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- W4386782351 abstract "Abstract Mechanical measurements of cells can provide unique insights into cell state and disease processes. The overall mechanical properties of cells can be heavily affected by the stiffest organelle, the nucleus. However, it is challenging to fully characterize internal nuclear structures in most cell mechanical measurement platforms. Here, we demonstrate single-cell deformability measurements of whole cells and stained nuclei in a fluorescence imaging flow cytometry platform. We also introduce bending energy derived metrics as a way to normalize measurements of cytoskeletal cortex and nuclear shape changes of cells and demonstrate the utility of relative deformability distributions to characterize populations of cells. We apply the platform to measure changes in cell biophysical properties during the process of NETosis, whereby neutrophils undergo drastic nuclear restructuring. We characterize cell size, deformability, and nuclear structure changes and their correlations in thousands of neutrophils undergoing NETosis, a process implicated in development of critical disease states, such as sepsis. This platform can aid in understanding heterogeneity in deformability in cell populations and how this may be influenced by nuclear or internal structure changes. Graphical Abstract" @default.
- W4386782351 created "2023-09-16" @default.
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- W4386782351 date "2023-09-15" @default.
- W4386782351 modified "2023-10-06" @default.
- W4386782351 title "Fluorescence imaging deformability cytometry: integrating nuclear structure with mechanical phenotyping" @default.
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- W4386782351 doi "https://doi.org/10.1007/s44258-023-00008-w" @default.
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