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- W4386652456 abstract "For quick and quantitative DNA extraction, hydrophilic ionic liquids (ILs) are highly beneficial. This DNA extraction procedure is affordable and takes only a few minutes to complete. Ionic liquids can dissolve biomass and release DNA molecules from tissues and cells. DNA released in the supernatant may be used as such for downstream applications. The extraction procedure includes mixing ionic liquids with starting sample at a high temperature (65–95 °C), depending upon the sample source. If the plant or animal tissues are being used, brief centrifugation may be needed. Aqueous supernatant may be directly used for downstream applications. This approach is suited for high sample throughput and allows DNA extraction from bacteria, plant, and animal tissues in resource-constrained environments. Therefore, this method is most suitable for the detection of contaminations in food. The major disadvantage of this approach is that it might result in highly diluted DNA, which requires subsequent concentration by using DNA-binding columns or silica-coated magnetic beads." @default.
- W4386652456 created "2023-09-13" @default.
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- W4386652456 date "2022-01-01" @default.
- W4386652456 modified "2023-10-18" @default.
- W4386652456 title "DNA Isolation by Hydrophilic Ionic Liquid Treatment" @default.
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- W4386652456 doi "https://doi.org/10.1007/978-3-030-94230-4_7" @default.
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