FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4386558574> ?p ?o ?g. }

• W4386558574 endingPage "e0285063" @default.
• W4386558574 startingPage "e0285063" @default.
• W4386558574 abstract "Introduction The persistence of tuberculosis (TB) infection in some patients after treatment has highlighted the importance of drug susceptibility testing (DST). This study aimed to determine the drug susceptibility patterns of Mycobacterium tuberculosis ( M . tuberculosis ) isolates from pulmonary TB (PTB) patients in Central and Southern Ethiopia. Methods A health institution-based cross-sectional study was conducted between July 2021 and April 2022. Sputum samples were collected from newly diagnosed smear microscopy and/or Xpert MTB/RIF-positive PTB patients. The samples were processed and cultivated in Lowenstein-Jensen (LJ) pyruvate and glycerol medium. M . tuberculosis isolates were identified using polymerase chain reaction (PCR) based region of difference 9 (RD9) deletion typing. Phenotypic DST patterns of the isolates were characterized using the BACTEC MGIT ™ 960 instrument with SIRE kit. Isoniazid (INH) and Rifampicin (RIF) resistant M . tuberculosis isolates were identified using the GenoType ® MTBDR plus assay. Results Sputum samples were collected from 350 PTB patients, 315 (90%) of which were culture-positive, and phenotypic and genotypic DST were determined for 266 and 261 isolates, respectively. Due to invalid results and missing data, 6% (16/266) of the isolates were excluded, while 94% (250/266) were included in the paired analysis. According to the findings, 14.4% (36/250) of the isolates tested positive for resistance to at least one anti-TB drug. Gene mutations were observed only in the rpoB and katG gene loci, indicating RIF and high-level INH resistance. The GenoType ® MTBDR plus assay has a sensitivity of 42% and a specificity of 100% in detecting INH-resistant M . tuberculosis isolates, with a kappa value of 0.56 (95%CI: 0.36–0.76) compared to the BACTEC MGIT ™ DST. The overall discordance between the two methods was 5.6% (14/250) for INH alone and 0% for RIF resistance and MDR-TB (resistance to both INH and RIF) detection. Conclusion This study reveals a higher prevalence of phenotypic and genotypic discordant INH-resistant M . tuberculosis isolates in the study area. The use of whole-genome sequencing (WGS) is essential for gaining a comprehensive understanding of these discrepancies within INH-resistant M . tuberculosis strains." @default.
• W4386558574 created "2023-09-09" @default.
• W4386558574 creator A5000854892 @default.
• W4386558574 creator A5025177862 @default.
• W4386558574 creator A5050600007 @default.
• W4386558574 creator A5066198287 @default.
• W4386558574 creator A5071427636 @default.
• W4386558574 creator A5074953709 @default.
• W4386558574 creator A5082634108 @default.
• W4386558574 creator A5085570865 @default.
• W4386558574 creator A5085844918 @default.
• W4386558574 creator A5092829598 @default.
• W4386558574 creator A5092829599 @default.
• W4386558574 creator A5092829600 @default.
• W4386558574 creator A5008971976 @default.
• W4386558574 date "2023-09-08" @default.
• W4386558574 modified "2023-09-27" @default.
• W4386558574 title "Phenotypic and genotypic drug susceptibility patterns of Mycobacterium tuberculosis isolates from pulmonary tuberculosis patients in Central and Southern Ethiopia" @default.
• W4386558574 cites W1967584362 @default.
• W4386558574 cites W1996458668 @default.
• W4386558574 cites W2055039460 @default.
• W4386558574 cites W2087971638 @default.
• W4386558574 cites W2107340859 @default.
• W4386558574 cites W2128886025 @default.
• W4386558574 cites W2137128333 @default.
• W4386558574 cites W2139040924 @default.
• W4386558574 cites W2142919174 @default.
• W4386558574 cites W2284576719 @default.
• W4386558574 cites W2342220659 @default.
• W4386558574 cites W2472021049 @default.
• W4386558574 cites W2523607113 @default.
• W4386558574 cites W2573909372 @default.
• W4386558574 cites W2576073187 @default.
• W4386558574 cites W2729935006 @default.
• W4386558574 cites W2747556736 @default.
• W4386558574 cites W2761842854 @default.
• W4386558574 cites W2781002339 @default.
• W4386558574 cites W2800888530 @default.
• W4386558574 cites W2807171064 @default.
• W4386558574 cites W2891180638 @default.
• W4386558574 cites W2935974890 @default.
• W4386558574 cites W2946864708 @default.
• W4386558574 cites W2990945000 @default.
• W4386558574 cites W3041598956 @default.
• W4386558574 cites W3107060353 @default.
• W4386558574 cites W3115757970 @default.
• W4386558574 cites W3116485762 @default.
• W4386558574 cites W3126203365 @default.
• W4386558574 cites W3157680660 @default.
• W4386558574 cites W3163682376 @default.
• W4386558574 cites W3203881199 @default.
• W4386558574 cites W3205037849 @default.
• W4386558574 cites W4206312654 @default.
• W4386558574 cites W4210937345 @default.
• W4386558574 cites W4283023994 @default.
• W4386558574 cites W4294667136 @default.
• W4386558574 cites W4297215774 @default.
• W4386558574 cites W4303709972 @default.
• W4386558574 cites W4306404524 @default.
• W4386558574 doi "https://doi.org/10.1371/journal.pone.0285063" @default.
• W4386558574 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/37682820" @default.
• W4386558574 hasPublicationYear "2023" @default.
• W4386558574 type Work @default.
• W4386558574 citedByCount "0" @default.
• W4386558574 crossrefType "journal-article" @default.
• W4386558574 hasAuthorship W4386558574A5000854892 @default.
• W4386558574 hasAuthorship W4386558574A5008971976 @default.
• W4386558574 hasAuthorship W4386558574A5025177862 @default.
• W4386558574 hasAuthorship W4386558574A5050600007 @default.
• W4386558574 hasAuthorship W4386558574A5066198287 @default.
• W4386558574 hasAuthorship W4386558574A5071427636 @default.
• W4386558574 hasAuthorship W4386558574A5074953709 @default.
• W4386558574 hasAuthorship W4386558574A5082634108 @default.
• W4386558574 hasAuthorship W4386558574A5085570865 @default.
• W4386558574 hasAuthorship W4386558574A5085844918 @default.
• W4386558574 hasAuthorship W4386558574A5092829598 @default.
• W4386558574 hasAuthorship W4386558574A5092829599 @default.
• W4386558574 hasAuthorship W4386558574A5092829600 @default.
• W4386558574 hasBestOaLocation W43865585741 @default.
• W4386558574 hasConcept C104317684 @default.
• W4386558574 hasConcept C114851261 @default.
• W4386558574 hasConcept C135763542 @default.
• W4386558574 hasConcept C142724271 @default.
• W4386558574 hasConcept C159047783 @default.
• W4386558574 hasConcept C2776301714 @default.
• W4386558574 hasConcept C2776967927 @default.
• W4386558574 hasConcept C2777975735 @default.
• W4386558574 hasConcept C2778340795 @default.
• W4386558574 hasConcept C2778607973 @default.
• W4386558574 hasConcept C2781069245 @default.
• W4386558574 hasConcept C2781209916 @default.
• W4386558574 hasConcept C54355233 @default.
• W4386558574 hasConcept C71924100 @default.
• W4386558574 hasConcept C86803240 @default.
• W4386558574 hasConcept C89423630 @default.
• W4386558574 hasConceptScore W4386558574C104317684 @default.
• W4386558574 hasConceptScore W4386558574C114851261 @default.
• W4386558574 hasConceptScore W4386558574C135763542 @default.

• next