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- W4386407712 abstract "A detailed study of the cellular surfaceome poses major challenges for mass spectrometry analysis. Surface proteins are low abundant compared to intracellular proteins, and their inefficient extraction in aqueous medium leads to their aggregation and precipitation. To tackle such problems, surface biotinylation is frequently used to tag surface proteins with biotin, allowing for their enrichment, leading to a more sensitive mapping of surface proteomes. We here detail a new surface biotinylation protocol based on furan-biotin affinity purification to enrich plasma membrane proteins for proteomics. This protocol involves biotinylation of cell surface membrane proteins on viable cells, followed by affinity enrichment using streptavidin beads, trypsin digestion, peptide cleanup, and LC-MS/MS analysis." @default.
- W4386407712 created "2023-09-05" @default.
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- W4386407712 date "2023-01-01" @default.
- W4386407712 modified "2023-09-30" @default.
- W4386407712 title "Cell Surface Biotinylation Using Furan Cross-Linking Chemistry" @default.
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- W4386407712 doi "https://doi.org/10.1007/978-1-0716-3457-8_2" @default.
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