FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4386462017> ?p ?o ?g. }

• W4386462017 abstract "A dieback of apple trees (Malus domestica (Suckow) Borkh.) associated with cankers was observed in commercial orchards in southwestern Ontario, Canada, in 2019. Fifteen 2 to 10-year-old symptomatic trees were collected from three orchards exhibiting up to 37% disease incidence. Small sections of diseased wood (1 cm long) were surface sterilized with 70% ethanol for 30 sec and 1% NaClO for 20 min, rinsed thrice in sterile water, placed on 2% PDA (Difco) amended with kanamycin (50 mg liter−1), and incubated at 22°C for 5 days in the dark (Ilyukhin et al. 2023). Fungal colonies that were consistently isolated were hyphal-tipped, transferred to individual PDA plates and incubated at 22°C for 7 days in the dark. Purified isolates with same characteristics were classed into morphotypes. One morphotype was initially white and turned dark olivaceous with dense aerial mycelium. Pycnidia were produced on pine needles on PDA (Fig. S2) after incubation at 22°C for 17 days in the dark. Conidia were brown, aseptate, ovoid, and measured 27.9 to 31.3 μm x 12.1 to 14.2 μm (mean ± S.D. of 15 conidia = 29.9 ± 0.9 μm × 13.2 ± 0.6 μm), the typical morphology of a Diplodia sp. (Phillips et al. 2012). Genomic DNA was extracted from a 7-day-old culture of a representative isolate M45-28, using the Plant/Fungi DNA Isolation Kit (Norgen Biotech, Canada). The internal transcribed spacer (ITS), translation elongation factor 1-α (EF1-α) and β-tubulin gene regions were amplified and sequenced with primers ITS1/ITS4, EF1-728F/EF1-986R and Bt2a/Bt2b and deposited in GenBank with accession numbers MZ970605, MZ995430 and MZ995431, respectively. Based on the sequence, the fungus was identified as Diplodia intermedia A.J.L. Phillips et al. and matched isolates from different hosts and countries (ITS: 100%, MG220378; EF1-α: 100%, MG220385; β-tubulin: 99.24%, MT592502). The maximum likelihood-based phylogenetic analysis of ITS, EF1-α and β-tubulin concatenated sequences was performed using IQ-Tree 2.2.2.7 (Minh et al. 2020). M45-28 was clustered with high bootstrap support values with D. intermedia isolates from the Westerdijk Fungal Biodiversity Institute collection, including the ex-holotype (CBS 124462) (Fig. S1). A living culture of M45-28 was deposited in the Canadian Collection of Fungal Cultures (DAOMC 252253). Pathogenicity assay was conducted by inoculating mycelial plugs from a 7-day-old culture of M45-28 into wounds made on the trunk of 5 eight-month-old potted healthy ‘Royal Gala’ apple seedlings. Five control seedlings were inoculated with sterile plugs. Canker symptoms appeared 15 days after inoculation, spread around, up and down the main stem from the inoculation point, and by 7 weeks the upper portion of the seedling was dead (Fig. S2). Diplodia intermedia was re-isolated from all inoculated seedlings and species identity was confirmed by sequencing as described above, fulfilling Koch’s postulates. Control seedlings remained symptomless and the fungus was not isolated from the wood. Diplodia intermedia was reported to cause cankers on apple in Uruguay (Delgado-Cerrone et al. 2016), wild apple (Malus sylvestris) in Portugal (Phillips et al. 2012), grapevines in France (Comont et al. 2016) and forest trees in Iran (Kazemzadeh Chakusary et al. 2019). To the best of our knowledge, this is the first report of D. intermedia causing canker and dieback diseases on apple trees in Canada. Further studies are required to better understand the epidemiology involved in the dynamic spread of the disease in order to recommend an adequate phytosanitary program for its control." @default.
• W4386462017 created "2023-09-06" @default.
• W4386462017 creator A5004093328 @default.
• W4386462017 creator A5042709914 @default.
• W4386462017 creator A5048214471 @default.
• W4386462017 creator A5066005944 @default.
• W4386462017 date "2023-09-05" @default.
• W4386462017 modified "2023-10-01" @default.
• W4386462017 title "First Report of <i>Diplodia intermedia</i> Causing Canker and Dieback Diseases on Apple Trees in Canada" @default.
• W4386462017 doi "https://doi.org/10.1094/pdis-07-23-1361-pdn" @default.
• W4386462017 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/37669178" @default.
• W4386462017 hasPublicationYear "2023" @default.
• W4386462017 type Work @default.
• W4386462017 citedByCount "0" @default.
• W4386462017 crossrefType "journal-article" @default.
• W4386462017 hasAuthorship W4386462017A5004093328 @default.
• W4386462017 hasAuthorship W4386462017A5042709914 @default.
• W4386462017 hasAuthorship W4386462017A5048214471 @default.
• W4386462017 hasAuthorship W4386462017A5066005944 @default.
• W4386462017 hasConcept C133479454 @default.
• W4386462017 hasConcept C144027150 @default.
• W4386462017 hasConcept C174618031 @default.
• W4386462017 hasConcept C180032290 @default.
• W4386462017 hasConcept C183476254 @default.
• W4386462017 hasConcept C2777083264 @default.
• W4386462017 hasConcept C2777247689 @default.
• W4386462017 hasConcept C2777306635 @default.
• W4386462017 hasConcept C2781255879 @default.
• W4386462017 hasConcept C43143990 @default.
• W4386462017 hasConcept C59822182 @default.
• W4386462017 hasConcept C86803240 @default.
• W4386462017 hasConcept C90080823 @default.
• W4386462017 hasConceptScore W4386462017C133479454 @default.
• W4386462017 hasConceptScore W4386462017C144027150 @default.
• W4386462017 hasConceptScore W4386462017C174618031 @default.
• W4386462017 hasConceptScore W4386462017C180032290 @default.
• W4386462017 hasConceptScore W4386462017C183476254 @default.
• W4386462017 hasConceptScore W4386462017C2777083264 @default.
• W4386462017 hasConceptScore W4386462017C2777247689 @default.
• W4386462017 hasConceptScore W4386462017C2777306635 @default.
• W4386462017 hasConceptScore W4386462017C2781255879 @default.
• W4386462017 hasConceptScore W4386462017C43143990 @default.
• W4386462017 hasConceptScore W4386462017C59822182 @default.
• W4386462017 hasConceptScore W4386462017C86803240 @default.
• W4386462017 hasConceptScore W4386462017C90080823 @default.
• W4386462017 hasLocation W43864620171 @default.
• W4386462017 hasLocation W43864620172 @default.
• W4386462017 hasOpenAccess W4386462017 @default.
• W4386462017 hasPrimaryLocation W43864620171 @default.
• W4386462017 hasRelatedWork W1501393966 @default.
• W4386462017 hasRelatedWork W1981238315 @default.
• W4386462017 hasRelatedWork W2010689732 @default.
• W4386462017 hasRelatedWork W2032049327 @default.
• W4386462017 hasRelatedWork W2042610915 @default.
• W4386462017 hasRelatedWork W2295336910 @default.
• W4386462017 hasRelatedWork W2360361639 @default.
• W4386462017 hasRelatedWork W2982358531 @default.
• W4386462017 hasRelatedWork W3165830776 @default.
• W4386462017 hasRelatedWork W4242715908 @default.
• W4386462017 isParatext "false" @default.
• W4386462017 isRetracted "false" @default.
• W4386462017 workType "article" @default.