FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4386476500> ?p ?o ?g. }

• W4386476500 abstract "Abstract Background Lack of insight into factors that determine purity and quality of human iPSC (hiPSC)-derived neo-cartilage precludes applications of this powerful technology towards regenerative solutions in the clinical setting. Here we set out to generate methylome-wide landscapes of hiPSC derived neo-cartilages from different tissues-of-origin and integrated transcriptome-wide data to identify aberrant set-points of transcription. Methods We applied in vitro chondrogenesis using hiPSCs generated from two different tissue sources; skin fibroblasts and articular cartilage. Upon differentiation towards chondrocytes these are referred to as hFiCs and hCiC, respectively. Genome-wide DNA methylation and RNA sequencing datasets were generated of the hiPSC-derived neo-cartilages, and the epigenetically-regulated transcriptome was compared to that of neo-cartilage deposited by human primary articular cartilage (hPAC). Results Methylome-wide landscapes of neo-cartilages of hiPSCs reprogrammed from two different somatic tissues were 85% similar to that of hPACs. By integration of transcriptome-wide data, changes in transcriptionally active CpGs between hCiC relative to hPAC were prioritized. Among CpG-gene pairs lower expressed in hCiCs relative to hPACs, we identified genes such as MGP, GDF5 , and CHAD enriched in closely related pathways and involved in cartilage development that likely mark phenotypic differences in chondrocyte states. Vice versa, among CpG-gene pairs higher expressed, we identified genes such as KIF1A or NKX2-2 enriched in neurogenic pathways and likely reflected off target differentiation. Conclusions We did not find significant variation between the different tissue sources of the hiPSCs, suggesting that application of a robust differentiation protocol such as we applied here is more important as compared to the epigenetic memory of the cells of origin. Results of our study could be further exploited to improve quality, purity, and maturity of hiPSC derived neo-cartilage matrix, ultimately to realize introduction of sustainable, hiPSC derived neo-cartilage implantation into clinical practice." @default.
• W4386476500 created "2023-09-07" @default.
• W4386476500 creator A5008911804 @default.
• W4386476500 creator A5024298941 @default.
• W4386476500 creator A5032505274 @default.
• W4386476500 creator A5034436231 @default.
• W4386476500 creator A5041292097 @default.
• W4386476500 creator A5048989280 @default.
• W4386476500 creator A5054910477 @default.
• W4386476500 creator A5072924762 @default.
• W4386476500 creator A5078308119 @default.
• W4386476500 creator A5082352019 @default.
• W4386476500 creator A5085927001 @default.
• W4386476500 creator A5088267152 @default.
• W4386476500 date "2023-09-06" @default.
• W4386476500 modified "2023-09-27" @default.
• W4386476500 title "The role of DNA methylation in chondrogenesis of human iPSCs as a stable marker of cartilage quality" @default.
• W4386476500 cites W1975528145 @default.
• W4386476500 cites W2019229253 @default.
• W4386476500 cites W2029485666 @default.
• W4386476500 cites W2039533211 @default.
• W4386476500 cites W2051272245 @default.
• W4386476500 cites W2052878953 @default.
• W4386476500 cites W2065751798 @default.
• W4386476500 cites W2075899152 @default.
• W4386476500 cites W2082223061 @default.
• W4386476500 cites W2092759174 @default.
• W4386476500 cites W2101471525 @default.
• W4386476500 cites W2101870168 @default.
• W4386476500 cites W2102560467 @default.
• W4386476500 cites W2126463435 @default.
• W4386476500 cites W2134526812 @default.
• W4386476500 cites W2156726032 @default.
• W4386476500 cites W2166628699 @default.
• W4386476500 cites W2168447003 @default.
• W4386476500 cites W2169456326 @default.
• W4386476500 cites W2179438025 @default.
• W4386476500 cites W2298870809 @default.
• W4386476500 cites W2485841399 @default.
• W4386476500 cites W2567721002 @default.
• W4386476500 cites W2613179652 @default.
• W4386476500 cites W2899333920 @default.
• W4386476500 cites W2908918388 @default.
• W4386476500 cites W3092457640 @default.
• W4386476500 cites W3104533492 @default.
• W4386476500 cites W3108001297 @default.
• W4386476500 cites W3119231555 @default.
• W4386476500 cites W3154962230 @default.
• W4386476500 cites W3161100085 @default.
• W4386476500 cites W3161619490 @default.
• W4386476500 cites W3164386912 @default.
• W4386476500 cites W3182228908 @default.
• W4386476500 cites W3193910581 @default.
• W4386476500 cites W3200082783 @default.
• W4386476500 cites W3209372919 @default.
• W4386476500 cites W4250595858 @default.
• W4386476500 doi "https://doi.org/10.21203/rs.3.rs-3272276/v1" @default.
• W4386476500 hasPublicationYear "2023" @default.
• W4386476500 type Work @default.
• W4386476500 citedByCount "0" @default.
• W4386476500 crossrefType "posted-content" @default.
• W4386476500 hasAuthorship W4386476500A5008911804 @default.
• W4386476500 hasAuthorship W4386476500A5024298941 @default.
• W4386476500 hasAuthorship W4386476500A5032505274 @default.
• W4386476500 hasAuthorship W4386476500A5034436231 @default.
• W4386476500 hasAuthorship W4386476500A5041292097 @default.
• W4386476500 hasAuthorship W4386476500A5048989280 @default.
• W4386476500 hasAuthorship W4386476500A5054910477 @default.
• W4386476500 hasAuthorship W4386476500A5072924762 @default.
• W4386476500 hasAuthorship W4386476500A5078308119 @default.
• W4386476500 hasAuthorship W4386476500A5082352019 @default.
• W4386476500 hasAuthorship W4386476500A5085927001 @default.
• W4386476500 hasAuthorship W4386476500A5088267152 @default.
• W4386476500 hasBestOaLocation W43864765001 @default.
• W4386476500 hasConcept C104317684 @default.
• W4386476500 hasConcept C105702510 @default.
• W4386476500 hasConcept C134305767 @default.
• W4386476500 hasConcept C140173407 @default.
• W4386476500 hasConcept C150194340 @default.
• W4386476500 hasConcept C162317418 @default.
• W4386476500 hasConcept C190727270 @default.
• W4386476500 hasConcept C2780550940 @default.
• W4386476500 hasConcept C2781403057 @default.
• W4386476500 hasConcept C33288867 @default.
• W4386476500 hasConcept C35496256 @default.
• W4386476500 hasConcept C54355233 @default.
• W4386476500 hasConcept C70721500 @default.
• W4386476500 hasConcept C86803240 @default.
• W4386476500 hasConcept C95444343 @default.
• W4386476500 hasConceptScore W4386476500C104317684 @default.
• W4386476500 hasConceptScore W4386476500C105702510 @default.
• W4386476500 hasConceptScore W4386476500C134305767 @default.
• W4386476500 hasConceptScore W4386476500C140173407 @default.
• W4386476500 hasConceptScore W4386476500C150194340 @default.
• W4386476500 hasConceptScore W4386476500C162317418 @default.
• W4386476500 hasConceptScore W4386476500C190727270 @default.
• W4386476500 hasConceptScore W4386476500C2780550940 @default.
• W4386476500 hasConceptScore W4386476500C2781403057 @default.
• W4386476500 hasConceptScore W4386476500C33288867 @default.
• W4386476500 hasConceptScore W4386476500C35496256 @default.

• next