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- W43866941 abstract "This chapter describes the methods for the separation and characterization of subpopulations of T cells. In normal adult lymphoid tissue, T lymphocytes represent a morphologically homogeneous population of small, round, and nondividing cells with a regularly shaped nucleus, little cytoplasm, and a few distinguishable specialized organelles. Physical separation methods have been successful in separating B and T lymphocytes on the basis of differences in sedimentation velocity, buoyant density, and electrophoretic mobility. One important application of physical separation methods to the study of T lymphocytes is the special case of lymphocyte activation. When stimulated by antigens, T lymphocytes undergo a complicated series of events that culminate in cell enlargement and proliferation. Activated T cells can be readily distinguished from their resting counterparts by velocity sedimentation or density gradient centrifugation analysis. Furthermore, such size differences can be utilized to follow the differentiation pathway of T lymphocytes that are repeatedly stimulated by cyclic exposure to antigens." @default.
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- W43866941 date "1987-01-01" @default.
- W43866941 modified "2023-10-18" @default.
- W43866941 title "Separation and Characterization of Subpopulations of T Cells" @default.
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- W43866941 doi "https://doi.org/10.1016/b978-0-12-564505-8.50010-1" @default.
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