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• W4386408048 abstract "Terminal amine isotopic labeling of substrates (TAILS) is a sensitive and robust quantitative mass spectrometry (MS)-based proteomics method used for the characterization of physiological or proteolytically processed protein N-termini, as well as other N-terminal posttranslational modifications (PTMs). TAILS is a well-established, high-throughput, negative enrichment workflow that enables system-wide exploration of N-terminomes independent of sample complexity. TAILS makes use of amine reactivity of free N-termini and a highly efficient aldehyde-functionalized polymer to deplete internal peptides generated after proteolytic digestion during sample preparation. Thereby, it enriches for natural N-termini, allowing for unbiased and complete investigation of differential proteolysis, protease substrate discovery, and analysis of N-terminal PTMs. In this chapter, we provide a state-of-the-art protocol, with detailed steps in all parts of the TAILS sample preparation, MS analysis, and post-processing of acquired data." @default.
• W4386408048 created "2023-09-05" @default.
• W4386408048 creator A5021841289 @default.
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• W4386408048 date "2023-01-01" @default.
• W4386408048 modified "2023-09-30" @default.
• W4386408048 title "Sensitive and High-Throughput Exploration of Protein N-Termini by TMT-TAILS N-Terminomics" @default.
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• W4386408048 doi "https://doi.org/10.1007/978-1-0716-3457-8_7" @default.
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