Matches in SemOpenAlex for { <https://semopenalex.org/work/W4386502013> ?p ?o ?g. }
- W4386502013 abstract "Phytochromes are photoreceptor proteins with a bilin chromophore that undergo photoconversion between two spectrally different forms, Pr and Pfr. Three domains, termed PAS, GAF, and PHY domains, constitute the N-terminal photosensory chromophore module (PCM); the C-terminus is often a histidine kinase module. In the Agrobacterium fabrum phytochrome Agp1, the autophosphorylation activity of the histidine kinase is high in the Pr and low in the Pfr form. Crystal structure analyses of PCMs suggest flexibility around position 308 in the Pr but not in the Pfr form. Here, we performed time-resolved fluorescence anisotropy measurements with different Agp1 mutants, each with a single cysteine residue at various positions. The fluorophore label Atto-488 was attached to each mutant, and time-resolved fluorescence anisotropy was measured in the Pr and Pfr forms. Fluorescence anisotropy curves were fitted with biexponential functions. Differences in the amplitude A2 of the second component between the PCM and the full-length variant indicate a mechanical coupling between position 362 and the histidine kinase. Pr-to-Pfr photoconversion induced no significant changes in the time constant t2 at any position. An intermediate t2 value at position 295, which is located in a compact environment, suggests flexibility around the nearby position 308 in Pr and in Pfr." @default.
- W4386502013 created "2023-09-08" @default.
- W4386502013 creator A5026961297 @default.
- W4386502013 creator A5060193239 @default.
- W4386502013 creator A5071465671 @default.
- W4386502013 creator A5075787427 @default.
- W4386502013 creator A5079879196 @default.
- W4386502013 creator A5089260050 @default.
- W4386502013 creator A5092767876 @default.
- W4386502013 date "2023-09-07" @default.
- W4386502013 modified "2023-10-16" @default.
- W4386502013 title "Time‐resolved fluorescence anisotropy with Atto 488‐labeled phytochrome Agp1 from <i>Agrobacterium fabrum</i>" @default.
- W4386502013 cites W1601437562 @default.
- W4386502013 cites W2009384313 @default.
- W4386502013 cites W2009466802 @default.
- W4386502013 cites W2021609583 @default.
- W4386502013 cites W2025733802 @default.
- W4386502013 cites W2029270622 @default.
- W4386502013 cites W2040490477 @default.
- W4386502013 cites W2050903472 @default.
- W4386502013 cites W2051956860 @default.
- W4386502013 cites W2055831992 @default.
- W4386502013 cites W2062614698 @default.
- W4386502013 cites W2068577967 @default.
- W4386502013 cites W2084827644 @default.
- W4386502013 cites W2125018901 @default.
- W4386502013 cites W2129995310 @default.
- W4386502013 cites W2133712310 @default.
- W4386502013 cites W2154093011 @default.
- W4386502013 cites W2500412781 @default.
- W4386502013 cites W2505630186 @default.
- W4386502013 cites W2595522384 @default.
- W4386502013 cites W2613294232 @default.
- W4386502013 cites W2901424771 @default.
- W4386502013 cites W3157964783 @default.
- W4386502013 cites W59211883 @default.
- W4386502013 cites W606051889 @default.
- W4386502013 doi "https://doi.org/10.1111/php.13851" @default.
- W4386502013 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/37675785" @default.
- W4386502013 hasPublicationYear "2023" @default.
- W4386502013 type Work @default.
- W4386502013 citedByCount "0" @default.
- W4386502013 crossrefType "journal-article" @default.
- W4386502013 hasAuthorship W4386502013A5026961297 @default.
- W4386502013 hasAuthorship W4386502013A5060193239 @default.
- W4386502013 hasAuthorship W4386502013A5071465671 @default.
- W4386502013 hasAuthorship W4386502013A5075787427 @default.
- W4386502013 hasAuthorship W4386502013A5079879196 @default.
- W4386502013 hasAuthorship W4386502013A5089260050 @default.
- W4386502013 hasAuthorship W4386502013A5092767876 @default.
- W4386502013 hasBestOaLocation W43865020131 @default.
- W4386502013 hasConcept C111357860 @default.
- W4386502013 hasConcept C120665830 @default.
- W4386502013 hasConcept C121332964 @default.
- W4386502013 hasConcept C12554922 @default.
- W4386502013 hasConcept C150109051 @default.
- W4386502013 hasConcept C184235292 @default.
- W4386502013 hasConcept C185592680 @default.
- W4386502013 hasConcept C192468462 @default.
- W4386502013 hasConcept C2778460671 @default.
- W4386502013 hasConcept C2778617687 @default.
- W4386502013 hasConcept C2778905257 @default.
- W4386502013 hasConcept C2986477067 @default.
- W4386502013 hasConcept C515207424 @default.
- W4386502013 hasConcept C55493867 @default.
- W4386502013 hasConcept C59822182 @default.
- W4386502013 hasConcept C75473681 @default.
- W4386502013 hasConcept C86803240 @default.
- W4386502013 hasConcept C90180588 @default.
- W4386502013 hasConcept C91881484 @default.
- W4386502013 hasConcept C97029542 @default.
- W4386502013 hasConceptScore W4386502013C111357860 @default.
- W4386502013 hasConceptScore W4386502013C120665830 @default.
- W4386502013 hasConceptScore W4386502013C121332964 @default.
- W4386502013 hasConceptScore W4386502013C12554922 @default.
- W4386502013 hasConceptScore W4386502013C150109051 @default.
- W4386502013 hasConceptScore W4386502013C184235292 @default.
- W4386502013 hasConceptScore W4386502013C185592680 @default.
- W4386502013 hasConceptScore W4386502013C192468462 @default.
- W4386502013 hasConceptScore W4386502013C2778460671 @default.
- W4386502013 hasConceptScore W4386502013C2778617687 @default.
- W4386502013 hasConceptScore W4386502013C2778905257 @default.
- W4386502013 hasConceptScore W4386502013C2986477067 @default.
- W4386502013 hasConceptScore W4386502013C515207424 @default.
- W4386502013 hasConceptScore W4386502013C55493867 @default.
- W4386502013 hasConceptScore W4386502013C59822182 @default.
- W4386502013 hasConceptScore W4386502013C75473681 @default.
- W4386502013 hasConceptScore W4386502013C86803240 @default.
- W4386502013 hasConceptScore W4386502013C90180588 @default.
- W4386502013 hasConceptScore W4386502013C91881484 @default.
- W4386502013 hasConceptScore W4386502013C97029542 @default.
- W4386502013 hasLocation W43865020131 @default.
- W4386502013 hasLocation W43865020132 @default.
- W4386502013 hasOpenAccess W4386502013 @default.
- W4386502013 hasPrimaryLocation W43865020131 @default.
- W4386502013 hasRelatedWork W2044875267 @default.
- W4386502013 hasRelatedWork W2060782735 @default.
- W4386502013 hasRelatedWork W2066627565 @default.
- W4386502013 hasRelatedWork W2143621248 @default.
- W4386502013 hasRelatedWork W2282410411 @default.