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• W4386701025 endingPage "147736" @default.
• W4386701025 startingPage "147736" @default.
• W4386701025 abstract "The current study aimed to evaluate the genetic diversity, level of admixture, and phylogenetic relationship, of the Pakistani horse breeds, along with their morphological characterization. Data for the body measurements showed that Morna horses had the highest values of body height, body length, chest girth, leg length, and head length, whereas the Baluchi horses had the lowest values for these traits. For the genetic diversity 64 animals, 15/breed except for Baluchi(14) and Topras(05), were genotyped by using the 17-plex equine genotyping kit. The AMOVA results showed that 13% of genetic diversity was explained by breed differences, whereas 27% and 60% came from among and within individuals respectively. The highest values of genetic diversity parameters including Na(7.29±0.29), Ne(5.73±0.28), Ho(0.74±0.05) and He(0.82±0.01) were observed for Morna, whereas their lowest values were found for Topras. However, the highest value of inbreeding coefficient (Fis) was found for Baluchi and the lowest for KB horses. Among the markers, CA425 was found as the most polymorphic and ASB23 as the least polymorphic and highly fixed marker. Results of structure analysis revealed that, except Topras, each local horse breed had very admixed genetic structure perhaps due to their continuous crossing with other breeds in the past. Moreover, the structure analysis also showed that Morna and Shiean breeds had mixing of each other which was also confirmed by the lowest value of their pairwise Fst values and likewise the phylogenetic analysis also showed their close genetic relationship with each other. The phylogenetic analysis also revealed that Shiean breed had close genetic relationship with Arabian horses. Collectively, our data showed that Morna is the largest and genetically most diverse horse breed of Pakistan, whereas Baluchi horses are the smallest in size and have the highest values of inbreeding coefficient. And the phylogeny analysis showed that Shiean breed had close genetic relationship with the Arabian." @default.
• W4386701025 created "2023-09-14" @default.
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• W4386701025 date "2023-12-01" @default.
• W4386701025 modified "2023-10-16" @default.
• W4386701025 title "Unravelling of the genetic diversity, population structure, and phylogenetic relationships of the indigenous horse breeds of Pakistan" @default.
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• W4386701025 doi "https://doi.org/10.1016/j.gene.2023.147736" @default.
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