FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4386370259> ?p ?o ?g. }

• W4386370259 endingPage "1356" @default.
• W4386370259 startingPage "1347" @default.
• W4386370259 abstract "Human organic anion transporting polypeptide 2B1 (OATP2B1) is a membrane transporter widely expressed in organs crucial for drug absorption and disposition such as the intestine, liver, and kidney. Evidence indicates that OATP2B1 is a glycoprotein. However, the sites of glycosylation and their contribution to the function and expression of OATP2B1 are largely unknown. In this study, by site-directed mutagenesis, we determined that two of four potential N-glycosylation sites in OATP2B1, N176 and N538, are indeed glycosylated. Functional studies revealed that the transport activities of mutants N176Q and N538Q were greatly reduced as compared to that of wild-type OATP2B1. However, the reduced activity was not due to the impairment of transport function per se but due to the decreased surface expression as the Km and normalized Vmax values of N176Q and N538Q were comparable to those of OATP2B1. Quantitative polymerase chain reaction (PCR) revealed that N176Q and N538Q mutations did not affect the expression of OATP2B1 at a transcriptional level. Immunofluorescence analysis showed that deglycosylated OATP2B1 was largely retained in the endoplasmic reticulum, which may activate the endoplasmic reticulum-associated degradation pathway, and the ubiquitin–proteasome system played a major role in the degradation of OATP2B1. Taken together, OATP2B1 is N-glycosylated, and N-glycosylation is essential for the surface expression of OATP2B1 but not critical for the transport function of OATP2B1 per se." @default.
• W4386370259 created "2023-09-02" @default.
• W4386370259 creator A5004111700 @default.
• W4386370259 creator A5005896076 @default.
• W4386370259 creator A5020579044 @default.
• W4386370259 creator A5026191548 @default.
• W4386370259 creator A5036381691 @default.
• W4386370259 creator A5044192161 @default.
• W4386370259 creator A5060002817 @default.
• W4386370259 creator A5067057241 @default.
• W4386370259 creator A5068314399 @default.
• W4386370259 creator A5084846204 @default.
• W4386370259 creator A5086949663 @default.
• W4386370259 date "2023-09-01" @default.
• W4386370259 modified "2023-10-15" @default.
• W4386370259 title "Importance of N-Glycosylation for the Expression and Function of Human Organic Anion Transporting Polypeptide 2B1" @default.
• W4386370259 cites W1550259003 @default.
• W4386370259 cites W1565580371 @default.
• W4386370259 cites W1590553747 @default.
• W4386370259 cites W1647833960 @default.
• W4386370259 cites W1932475171 @default.
• W4386370259 cites W1957718603 @default.
• W4386370259 cites W1974501072 @default.
• W4386370259 cites W2017264771 @default.
• W4386370259 cites W2017940034 @default.
• W4386370259 cites W2024191848 @default.
• W4386370259 cites W2026231715 @default.
• W4386370259 cites W2028161249 @default.
• W4386370259 cites W2049122170 @default.
• W4386370259 cites W2051417300 @default.
• W4386370259 cites W2079748137 @default.
• W4386370259 cites W2083116825 @default.
• W4386370259 cites W2084827532 @default.
• W4386370259 cites W2088456241 @default.
• W4386370259 cites W2107277218 @default.
• W4386370259 cites W2115093402 @default.
• W4386370259 cites W2121335499 @default.
• W4386370259 cites W2122732537 @default.
• W4386370259 cites W2145006425 @default.
• W4386370259 cites W2148820240 @default.
• W4386370259 cites W2154679096 @default.
• W4386370259 cites W2155612332 @default.
• W4386370259 cites W2167450468 @default.
• W4386370259 cites W2206746709 @default.
• W4386370259 cites W2512565047 @default.
• W4386370259 cites W2573697958 @default.
• W4386370259 cites W2582336434 @default.
• W4386370259 cites W2811095894 @default.
• W4386370259 cites W2811301994 @default.
• W4386370259 cites W2900206619 @default.
• W4386370259 cites W2917456459 @default.
• W4386370259 cites W2945736670 @default.
• W4386370259 cites W2951978914 @default.
• W4386370259 cites W2966506307 @default.
• W4386370259 cites W2979839169 @default.
• W4386370259 cites W2984076969 @default.
• W4386370259 cites W3007227522 @default.
• W4386370259 cites W3084029311 @default.
• W4386370259 cites W3091815286 @default.
• W4386370259 cites W3119104707 @default.
• W4386370259 cites W3139366518 @default.
• W4386370259 cites W3143888301 @default.
• W4386370259 cites W3152110994 @default.
• W4386370259 cites W3199635621 @default.
• W4386370259 cites W3217549866 @default.
• W4386370259 cites W4225533659 @default.
• W4386370259 cites W4239399450 @default.
• W4386370259 cites W4281296922 @default.
• W4386370259 cites W4317936455 @default.
• W4386370259 doi "https://doi.org/10.1021/acsptsci.3c00076" @default.
• W4386370259 hasPublicationYear "2023" @default.
• W4386370259 type Work @default.
• W4386370259 citedByCount "0" @default.
• W4386370259 crossrefType "journal-article" @default.
• W4386370259 hasAuthorship W4386370259A5004111700 @default.
• W4386370259 hasAuthorship W4386370259A5005896076 @default.
• W4386370259 hasAuthorship W4386370259A5020579044 @default.
• W4386370259 hasAuthorship W4386370259A5026191548 @default.
• W4386370259 hasAuthorship W4386370259A5036381691 @default.
• W4386370259 hasAuthorship W4386370259A5044192161 @default.
• W4386370259 hasAuthorship W4386370259A5060002817 @default.
• W4386370259 hasAuthorship W4386370259A5067057241 @default.
• W4386370259 hasAuthorship W4386370259A5068314399 @default.
• W4386370259 hasAuthorship W4386370259A5084846204 @default.
• W4386370259 hasAuthorship W4386370259A5086949663 @default.
• W4386370259 hasConcept C104317684 @default.
• W4386370259 hasConcept C108625454 @default.
• W4386370259 hasConcept C120405084 @default.
• W4386370259 hasConcept C14036430 @default.
• W4386370259 hasConcept C143065580 @default.
• W4386370259 hasConcept C149011108 @default.
• W4386370259 hasConcept C153911025 @default.
• W4386370259 hasConcept C158617107 @default.
• W4386370259 hasConcept C185592680 @default.
• W4386370259 hasConcept C206212055 @default.
• W4386370259 hasConcept C2776678969 @default.
• W4386370259 hasConcept C2777313579 @default.
• W4386370259 hasConcept C30324644 @default.

• next