Matches in SemOpenAlex for { <https://semopenalex.org/work/W4386526210> ?p ?o ?g. }
- W4386526210 abstract "We have previously demonstrated that a pathologic downregulation of peroxisome proliferator-activated receptor-gamma coactivator 1-alpha (PGC1α) within the intestinal epithelium contributes to the pathogenesis of inflammatory bowel disease (IBD). However, the mechanism underlying downregulation of PGC1α expression and activity during IBD is not yet clear.Mice (male; C57Bl/6, Villincre/+;Pgc1afl/fl mice, and Pgc1afl/fl) were subjected to experimental colitis and treated with nicotinamide riboside. Western blot, high-resolution respirometry, nicotinamide adenine dinucleotide (NAD+) quantification, and immunoprecipitation were used to in this study.We demonstrate a significant depletion in the NAD+ levels within the intestinal epithelium of mice undergoing experimental colitis, as well as humans with ulcerative colitis. While we found no decrease in the levels of NAD+-synthesizing enzymes within the intestinal epithelium of mice undergoing experimental colitis, we did find an increase in the mRNA level, as well as the enzymatic activity, of the NAD+-consuming enzyme poly(ADP-ribose) polymerase-1 (PARP1). Treatment of mice undergoing experimental colitis with an NAD+ precursor reduced the severity of colitis, restored mitochondrial function, and increased active PGC1α levels; however, NAD+ repletion did not benefit transgenic mice that lack PGC1α within the intestinal epithelium, suggesting that the therapeutic effects require an intact PGC1α axis.Our results emphasize the importance of PGC1α expression to both mitochondrial health and homeostasis within the intestinal epithelium and suggest a novel therapeutic approach for disease management. These findings also provide a mechanistic basis for clinical trials of nicotinamide riboside in IBD patients." @default.
- W4386526210 created "2023-09-08" @default.
- W4386526210 creator A5013913026 @default.
- W4386526210 creator A5016911758 @default.
- W4386526210 creator A5020724101 @default.
- W4386526210 creator A5022467380 @default.
- W4386526210 creator A5040944004 @default.
- W4386526210 creator A5046225712 @default.
- W4386526210 creator A5060852282 @default.
- W4386526210 creator A5064167863 @default.
- W4386526210 creator A5067550204 @default.
- W4386526210 creator A5067754282 @default.
- W4386526210 creator A5070170389 @default.
- W4386526210 creator A5070875900 @default.
- W4386526210 creator A5079768867 @default.
- W4386526210 date "2023-09-07" @default.
- W4386526210 modified "2023-09-27" @default.
- W4386526210 title "Epithelial NAD+ depletion drives mitochondrial dysfunction and contributes to intestinal inflammation" @default.
- W4386526210 cites W1621684029 @default.
- W4386526210 cites W1930879233 @default.
- W4386526210 cites W1932093217 @default.
- W4386526210 cites W1963711485 @default.
- W4386526210 cites W1979504051 @default.
- W4386526210 cites W1989299025 @default.
- W4386526210 cites W1992591741 @default.
- W4386526210 cites W1996845948 @default.
- W4386526210 cites W1997312454 @default.
- W4386526210 cites W2006876455 @default.
- W4386526210 cites W2012401588 @default.
- W4386526210 cites W2016811610 @default.
- W4386526210 cites W2018075167 @default.
- W4386526210 cites W2018707752 @default.
- W4386526210 cites W2020304127 @default.
- W4386526210 cites W2021073550 @default.
- W4386526210 cites W2026029673 @default.
- W4386526210 cites W2027904284 @default.
- W4386526210 cites W2029667275 @default.
- W4386526210 cites W2036552077 @default.
- W4386526210 cites W2037746176 @default.
- W4386526210 cites W2049547722 @default.
- W4386526210 cites W2076252987 @default.
- W4386526210 cites W2076633494 @default.
- W4386526210 cites W2094778049 @default.
- W4386526210 cites W2097175319 @default.
- W4386526210 cites W2097818551 @default.
- W4386526210 cites W2102186047 @default.
- W4386526210 cites W2105404724 @default.
- W4386526210 cites W2113183122 @default.
- W4386526210 cites W2123016403 @default.
- W4386526210 cites W2123341391 @default.
- W4386526210 cites W2126103084 @default.
- W4386526210 cites W2126337135 @default.
- W4386526210 cites W2133444149 @default.
- W4386526210 cites W2141688163 @default.
- W4386526210 cites W2146830757 @default.
- W4386526210 cites W2148391873 @default.
- W4386526210 cites W2152101492 @default.
- W4386526210 cites W2155208388 @default.
- W4386526210 cites W2165398964 @default.
- W4386526210 cites W2168333087 @default.
- W4386526210 cites W2205750127 @default.
- W4386526210 cites W2230823892 @default.
- W4386526210 cites W2273601982 @default.
- W4386526210 cites W2294582511 @default.
- W4386526210 cites W2312701756 @default.
- W4386526210 cites W2343193821 @default.
- W4386526210 cites W2394799259 @default.
- W4386526210 cites W2414222516 @default.
- W4386526210 cites W2470982690 @default.
- W4386526210 cites W2537034621 @default.
- W4386526210 cites W2539630895 @default.
- W4386526210 cites W2593695507 @default.
- W4386526210 cites W2618676341 @default.
- W4386526210 cites W2729660848 @default.
- W4386526210 cites W2753842728 @default.
- W4386526210 cites W2761535229 @default.
- W4386526210 cites W2795692601 @default.
- W4386526210 cites W2811006156 @default.
- W4386526210 cites W2883385563 @default.
- W4386526210 cites W2884267073 @default.
- W4386526210 cites W2887656633 @default.
- W4386526210 cites W2888475558 @default.
- W4386526210 cites W2893860636 @default.
- W4386526210 cites W2907177946 @default.
- W4386526210 cites W2908458267 @default.
- W4386526210 cites W2932874347 @default.
- W4386526210 cites W2938255449 @default.
- W4386526210 cites W2940938526 @default.
- W4386526210 cites W2951609462 @default.
- W4386526210 cites W2953786622 @default.
- W4386526210 cites W2963684339 @default.
- W4386526210 cites W2974493130 @default.
- W4386526210 cites W2990279159 @default.
- W4386526210 cites W2992748915 @default.
- W4386526210 cites W3005675840 @default.
- W4386526210 cites W3006150521 @default.
- W4386526210 cites W3009711482 @default.
- W4386526210 cites W3010636379 @default.
- W4386526210 cites W3011117765 @default.
- W4386526210 cites W3016864895 @default.