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- W4386415222 abstract "Despite being the most popular, cow's milk and dairy products are one of the major allergens for infant, young children, and adults. We developed and validated a daPCR-CHA-FB assay to detect cow DNA in milk and dairy products. During optimization, we obtained a linear relationship between synthetic cow DNA and fluorescence signal (y = 0.0437x + 0.7966; r2 = 0.9814) and demonstrated that the assay could be used without any sample preparation. An accuracy of 100% was obtained with 50 vouchered cow's milk samples. No cross-reactivity was observed with nine other species. The detection limit was 1x10−4 ng of DNA. It is also highly robust, as demonstrated with almost 250 market samples (observed fraud ratio of 15.4%). The assay could be completed in less than 2 h, does not require any DNA purification and expensive equipment such as a real-time thermal cycler, or a capillary electrophoresis." @default.
- W4386415222 created "2023-09-05" @default.
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- W4386415222 date "2024-01-01" @default.
- W4386415222 modified "2023-10-16" @default.
- W4386415222 title "A novel biosensor method to detect cow DNA in milk and dairy products" @default.
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- W4386415222 doi "https://doi.org/10.1016/j.foodcont.2023.110096" @default.
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