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• W4386562873 abstract "In coffee ( Coffea arabica L.), male sterility is a prerequisite for the exploitation of heterosis since it provides an efficient and reliable method for the production of hybrid seeds. Given its relevance, the objective of this study was to identify male-sterile genotypes within the Colombian Coffee Collection that can be used in genetic improvement. For this purpose, Ethiopian germplasm and progenies derived from hybrids between C . arabica x C . canephora were explored between 2017 and 2021. In the first stage, genotypes without visual presence of pollen were preselected in the field, followed by selection through staining and verification of male sterility and female fertility through directed crosses (directed, reciprocal and selfing). In this stage, 9,753 trees were explored, preselecting 2.4% due to visual absence of pollen. The staining of structures allowed us to confirm the lack or sporadic production of pollen in 23 individuals of Ethiopian origin. The results of the directed crosses led to the identification of 11 male-sterile and 12 partially male-sterile genotypes belonging to 15 accessions. In all cases, the individuals were characterized by the presence of anthers but with an absence or low content of pollen, which is why the male sterility is possibly of the sporogenic type. The female receptivity values were between 2.9% and 72.6%, being higher than 30% in five genotypes. These genotypes are a valuable tool for the genetic improvement of C . arabica with the potential to facilitate the use of heterosis and to allow a deeper understanding the development of male gametophytes in the species." @default.
• W4386562873 created "2023-09-10" @default.
• W4386562873 creator A5060312026 @default.
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• W4386562873 date "2023-09-08" @default.
• W4386562873 modified "2023-09-30" @default.
• W4386562873 title "Identification of sources of male sterility in the Colombian Coffee Collection for the genetic improvement of Coffea arabica L." @default.
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• W4386562873 doi "https://doi.org/10.1371/journal.pone.0291264" @default.
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