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- W4386446569 endingPage "e19429" @default.
- W4386446569 startingPage "e19429" @default.
- W4386446569 abstract "Fe3O4@SiO2@NiAl-LDH three-components microsphere contains a Fe3O4@SiO2 magnetic core and a layered double hydroxide with nickel cation provide the binding ability to (His)-tagged-protein and exhibits high performance in protein separation and purification. The morphology and chemistry of the synthesized Fe3O4@SiO2@NiAl-LDH microspheres were characterized by energy-dispersive X-ray spectroscopy (EDX), scanning electron microscopy (SEM), X-ray diffraction (XRD), Fourier transform infrared (FTIR), vibrating sample magnetometer (VSM), Dynamic light scattering (DLS). Purified enzyme was assesed with SDS-PAGE (sodium dodecyl sulfate-polyacrylamide gel electrophoresis and intrinsic fluorescence spectroscopy. In this study, the separation of phenylalanine dehydrogenase (PheDH) by Fe3O4@SiO2@NiAl -LDH was performed and the effect of microsphere was investigated on the kinetic and structural properties of PheDH. After purification, kinetic parameters such as Km, Vmax, Kcat, kcat/Km, optimum temperature, thermal stability, and and activation energy were evaluated and compared according to the mentioned methods. The interaction between the enzyme and the microsphere displayed a high performance in protein binding capacity. The results also revealed that the kinetic parameters of the enzyme changed in a dose-dependent manner in the presence of a microsphere. Moreover, the results of intrinsic fluorescence and Circular Dichroism (CD) confirmed the structural changes of the protein in the interaction with the microsphere." @default.
- W4386446569 created "2023-09-06" @default.
- W4386446569 creator A5002216274 @default.
- W4386446569 creator A5029964894 @default.
- W4386446569 creator A5032181881 @default.
- W4386446569 creator A5072273516 @default.
- W4386446569 creator A5092150230 @default.
- W4386446569 date "2023-09-01" @default.
- W4386446569 modified "2023-10-16" @default.
- W4386446569 title "Fe3O4@SiO2@NiAl-LDH microspheres implication in separation, kinetic and structural properties of phenylalanine dehydrogenase" @default.
- W4386446569 cites W1210912314 @default.
- W4386446569 cites W1724284269 @default.
- W4386446569 cites W1763015095 @default.
- W4386446569 cites W1915707355 @default.
- W4386446569 cites W1977818632 @default.
- W4386446569 cites W1979338300 @default.
- W4386446569 cites W1979511569 @default.
- W4386446569 cites W1983556727 @default.
- W4386446569 cites W1988296646 @default.
- W4386446569 cites W1992514216 @default.
- W4386446569 cites W1999540659 @default.
- W4386446569 cites W2001001421 @default.
- W4386446569 cites W2009180810 @default.
- W4386446569 cites W2010321179 @default.
- W4386446569 cites W2011111272 @default.
- W4386446569 cites W2011209568 @default.
- W4386446569 cites W2012785401 @default.
- W4386446569 cites W2015551764 @default.
- W4386446569 cites W2020892651 @default.
- W4386446569 cites W2021443929 @default.
- W4386446569 cites W2021871707 @default.
- W4386446569 cites W2024869871 @default.
- W4386446569 cites W2026138877 @default.
- W4386446569 cites W2030652025 @default.
- W4386446569 cites W2034164480 @default.
- W4386446569 cites W2035255891 @default.
- W4386446569 cites W2039068652 @default.
- W4386446569 cites W2040864301 @default.
- W4386446569 cites W2043508378 @default.
- W4386446569 cites W2046773363 @default.
- W4386446569 cites W2048026992 @default.
- W4386446569 cites W2050524601 @default.
- W4386446569 cites W2062947943 @default.
- W4386446569 cites W2069682319 @default.
- W4386446569 cites W2073732456 @default.
- W4386446569 cites W2073803381 @default.
- W4386446569 cites W2076335856 @default.
- W4386446569 cites W2084786716 @default.
- W4386446569 cites W2084871302 @default.
- W4386446569 cites W2086030168 @default.
- W4386446569 cites W2086992393 @default.
- W4386446569 cites W2091584390 @default.
- W4386446569 cites W2091950532 @default.
- W4386446569 cites W2093816221 @default.
- W4386446569 cites W2095241233 @default.
- W4386446569 cites W2109795543 @default.
- W4386446569 cites W2122046010 @default.
- W4386446569 cites W2143208806 @default.
- W4386446569 cites W2145563967 @default.
- W4386446569 cites W2149110232 @default.
- W4386446569 cites W2158558808 @default.
- W4386446569 cites W2161802938 @default.
- W4386446569 cites W2221915025 @default.
- W4386446569 cites W2300804555 @default.
- W4386446569 cites W2322768231 @default.
- W4386446569 cites W2325029026 @default.
- W4386446569 cites W2327979313 @default.
- W4386446569 cites W2507461661 @default.
- W4386446569 cites W2533894695 @default.
- W4386446569 cites W2556263817 @default.
- W4386446569 cites W2560131631 @default.
- W4386446569 cites W2563044322 @default.
- W4386446569 cites W2579982376 @default.
- W4386446569 cites W2589016571 @default.
- W4386446569 cites W2591910953 @default.
- W4386446569 cites W2739668013 @default.
- W4386446569 cites W2765742852 @default.
- W4386446569 cites W2768471315 @default.
- W4386446569 cites W2768588919 @default.
- W4386446569 cites W2783468095 @default.
- W4386446569 cites W2790211976 @default.
- W4386446569 cites W2793815130 @default.
- W4386446569 cites W2800102334 @default.
- W4386446569 cites W2802907897 @default.
- W4386446569 cites W2804520439 @default.
- W4386446569 cites W2896251180 @default.
- W4386446569 cites W2896571979 @default.
- W4386446569 cites W2898621778 @default.
- W4386446569 cites W2901985518 @default.
- W4386446569 cites W2910884576 @default.
- W4386446569 cites W2911997172 @default.
- W4386446569 cites W2912411743 @default.
- W4386446569 cites W2913729364 @default.
- W4386446569 cites W2915861604 @default.
- W4386446569 cites W2939868378 @default.
- W4386446569 cites W2946342926 @default.
- W4386446569 cites W2973197677 @default.
- W4386446569 cites W2980709183 @default.