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- W4386475175 abstract "<p>Antibiotic eradication of <i>Hp</i> reverses metaplastic pit cell expansion. <i>Muc4</i> and gastric inflammation were assessed 12 weeks after KRAS induction in the following groups: <i>Hp</i>−KRAS+ mice; <i>Hp</i>+KRAS+ mice; and <i>Hp</i>+KRAS+ mice treated with antibiotics (“abx”) from weeks 6 to 8. <b>A,</b> ISH was used to detect <i>Muc4</i> (brown) in the corpus at 12 weeks and representative images are shown. Scale bars, 100 μm. <b>B–E,</b> IHC was used to detect the indicated immune cell populations in gastric corpus tissue at 12 weeks. Data are from <i>N</i> = 2 independent experiments with <i>n</i> = 5–10 mice per group. <b>B,</b> Representative images of the gastric corpus are shown. CD3 (yellow) indicates T cells, CD4 (red) indicates Th cells, CD8α (green) indicates cytotoxic T cells, F4/80 (gray) indicates macrophages and DAPI (blue) indicates nuclei. Higher magnification images of the boxed regions are shown in the second row. Scale bars, 100 μm. <b>C–E,</b> The indicated immune cell populations were detected by IHC and quantified in each mouse using HALO software in <i>N</i> = 2 experiments. Each datapoint is the average quantitation in two fields of view for an individual mouse and bars represent the medians. ***, <i>P</i> < 0.001; ****, <i>P</i> < 0.0001; Mann–Whitney <i>U</i> test; ns, not significant.</p>" @default.
- W4386475175 created "2023-09-07" @default.
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- W4386475175 date "2023-09-06" @default.
- W4386475175 modified "2023-09-29" @default.
- W4386475175 title "FIGURE 4 from Single-cell Profiling Uncovers a <i>Muc4</i>-Expressing Metaplastic Gastric Cell Type Sustained by <i>Helicobacter pylori</i>-driven Inflammation" @default.
- W4386475175 doi "https://doi.org/10.1158/2767-9764.24089962.v1" @default.
- W4386475175 hasPublicationYear "2023" @default.
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