Matches in SemOpenAlex for { <https://semopenalex.org/work/W4386475757> ?p ?o ?g. }
- W4386475757 endingPage "2826" @default.
- W4386475757 startingPage "2826" @default.
- W4386475757 abstract "The slow freezing of boar sperm is the only way to preserve genetic material for extended periods; this can be achieved with exposure to liquid nitrogen vapors (conventional) or by using automated freezing equipment. The aim was to compare the effect of both techniques on post-thaw functionality. Boar sperm devoid of seminal plasma and resuspended in lactose-egg yolk-glycerol medium were cryopreserved. Conventional: straws were exposed to LN2 vapors; automated: using a drop curve of -39.82 °C·min-1 for 113 s from -5 to -80 °C during the critical period; and subsequent immersion in NL2. Cell viability, cholesterol flow, mitochondrial membrane potential (MMP), lipid peroxidation, peroxynitrite, superoxide anion levels, phosphatidylserine translocation, and caspase activation were evaluated by flow cytometry. In addition, total motility (TM) and progressive motility (PM) were determined by the SCA system immediately (T0), 60 (T60), and 120 min (T120) post-thawing. Automated freezing significantly reduces cholesterol flow and free radical and lipid peroxidation levels, making it possible to preserve motility for 120 min of incubation. At the same time, viability, acrosome integrity, MMP, and caspase activation did not differ from the conventional technique. In conclusion, controlling the temperature drop curve using automated freezing equipment reduces oxidative/nitrosative stress, preserving membrane fluidity and sperm motility." @default.
- W4386475757 created "2023-09-07" @default.
- W4386475757 creator A5020033680 @default.
- W4386475757 creator A5026474041 @default.
- W4386475757 creator A5040024728 @default.
- W4386475757 creator A5052129391 @default.
- W4386475757 creator A5081457776 @default.
- W4386475757 date "2023-09-06" @default.
- W4386475757 modified "2023-10-17" @default.
- W4386475757 title "Slow Freezing of Preserved Boar Sperm: Comparison of Conventional and Automated Techniques on Post-Thaw Functional Quality by a New Combination of Sperm Function Tests" @default.
- W4386475757 cites W1965623516 @default.
- W4386475757 cites W1974921952 @default.
- W4386475757 cites W1976654942 @default.
- W4386475757 cites W1998623059 @default.
- W4386475757 cites W2001748786 @default.
- W4386475757 cites W2044165261 @default.
- W4386475757 cites W2086107609 @default.
- W4386475757 cites W2086474578 @default.
- W4386475757 cites W2089613525 @default.
- W4386475757 cites W2107305583 @default.
- W4386475757 cites W2115395629 @default.
- W4386475757 cites W2426290033 @default.
- W4386475757 cites W2524661281 @default.
- W4386475757 cites W2551260326 @default.
- W4386475757 cites W2625677330 @default.
- W4386475757 cites W2759549951 @default.
- W4386475757 cites W2770242134 @default.
- W4386475757 cites W2791760122 @default.
- W4386475757 cites W2800313233 @default.
- W4386475757 cites W2800396931 @default.
- W4386475757 cites W2803959276 @default.
- W4386475757 cites W2898194234 @default.
- W4386475757 cites W2904689147 @default.
- W4386475757 cites W2905555628 @default.
- W4386475757 cites W2969327403 @default.
- W4386475757 cites W3011361605 @default.
- W4386475757 cites W3049150285 @default.
- W4386475757 cites W3049343914 @default.
- W4386475757 cites W3087835116 @default.
- W4386475757 cites W3097048647 @default.
- W4386475757 cites W3123479304 @default.
- W4386475757 cites W3127661175 @default.
- W4386475757 cites W3129308406 @default.
- W4386475757 cites W3138026758 @default.
- W4386475757 cites W3173903634 @default.
- W4386475757 cites W3174914199 @default.
- W4386475757 cites W3194895873 @default.
- W4386475757 cites W3212429192 @default.
- W4386475757 cites W4280511559 @default.
- W4386475757 cites W4297464207 @default.
- W4386475757 doi "https://doi.org/10.3390/ani13182826" @default.
- W4386475757 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/37760225" @default.
- W4386475757 hasPublicationYear "2023" @default.
- W4386475757 type Work @default.
- W4386475757 citedByCount "0" @default.
- W4386475757 crossrefType "journal-article" @default.
- W4386475757 hasAuthorship W4386475757A5020033680 @default.
- W4386475757 hasAuthorship W4386475757A5026474041 @default.
- W4386475757 hasAuthorship W4386475757A5040024728 @default.
- W4386475757 hasAuthorship W4386475757A5052129391 @default.
- W4386475757 hasAuthorship W4386475757A5081457776 @default.
- W4386475757 hasBestOaLocation W43864757571 @default.
- W4386475757 hasConcept C12554922 @default.
- W4386475757 hasConcept C16685009 @default.
- W4386475757 hasConcept C179933525 @default.
- W4386475757 hasConcept C185592680 @default.
- W4386475757 hasConcept C196843134 @default.
- W4386475757 hasConcept C2776151105 @default.
- W4386475757 hasConcept C2777359717 @default.
- W4386475757 hasConcept C2777632594 @default.
- W4386475757 hasConcept C2778252442 @default.
- W4386475757 hasConcept C2780301668 @default.
- W4386475757 hasConcept C2780829032 @default.
- W4386475757 hasConcept C2781087480 @default.
- W4386475757 hasConcept C55493867 @default.
- W4386475757 hasConcept C58207958 @default.
- W4386475757 hasConcept C71924100 @default.
- W4386475757 hasConcept C86803240 @default.
- W4386475757 hasConcept C95444343 @default.
- W4386475757 hasConceptScore W4386475757C12554922 @default.
- W4386475757 hasConceptScore W4386475757C16685009 @default.
- W4386475757 hasConceptScore W4386475757C179933525 @default.
- W4386475757 hasConceptScore W4386475757C185592680 @default.
- W4386475757 hasConceptScore W4386475757C196843134 @default.
- W4386475757 hasConceptScore W4386475757C2776151105 @default.
- W4386475757 hasConceptScore W4386475757C2777359717 @default.
- W4386475757 hasConceptScore W4386475757C2777632594 @default.
- W4386475757 hasConceptScore W4386475757C2778252442 @default.
- W4386475757 hasConceptScore W4386475757C2780301668 @default.
- W4386475757 hasConceptScore W4386475757C2780829032 @default.
- W4386475757 hasConceptScore W4386475757C2781087480 @default.
- W4386475757 hasConceptScore W4386475757C55493867 @default.
- W4386475757 hasConceptScore W4386475757C58207958 @default.
- W4386475757 hasConceptScore W4386475757C71924100 @default.
- W4386475757 hasConceptScore W4386475757C86803240 @default.
- W4386475757 hasConceptScore W4386475757C95444343 @default.
- W4386475757 hasIssue "18" @default.
- W4386475757 hasLocation W43864757571 @default.