FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4386475160> ?p ?o ?g. }

• W4386475160 abstract "Abstract We report our findings on the assembly of the HIV-1 protein Vpu into soluble oligomers. Vpu is a key HIV-1 protein. It has been considered exclusively a single-pass membrane protein. Previous observations show that this protein forms stable oligomers in aqueous solution, but details about these oligomers still remain obscure. This is an interesting and rather unique observation, as the number of proteins transitioning between soluble and membrane embedded states is limited. In this study we made use of protein engineering, size exclusion chromatography, cryoEM and electron paramagnetic resonance (EPR) spectroscopy to better elucidate the nature of the soluble oligomers. We found that Vpu oligomerizes via its N-terminal transmembrane domain (TM). CryoEM suggests that the oligomeric state most likely is a hexamer/heptamer equilibrium. Both cryoEM and EPR suggest that, within the oligomer, the distal C-terminal region of Vpu is highly flexible. Our observations are consistent with both the concept of specific interactions among TM helices or the core of the oligomers being stabilized by hydrophobic forces. While this study does not resolve all of the questions about Vpu oligomers or their functional role in HIV-1 it provides new fundamental information about the size and nature of the oligomeric interactions." @default.
• W4386475160 created "2023-09-07" @default.
• W4386475160 creator A5006552242 @default.
• W4386475160 creator A5010730078 @default.
• W4386475160 creator A5043428416 @default.
• W4386475160 creator A5050658132 @default.
• W4386475160 creator A5053994968 @default.
• W4386475160 creator A5058057355 @default.
• W4386475160 creator A5063840639 @default.
• W4386475160 date "2023-09-06" @default.
• W4386475160 modified "2023-09-28" @default.
• W4386475160 title "HIV-1 Vpu protein forms stable oligomers in aqueous solution via its transmembrane domain self-association" @default.
• W4386475160 cites W1539918506 @default.
• W4386475160 cites W1555930389 @default.
• W4386475160 cites W1814017524 @default.
• W4386475160 cites W1824377481 @default.
• W4386475160 cites W1891596881 @default.
• W4386475160 cites W1953456383 @default.
• W4386475160 cites W1984125372 @default.
• W4386475160 cites W1998685200 @default.
• W4386475160 cites W2001362053 @default.
• W4386475160 cites W2001430278 @default.
• W4386475160 cites W2003756762 @default.
• W4386475160 cites W2010458160 @default.
• W4386475160 cites W2010715944 @default.
• W4386475160 cites W2014283693 @default.
• W4386475160 cites W2021067210 @default.
• W4386475160 cites W2033873142 @default.
• W4386475160 cites W2035867814 @default.
• W4386475160 cites W2045035889 @default.
• W4386475160 cites W2045684019 @default.
• W4386475160 cites W2058828092 @default.
• W4386475160 cites W2066589127 @default.
• W4386475160 cites W2071971425 @default.
• W4386475160 cites W2080056514 @default.
• W4386475160 cites W2086280683 @default.
• W4386475160 cites W2091646550 @default.
• W4386475160 cites W2098513990 @default.
• W4386475160 cites W2099164564 @default.
• W4386475160 cites W2100455255 @default.
• W4386475160 cites W2104234755 @default.
• W4386475160 cites W2112121524 @default.
• W4386475160 cites W2128326592 @default.
• W4386475160 cites W2133442602 @default.
• W4386475160 cites W2134193705 @default.
• W4386475160 cites W2139927157 @default.
• W4386475160 cites W2149258763 @default.
• W4386475160 cites W2155766451 @default.
• W4386475160 cites W2172012533 @default.
• W4386475160 cites W2198297310 @default.
• W4386475160 cites W2233751927 @default.
• W4386475160 cites W2278419621 @default.
• W4386475160 cites W2466139738 @default.
• W4386475160 cites W2493151343 @default.
• W4386475160 cites W2520930511 @default.
• W4386475160 cites W2565271894 @default.
• W4386475160 cites W2802055049 @default.
• W4386475160 cites W2883753771 @default.
• W4386475160 cites W2891338881 @default.
• W4386475160 cites W2911458485 @default.
• W4386475160 cites W2942918459 @default.
• W4386475160 cites W2970951657 @default.
• W4386475160 cites W3037635279 @default.
• W4386475160 cites W3137102412 @default.
• W4386475160 cites W3184072622 @default.
• W4386475160 cites W3184548922 @default.
• W4386475160 cites W4320731530 @default.
• W4386475160 doi "https://doi.org/10.1038/s41598-023-41873-0" @default.
• W4386475160 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/37673923" @default.
• W4386475160 hasPublicationYear "2023" @default.
• W4386475160 type Work @default.
• W4386475160 citedByCount "0" @default.
• W4386475160 crossrefType "journal-article" @default.
• W4386475160 hasAuthorship W4386475160A5006552242 @default.
• W4386475160 hasAuthorship W4386475160A5010730078 @default.
• W4386475160 hasAuthorship W4386475160A5043428416 @default.
• W4386475160 hasAuthorship W4386475160A5050658132 @default.
• W4386475160 hasAuthorship W4386475160A5053994968 @default.
• W4386475160 hasAuthorship W4386475160A5058057355 @default.
• W4386475160 hasAuthorship W4386475160A5063840639 @default.
• W4386475160 hasBestOaLocation W43864751601 @default.
• W4386475160 hasConcept C104317684 @default.
• W4386475160 hasConcept C118892022 @default.
• W4386475160 hasConcept C121332964 @default.
• W4386475160 hasConcept C12554922 @default.
• W4386475160 hasConcept C144292202 @default.
• W4386475160 hasConcept C144647389 @default.
• W4386475160 hasConcept C147789679 @default.
• W4386475160 hasConcept C161200384 @default.
• W4386475160 hasConcept C170493617 @default.
• W4386475160 hasConcept C184651966 @default.
• W4386475160 hasConcept C185592680 @default.
• W4386475160 hasConcept C187961010 @default.
• W4386475160 hasConcept C188027245 @default.
• W4386475160 hasConcept C189754071 @default.
• W4386475160 hasConcept C24530287 @default.
• W4386475160 hasConcept C2780642029 @default.
• W4386475160 hasConcept C41625074 @default.
• W4386475160 hasConcept C46141821 @default.
• W4386475160 hasConcept C47701112 @default.

• next