Matches in SemOpenAlex for { <https://semopenalex.org/work/W4386460049> ?p ?o ?g. }
- W4386460049 abstract "ABSTRACT Following the encounter with an unrepaired DNA lesion, replication is halted and can restart downstream of the lesion leading to the formation of a single-stranded DNA (ssDNA) gap. To complete replication, this ssDNA gap is filled in by one of the two known lesion tolerance pathways: the error-prone Translesion Synthesis (TLS) or the error-free Homology Directed Gap Repair (HDGR). In the present work, we show a role for the RecBC complex that is distinct from its canonical function in homologous recombination at double strand breaks: RecBC (independently of the RecD subunit) is required to protect the 3’-end of the nascent DNA at the lesion site, in order to promote efficient lesion bypass. In the absence of RecBC, the nuclease ExoI can access and degrade the nascent DNA, thus strongly inhibiting the action of the TLS DNA polymerases and also affecting the HDGR mechanism. RecBC is recruited only when post-replicatively ssDNA gaps are formed and are covered by the single-stranded binding protein (SSB). This protective role of RecBC is reminiscent of BRCA2’s role in human cells, once again highlighting the evolutionary conservation of DNA replication mechanisms across all living organisms." @default.
- W4386460049 created "2023-09-06" @default.
- W4386460049 creator A5009886537 @default.
- W4386460049 creator A5025743993 @default.
- W4386460049 creator A5035334632 @default.
- W4386460049 creator A5057384771 @default.
- W4386460049 creator A5058213120 @default.
- W4386460049 creator A5060250868 @default.
- W4386460049 date "2023-09-04" @default.
- W4386460049 modified "2023-09-27" @default.
- W4386460049 title "The RecBC complex protects the nascent DNA in the presence of single-stranded DNA gaps" @default.
- W4386460049 cites W1481806672 @default.
- W4386460049 cites W1509550144 @default.
- W4386460049 cites W1544663300 @default.
- W4386460049 cites W1556563898 @default.
- W4386460049 cites W1600925677 @default.
- W4386460049 cites W1901739389 @default.
- W4386460049 cites W1913308298 @default.
- W4386460049 cites W1930393440 @default.
- W4386460049 cites W1960608041 @default.
- W4386460049 cites W1970578083 @default.
- W4386460049 cites W1975853779 @default.
- W4386460049 cites W1976713597 @default.
- W4386460049 cites W1987397407 @default.
- W4386460049 cites W1989108993 @default.
- W4386460049 cites W1991988366 @default.
- W4386460049 cites W1992657957 @default.
- W4386460049 cites W1992880065 @default.
- W4386460049 cites W1994775292 @default.
- W4386460049 cites W2000709611 @default.
- W4386460049 cites W2000826427 @default.
- W4386460049 cites W2005402849 @default.
- W4386460049 cites W2014750558 @default.
- W4386460049 cites W2018841459 @default.
- W4386460049 cites W2024922257 @default.
- W4386460049 cites W2025007049 @default.
- W4386460049 cites W2025248053 @default.
- W4386460049 cites W2026133647 @default.
- W4386460049 cites W2042690998 @default.
- W4386460049 cites W2044375045 @default.
- W4386460049 cites W2051943436 @default.
- W4386460049 cites W2052109832 @default.
- W4386460049 cites W2056473684 @default.
- W4386460049 cites W2063335678 @default.
- W4386460049 cites W2064402458 @default.
- W4386460049 cites W2065184116 @default.
- W4386460049 cites W2065688789 @default.
- W4386460049 cites W2071692230 @default.
- W4386460049 cites W2078095931 @default.
- W4386460049 cites W2078898807 @default.
- W4386460049 cites W2079478900 @default.
- W4386460049 cites W2091386551 @default.
- W4386460049 cites W2092174747 @default.
- W4386460049 cites W2105904762 @default.
- W4386460049 cites W2111967267 @default.
- W4386460049 cites W2116137883 @default.
- W4386460049 cites W2124560745 @default.
- W4386460049 cites W2124762966 @default.
- W4386460049 cites W2127209192 @default.
- W4386460049 cites W2127858651 @default.
- W4386460049 cites W2128298547 @default.
- W4386460049 cites W2132413391 @default.
- W4386460049 cites W2133290100 @default.
- W4386460049 cites W2143669599 @default.
- W4386460049 cites W2144463188 @default.
- W4386460049 cites W2145304961 @default.
- W4386460049 cites W2145532900 @default.
- W4386460049 cites W2146533600 @default.
- W4386460049 cites W2147121776 @default.
- W4386460049 cites W2151541959 @default.
- W4386460049 cites W2154087633 @default.
- W4386460049 cites W2160072480 @default.
- W4386460049 cites W2165108940 @default.
- W4386460049 cites W2208662860 @default.
- W4386460049 cites W2292458301 @default.
- W4386460049 cites W2335724328 @default.
- W4386460049 cites W2518845021 @default.
- W4386460049 cites W2550762644 @default.
- W4386460049 cites W2577344516 @default.
- W4386460049 cites W2580692595 @default.
- W4386460049 cites W2616616891 @default.
- W4386460049 cites W2765730443 @default.
- W4386460049 cites W2775105440 @default.
- W4386460049 cites W2784728212 @default.
- W4386460049 cites W2794497310 @default.
- W4386460049 cites W2802813826 @default.
- W4386460049 cites W2903475730 @default.
- W4386460049 cites W2951380187 @default.
- W4386460049 cites W2953255197 @default.
- W4386460049 cites W2957110995 @default.
- W4386460049 cites W3127681463 @default.
- W4386460049 cites W3133985301 @default.
- W4386460049 cites W3165056417 @default.
- W4386460049 cites W4200124957 @default.
- W4386460049 cites W4282010062 @default.
- W4386460049 cites W4297249507 @default.
- W4386460049 cites W4300594378 @default.
- W4386460049 cites W81157748 @default.
- W4386460049 doi "https://doi.org/10.1101/2023.09.04.556180" @default.
- W4386460049 hasPublicationYear "2023" @default.