FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4386698523> ?p ?o ?g. }

• W4386698523 abstract "Introduction Brown adipose tissue (BAT) dissipates energy in the form of heat majorly via the mitochondrial uncoupling protein 1 (UCP1). The activation of BAT, which is enriched in the neck area and contains brown and beige adipocytes in humans, was considered as a potential therapeutic target to treat obesity. Therefore, finding novel agents that can stimulate the differentiation and recruitment of brown or beige thermogenic adipocytes are important subjects for investigation. The current study investigated how the availability of extracellular thiamine (vitamin B1), an essential cofactor of mitochondrial enzyme complexes that catalyze key steps in the catabolism of nutrients, affects the expression of thermogenic marker genes and proteins and subsequent functional parameters during ex vivo adipocyte differentiation. Methods We differentiated primary human adipogenic progenitors that were cultivated from subcutaneous (SC) or deep neck (DN) adipose tissues in the presence of gradually increasing thiamine concentrations during their 14-day differentiation program. mRNA and protein expression of thermogenic genes were analyzed by RT-qPCR and western blot, respectively. Cellular respiration including stimulated maximal and proton-leak respiration was measured by Seahorse analysis. Results Higher thiamine levels resulted in increased expression of thiamine transporter 1 and 2 both at mRNA and protein levels in human neck area-derived adipocytes. Gradually increasing concentrations of thiamine led to increased basal, cAMP-stimulated, and proton-leak respiration along with elevated mitochondrial biogenesis of the differentiated adipocytes. The extracellular thiamine availability during adipogenesis determined the expression levels of UCP1, PGC1a, CKMT2, and other browning-related genes and proteins in primary SC and DN-derived adipocytes in a concentration-dependent manner. Providing abundant amounts of thiamine further increased the thermogenic competency of the adipocytes. Discussion Case studies in humans reported that thiamine deficiency was found in patients with type 2 diabetes and obesity. Our study raises the possibility of a novel strategy with long-term thiamine supplementation, which can enhance the thermogenic competency of differentiating neck area-derived adipocytes for preventing or combating obesity." @default.
• W4386698523 created "2023-09-14" @default.
• W4386698523 creator A5014742619 @default.
• W4386698523 creator A5042736560 @default.
• W4386698523 creator A5050239628 @default.
• W4386698523 creator A5061320307 @default.
• W4386698523 creator A5081752332 @default.
• W4386698523 creator A5088305940 @default.
• W4386698523 date "2023-09-13" @default.
• W4386698523 modified "2023-10-14" @default.
• W4386698523 title "Extracellular thiamine concentration influences thermogenic competency of differentiating neck area-derived human adipocytes" @default.
• W4386698523 cites W1583300816 @default.
• W4386698523 cites W1595439970 @default.
• W4386698523 cites W1618991848 @default.
• W4386698523 cites W1843349889 @default.
• W4386698523 cites W1965079305 @default.
• W4386698523 cites W1968517618 @default.
• W4386698523 cites W1974058241 @default.
• W4386698523 cites W1975084979 @default.
• W4386698523 cites W1979010935 @default.
• W4386698523 cites W1982578173 @default.
• W4386698523 cites W1994029836 @default.
• W4386698523 cites W2000037643 @default.
• W4386698523 cites W2002499203 @default.
• W4386698523 cites W2020585948 @default.
• W4386698523 cites W2027753197 @default.
• W4386698523 cites W2039005156 @default.
• W4386698523 cites W2040328785 @default.
• W4386698523 cites W2049912535 @default.
• W4386698523 cites W2090979867 @default.
• W4386698523 cites W2095010452 @default.
• W4386698523 cites W2096272680 @default.
• W4386698523 cites W2109257202 @default.
• W4386698523 cites W2111517662 @default.
• W4386698523 cites W2127712914 @default.
• W4386698523 cites W2129326001 @default.
• W4386698523 cites W2132358997 @default.
• W4386698523 cites W2147907319 @default.
• W4386698523 cites W2153276315 @default.
• W4386698523 cites W2170874580 @default.
• W4386698523 cites W21808250 @default.
• W4386698523 cites W2297285928 @default.
• W4386698523 cites W2558160402 @default.
• W4386698523 cites W2563599017 @default.
• W4386698523 cites W2736913806 @default.
• W4386698523 cites W2784374391 @default.
• W4386698523 cites W2784535312 @default.
• W4386698523 cites W2807414198 @default.
• W4386698523 cites W2889058844 @default.
• W4386698523 cites W2897657568 @default.
• W4386698523 cites W2901504087 @default.
• W4386698523 cites W2917027985 @default.
• W4386698523 cites W2938595851 @default.
• W4386698523 cites W2992922977 @default.
• W4386698523 cites W3017230513 @default.
• W4386698523 cites W3029176268 @default.
• W4386698523 cites W3046730026 @default.
• W4386698523 cites W3084210226 @default.
• W4386698523 cites W3120744005 @default.
• W4386698523 cites W3127128048 @default.
• W4386698523 cites W3137102179 @default.
• W4386698523 cites W3174737693 @default.
• W4386698523 cites W3194280519 @default.
• W4386698523 cites W3196191852 @default.
• W4386698523 cites W4206075841 @default.
• W4386698523 cites W4211140078 @default.
• W4386698523 cites W4285719527 @default.
• W4386698523 cites W4377690827 @default.
• W4386698523 doi "https://doi.org/10.3389/fnut.2023.1207394" @default.
• W4386698523 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/37781121" @default.
• W4386698523 hasPublicationYear "2023" @default.
• W4386698523 type Work @default.
• W4386698523 citedByCount "0" @default.
• W4386698523 crossrefType "journal-article" @default.
• W4386698523 hasAuthorship W4386698523A5014742619 @default.
• W4386698523 hasAuthorship W4386698523A5042736560 @default.
• W4386698523 hasAuthorship W4386698523A5050239628 @default.
• W4386698523 hasAuthorship W4386698523A5061320307 @default.
• W4386698523 hasAuthorship W4386698523A5081752332 @default.
• W4386698523 hasAuthorship W4386698523A5088305940 @default.
• W4386698523 hasBestOaLocation W43866985231 @default.
• W4386698523 hasConcept C122927707 @default.
• W4386698523 hasConcept C126322002 @default.
• W4386698523 hasConcept C134018914 @default.
• W4386698523 hasConcept C151279926 @default.
• W4386698523 hasConcept C171089720 @default.
• W4386698523 hasConcept C185592680 @default.
• W4386698523 hasConcept C2776203871 @default.
• W4386698523 hasConcept C28406088 @default.
• W4386698523 hasConcept C55493867 @default.
• W4386698523 hasConcept C71924100 @default.
• W4386698523 hasConcept C81979416 @default.
• W4386698523 hasConcept C86803240 @default.
• W4386698523 hasConcept C95444343 @default.
• W4386698523 hasConceptScore W4386698523C122927707 @default.
• W4386698523 hasConceptScore W4386698523C126322002 @default.
• W4386698523 hasConceptScore W4386698523C134018914 @default.
• W4386698523 hasConceptScore W4386698523C151279926 @default.
• W4386698523 hasConceptScore W4386698523C171089720 @default.
• W4386698523 hasConceptScore W4386698523C185592680 @default.

• next