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- W4386773896 abstract "Viral transsynaptic labeling has become indispensable for investigating the functional connectivity of neural circuits in the mammalian brain. Adeno-associated virus serotype 1 (AAV1) allows for anterograde transneuronal labeling and manipulation of postsynaptic neurons. However, it is limited to delivering an AAV1 expressing a recombinase which relies on using transgenic animals or genetic access to postsynaptic neurons. We reasoned that a strong expression level could overcome this limitation. To this end, we used a self-complementary AAV of serotype 1 (scAAV1) under a strong promoter (CAG). We demonstrated the anterograde transneuronal efficiency of scAAV1 by delivering a fluorescent marker in mouse retina-superior colliculus and thalamic-amygdala pathways in a recombinase-independent manner in the mouse brain. In addition to investigating neuronal connectivity, anterograde transsynaptic AAVs with a strong promoter may be suitable for functional mapping and imaging." @default.
- W4386773896 created "2023-09-16" @default.
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- W4386773896 date "2023-09-15" @default.
- W4386773896 modified "2023-10-17" @default.
- W4386773896 title "Recombinase-independent AAV for anterograde transsynaptic tracing" @default.
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- W4386773896 doi "https://doi.org/10.1186/s13041-023-01053-7" @default.
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