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- W4386437907 abstract "CRISPR-Cas9 is a powerful tool to interrogate gene function in a targeted and systematic manner. Although the technology has been scaled up for large-scale genetic screens in cell culture, similar scale screens in vivo have been extremely challenging due to the cost, labor, and time required to generate and keep track of thousands of mutant animals. We reported the development of Multiplexed Intermixed CRISPR Droplets (MIC-Drop), a platform that makes large-scale reverse genetic screens possible in zebrafish. In this chapter, we provide a detailed protocol to conduct large-scale genetic screens using this novel platform." @default.
- W4386437907 created "2023-09-06" @default.
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- W4386437907 date "2023-09-06" @default.
- W4386437907 modified "2023-09-30" @default.
- W4386437907 title "Scalable CRISPR Screens in Zebrafish Using MIC-Drop" @default.
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- W4386437907 doi "https://doi.org/10.1007/978-1-0716-3401-1_21" @default.
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