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- W4386407934 abstract "Mass spectrometry (MS)-based proteomics is a rapidly maturing discipline, thus gaining momentum for routine molecular profiling of clinical specimens to improve disease classification, diagnostics, and therapy development. Yet, hurdles need to be overcome to enhance reproducibility in preanalytical sample processing, especially in large, quantity-limited sample cohorts. Therefore, automated sonication and single-pot solid-phase-enhanced sample preparation (autoSP3) was developed as a streamlined workflow that integrates all tasks from tissue lysis and protein extraction, protein cleanup, and proteolysis. It enables the concurrent processing of 96 clinical samples of any type (fresh-frozen or FFPE tissue, liquid biopsies, or cells) on an automated liquid handling platform, which can be directly interfaced to LC–MS for proteome analysis of clinical specimens with high sensitivity, high reproducibility, and short turn-around times." @default.
- W4386407934 created "2023-09-05" @default.
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- W4386407934 date "2023-01-01" @default.
- W4386407934 modified "2023-09-30" @default.
- W4386407934 title "Automated Sample Preparation for Mass Spectrometry-Based Clinical Proteomics" @default.
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- W4386407934 doi "https://doi.org/10.1007/978-1-0716-3457-8_11" @default.
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