FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4386523831> ?p ?o ?g. }

• W4386523831 endingPage "13787" @default.
• W4386523831 startingPage "13779" @default.
• W4386523831 abstract "Target proteins are often stabilized after binding with a ligand and thereby typically become more resistant to denaturation. Based on this phenomenon, several methods without the need to covalently modify the ligand have been developed to identify target proteins for a specific ligand. These methods usually employ complicated workflows with high cost and limited throughput. Here, we develop an iso-pH shift assay (ipHSA) method, a proteome-wide target identification method that detects ligand-induced protein solubility shifts by precipitating proteins with a single concentration of acidic agent followed by protein quantification via data-independent acquisition (DIA). Using a pan-kinase inhibitor, staurosporine, we demonstrated that ipHSA increased throughput compared to the previously developed pH-dependent protein precipitation (pHDPP) method. ipHSA was found to have high complementarity in staurosporine target identification compared with the improved isothermal shift assay (iTSA) and isosolvent shift assay (iSSA) using DIA instead of tandem mass tags (TMTs) for quantification. To further improve target identification sensitivity, we developed an integrated protein solubility shift assay (IPSSA) by pooling the supernatants yielded from ipHSA, iTSA, and iSSA methods. IPSSA exhibited increased sensitivity in screening staurosporine targets by 38, 29, and 38% compared to individual methods. Increasing the number of replicate experiments further enhanced the sensitivity of target identification. Meanwhile, IPSSA also improved the throughput and reduced the cost compared with previous methods. As a fast and efficient tool for drug target identification, IPSSA is expected to have broad applications in the study of the mechanism of action." @default.
• W4386523831 created "2023-09-08" @default.
• W4386523831 creator A5003642180 @default.
• W4386523831 creator A5004349988 @default.
• W4386523831 creator A5008218058 @default.
• W4386523831 creator A5022401276 @default.
• W4386523831 creator A5058328532 @default.
• W4386523831 creator A5064367006 @default.
• W4386523831 creator A5066126919 @default.
• W4386523831 date "2023-09-07" @default.
• W4386523831 modified "2023-10-17" @default.
• W4386523831 title "Integrated Protein Solubility Shift Assays for Comprehensive Drug Target Identification on a Proteome-Wide Scale" @default.
• W4386523831 cites W2018126724 @default.
• W4386523831 cites W2026542427 @default.
• W4386523831 cites W2067400543 @default.
• W4386523831 cites W2100772783 @default.
• W4386523831 cites W2110151487 @default.
• W4386523831 cites W2131321938 @default.
• W4386523831 cites W2173488267 @default.
• W4386523831 cites W2524972396 @default.
• W4386523831 cites W2769377508 @default.
• W4386523831 cites W2782205417 @default.
• W4386523831 cites W2787587553 @default.
• W4386523831 cites W2903493617 @default.
• W4386523831 cites W2952847594 @default.
• W4386523831 cites W2954110881 @default.
• W4386523831 cites W2976915124 @default.
• W4386523831 cites W2984906251 @default.
• W4386523831 cites W2993960317 @default.
• W4386523831 cites W3000219741 @default.
• W4386523831 cites W3006371860 @default.
• W4386523831 cites W3010095407 @default.
• W4386523831 cites W3014288429 @default.
• W4386523831 cites W3080123711 @default.
• W4386523831 cites W3115609147 @default.
• W4386523831 cites W3119767006 @default.
• W4386523831 cites W3131784305 @default.
• W4386523831 cites W3158298637 @default.
• W4386523831 cites W4206637925 @default.
• W4386523831 cites W4220695070 @default.
• W4386523831 cites W4225088714 @default.
• W4386523831 cites W4225930576 @default.
• W4386523831 cites W4229025312 @default.
• W4386523831 cites W4229053149 @default.
• W4386523831 cites W4309242869 @default.
• W4386523831 doi "https://doi.org/10.1021/acs.analchem.3c00072" @default.
• W4386523831 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/37676971" @default.
• W4386523831 hasPublicationYear "2023" @default.
• W4386523831 type Work @default.
• W4386523831 citedByCount "0" @default.
• W4386523831 crossrefType "journal-article" @default.
• W4386523831 hasAuthorship W4386523831A5003642180 @default.
• W4386523831 hasAuthorship W4386523831A5004349988 @default.
• W4386523831 hasAuthorship W4386523831A5008218058 @default.
• W4386523831 hasAuthorship W4386523831A5022401276 @default.
• W4386523831 hasAuthorship W4386523831A5058328532 @default.
• W4386523831 hasAuthorship W4386523831A5064367006 @default.
• W4386523831 hasAuthorship W4386523831A5066126919 @default.
• W4386523831 hasConcept C104317684 @default.
• W4386523831 hasConcept C104397665 @default.
• W4386523831 hasConcept C109095088 @default.
• W4386523831 hasConcept C116569031 @default.
• W4386523831 hasConcept C155574463 @default.
• W4386523831 hasConcept C170493617 @default.
• W4386523831 hasConcept C178790620 @default.
• W4386523831 hasConcept C184235292 @default.
• W4386523831 hasConcept C185592680 @default.
• W4386523831 hasConcept C2779084600 @default.
• W4386523831 hasConcept C2780430158 @default.
• W4386523831 hasConcept C43617362 @default.
• W4386523831 hasConcept C51323132 @default.
• W4386523831 hasConcept C55493867 @default.
• W4386523831 hasConcept C70721500 @default.
• W4386523831 hasConcept C86803240 @default.
• W4386523831 hasConcept C97029542 @default.
• W4386523831 hasConceptScore W4386523831C104317684 @default.
• W4386523831 hasConceptScore W4386523831C104397665 @default.
• W4386523831 hasConceptScore W4386523831C109095088 @default.
• W4386523831 hasConceptScore W4386523831C116569031 @default.
• W4386523831 hasConceptScore W4386523831C155574463 @default.
• W4386523831 hasConceptScore W4386523831C170493617 @default.
• W4386523831 hasConceptScore W4386523831C178790620 @default.
• W4386523831 hasConceptScore W4386523831C184235292 @default.
• W4386523831 hasConceptScore W4386523831C185592680 @default.
• W4386523831 hasConceptScore W4386523831C2779084600 @default.
• W4386523831 hasConceptScore W4386523831C2780430158 @default.
• W4386523831 hasConceptScore W4386523831C43617362 @default.
• W4386523831 hasConceptScore W4386523831C51323132 @default.
• W4386523831 hasConceptScore W4386523831C55493867 @default.
• W4386523831 hasConceptScore W4386523831C70721500 @default.
• W4386523831 hasConceptScore W4386523831C86803240 @default.
• W4386523831 hasConceptScore W4386523831C97029542 @default.
• W4386523831 hasFunder F4320321001 @default.
• W4386523831 hasFunder F4320321540 @default.
• W4386523831 hasFunder F4320334825 @default.
• W4386523831 hasIssue "37" @default.
• W4386523831 hasLocation W43865238311 @default.
• W4386523831 hasLocation W43865238312 @default.

• next