4386362054 hasAuthorship W4386362054A5018135331 @default.
• W4386362054 hasAuthorship W4386362054A5018307527 @default.
• W4386362054 hasAuthorship W4386362054A5024563003 @default.
• W4386362054 hasAuthorship W4386362054A5072035902 @default.
• W4386362054 hasAuthorship W4386362054A5075361956 @default.
• W4386362054 hasAuthorship W4386362054A5075440125 @default.
• W4386362054 hasAuthorship W4386362054A5078514449 @default.
• W4386362054 hasAuthorship W4386362054A5086339366 @default.
• W4386362054 hasBestOaLocation W43863620541 @default.
• W4386362054 hasConcept C116675565 @default.
• W4386362054 hasConcept C142724271 @default.
• W4386362054 hasConcept C159047783 @default.
• W4386362054 hasConcept C203014093 @default.
• W4386362054 hasConcept C2522874641 @default.
• W4386362054 hasConcept C2778285947 @default.
• W4386362054 hasConcept C71924100 @default.
• W4386362054 hasConcept C86803240 @default.
• W4386362054 hasConceptScore W4386362054C116675565 @default.
• W4386362054 hasConceptScore W4386362054C142724271 @default.
• W4386362054 hasConceptScore W4386362054C159047783 @default.
• W4386362054 hasConceptScore W4386362054C203014093 @default.
• W4386362054 hasConceptScore W4386362054C2522874641 @default.
• W4386362054 hasConceptScore W4386362054C2778285947 @default.
• W4386362054 hasConceptScore W4386362054C71924100 @default.
• W4386362054 hasConceptScore W4386362054C86803240 @default.
• W4386362054 hasIssue "9" @default.
• W4386362054 hasLocation W43863620541 @default.
• W4386362054 hasLocation W43863620542 @default.
• W4386362054 hasOpenAccess W4386362054 @default.
• W4386362054 hasPrimaryLocation W43863620541 @default.
• W4386362054 hasRelatedWork W2016551366 @default.

• next