Matches in SemOpenAlex for { <https://semopenalex.org/work/W4386508049> ?p ?o ?g. }
- W4386508049 abstract "Opioid overdose is the leading cause of drug overdose lethality, posing an urgent need for investigation. The key brain region for inspiratory rhythm regulation and opioid-induced respiratory depression (OIRD) is the preBötzinger Complex (preBötC) and current knowledge has mainly been obtained from animal systems. This study aims to establish a protocol to generate human preBötC neurons from induced pluripotent cells (iPSCs) and develop an opioid overdose and recovery model utilizing these iPSC-preBötC neurons. A de novo protocol to differentiate preBötC-like neurons from human iPSCs is established. These neurons express essential preBötC markers analyzed by immunocytochemistry and demonstrate expected electrophysiological responses to preBötC modulators analyzed by patch clamp electrophysiology. The correlation of the specific biomarkers and function analysis strongly suggests a preBötC-like phenotype. Moreover, the dose-dependent inhibition of these neurons' activity is demonstrated for four different opioids with identified IC50's comparable to the literature. Inhibition is rescued by naloxone in a concentration-dependent manner. This iPSC-preBötC mimic is crucial for investigating OIRD and combating the overdose crisis and a first step for the integration of a functional overdose model into microphysiological systems." @default.
- W4386508049 created "2023-09-08" @default.
- W4386508049 creator A5010320681 @default.
- W4386508049 creator A5020650188 @default.
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- W4386508049 creator A5026618894 @default.
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- W4386508049 creator A5084386025 @default.
- W4386508049 creator A5086517204 @default.
- W4386508049 date "2023-09-07" @default.
- W4386508049 modified "2023-10-14" @default.
- W4386508049 title "Human IPSC‐Derived PreBötC‐Like Neurons and Development of an Opiate Overdose and Recovery Model" @default.
- W4386508049 cites W1201297550 @default.
- W4386508049 cites W1529085124 @default.
- W4386508049 cites W1544391143 @default.
- W4386508049 cites W1560498709 @default.
- W4386508049 cites W1571900525 @default.
- W4386508049 cites W169575167 @default.
- W4386508049 cites W1845987094 @default.
- W4386508049 cites W1879018584 @default.
- W4386508049 cites W1931371976 @default.
- W4386508049 cites W1964026025 @default.
- W4386508049 cites W1968086599 @default.
- W4386508049 cites W1977105806 @default.
- W4386508049 cites W1977735902 @default.
- W4386508049 cites W1979031324 @default.
- W4386508049 cites W1979861391 @default.
- W4386508049 cites W1986015216 @default.
- W4386508049 cites W1993893814 @default.
- W4386508049 cites W2000744528 @default.
- W4386508049 cites W2003737145 @default.
- W4386508049 cites W2010934845 @default.
- W4386508049 cites W2015046028 @default.
- W4386508049 cites W2018053416 @default.
- W4386508049 cites W2024238924 @default.
- W4386508049 cites W2024929900 @default.
- W4386508049 cites W2026289833 @default.
- W4386508049 cites W2028043512 @default.
- W4386508049 cites W2034846738 @default.
- W4386508049 cites W2037233574 @default.
- W4386508049 cites W2037688283 @default.
- W4386508049 cites W2044029619 @default.
- W4386508049 cites W2048249977 @default.
- W4386508049 cites W2049944651 @default.
- W4386508049 cites W2052881256 @default.
- W4386508049 cites W2058965831 @default.
- W4386508049 cites W2063604509 @default.
- W4386508049 cites W2079140754 @default.
- W4386508049 cites W2079708017 @default.
- W4386508049 cites W2079883339 @default.
- W4386508049 cites W2080362492 @default.
- W4386508049 cites W2082852301 @default.
- W4386508049 cites W2085069683 @default.
- W4386508049 cites W2085883872 @default.
- W4386508049 cites W2089264352 @default.
- W4386508049 cites W2094353622 @default.
- W4386508049 cites W2102678193 @default.
- W4386508049 cites W2104899979 @default.
- W4386508049 cites W2108163830 @default.
- W4386508049 cites W2108815207 @default.
- W4386508049 cites W2115540466 @default.
- W4386508049 cites W2116099756 @default.
- W4386508049 cites W2116633404 @default.
- W4386508049 cites W2119693721 @default.
- W4386508049 cites W2120070895 @default.
- W4386508049 cites W2122986030 @default.
- W4386508049 cites W2132468342 @default.
- W4386508049 cites W2139453672 @default.
- W4386508049 cites W2146631034 @default.
- W4386508049 cites W2154628135 @default.
- W4386508049 cites W2159395955 @default.
- W4386508049 cites W2160966356 @default.
- W4386508049 cites W2166226260 @default.
- W4386508049 cites W2168996269 @default.
- W4386508049 cites W2169701035 @default.
- W4386508049 cites W2171627702 @default.
- W4386508049 cites W2309270306 @default.
- W4386508049 cites W2339389944 @default.
- W4386508049 cites W2340876918 @default.
- W4386508049 cites W2394730474 @default.
- W4386508049 cites W2412243856 @default.
- W4386508049 cites W2476031803 @default.
- W4386508049 cites W2480074253 @default.
- W4386508049 cites W2505292440 @default.
- W4386508049 cites W2512213814 @default.
- W4386508049 cites W2520164020 @default.
- W4386508049 cites W2567433872 @default.
- W4386508049 cites W2579679755 @default.
- W4386508049 cites W2588024302 @default.
- W4386508049 cites W2597578733 @default.
- W4386508049 cites W2605353601 @default.
- W4386508049 cites W2760847338 @default.
- W4386508049 cites W2769348959 @default.
- W4386508049 cites W2791507458 @default.
- W4386508049 cites W2792962347 @default.
- W4386508049 cites W2797188567 @default.
- W4386508049 cites W2800072530 @default.
- W4386508049 cites W2905380016 @default.