Matches in SemOpenAlex for { <https://semopenalex.org/work/W4386813312> ?p ?o ?g. }
- W4386813312 endingPage "107330" @default.
- W4386813312 startingPage "107330" @default.
- W4386813312 abstract "Glycerol is a cryoprotectant used widely for the cryopreservation of animal sperm, but it is linked to a decrease in fertility. The mechanism underlying the negative effects of glycerol remains unclear. Therefore, in this study, we aimed to gain a better understanding by using the chicken model. First, we investigated the impact of increasing the concentration of glycerol during insemination on hen fertility. Our findings revealed that 2% glycerol resulted in partial infertility, while 6% glycerol led to complete infertility. Subsequently, we examined the ability of sperm to colonize sperm storage tubules (SST) during in vivo insemination and in vitro incubation. The sperm used in the experiment were stained with Hoechst and contained 0, 2, or 6% glycerol. Furthermore, we conducted perivitelline membrane lysis tests and investigated sperm motility, mitochondrial function, ATP concentration, membrane integrity, and apoptosis after 60 min of incubation with different glycerol concentrations (0%, 1%, 2%, 6%, and 11%) at two temperatures to simulate pre-freezing (4 °C) and post-insemination (41 °C) conditions. Whereas 2% glycerol significantly reduced 50% of sperm containing SST, 6% glycerol completely inhibited SST colonization in vivo. On the other hand, in vitro incubation of sperm with SST revealed no effect of 2% glycerol, and 6% glycerol showed only a 17% reduction in sperm-filled SST. Moreover, glycerol reduced sperm-egg penetration rates and also affected sperm motility, bioenergetic metabolism, and cell death at 4 °C. These effects were observed when the concentration of glycerol exceeded 6%. Furthermore, at 41 °C, glycerol caused even greater damage, particularly in terms of reducing sperm motility. These data altogether reveal important effects of glycerol on sperm biology, sperm migration, SST colonization, and oocyte penetration. This suggests that glycerol plays a role in reducing fertility and presents opportunities for improving sperm cryopreservation." @default.
- W4386813312 created "2023-09-18" @default.
- W4386813312 creator A5000080590 @default.
- W4386813312 creator A5012407514 @default.
- W4386813312 creator A5014228465 @default.
- W4386813312 creator A5037258734 @default.
- W4386813312 creator A5038587089 @default.
- W4386813312 creator A5045769885 @default.
- W4386813312 creator A5054953649 @default.
- W4386813312 creator A5058189405 @default.
- W4386813312 creator A5085034978 @default.
- W4386813312 date "2023-11-01" @default.
- W4386813312 modified "2023-10-09" @default.
- W4386813312 title "Is Glycerol a Good Cryoprotectant for Sperm Cells? New Exploration of its Toxicity Using Avian Model" @default.
- W4386813312 cites W1893838320 @default.
- W4386813312 cites W1957534126 @default.
- W4386813312 cites W1975642414 @default.
- W4386813312 cites W1975731421 @default.
- W4386813312 cites W1982388589 @default.
- W4386813312 cites W1984080978 @default.
- W4386813312 cites W1987603756 @default.
- W4386813312 cites W1992370691 @default.
- W4386813312 cites W1994940442 @default.
- W4386813312 cites W1998443729 @default.
- W4386813312 cites W2000507065 @default.
- W4386813312 cites W2000578871 @default.
- W4386813312 cites W2022541258 @default.
- W4386813312 cites W2026488667 @default.
- W4386813312 cites W2028795580 @default.
- W4386813312 cites W2039548557 @default.
- W4386813312 cites W2043420622 @default.
- W4386813312 cites W2043879177 @default.
- W4386813312 cites W2059384421 @default.
- W4386813312 cites W2073758325 @default.
- W4386813312 cites W2074435782 @default.
- W4386813312 cites W2084076094 @default.
- W4386813312 cites W2093783485 @default.
- W4386813312 cites W2106473409 @default.
- W4386813312 cites W2112477651 @default.
- W4386813312 cites W2116596986 @default.
- W4386813312 cites W2118282874 @default.
- W4386813312 cites W2119116563 @default.
- W4386813312 cites W2119683728 @default.
- W4386813312 cites W2119915057 @default.
- W4386813312 cites W2122801031 @default.
- W4386813312 cites W2124448148 @default.
- W4386813312 cites W2127882934 @default.
- W4386813312 cites W2131814024 @default.
- W4386813312 cites W2139876142 @default.
- W4386813312 cites W2140265393 @default.
- W4386813312 cites W2162690227 @default.
- W4386813312 cites W2164449257 @default.
- W4386813312 cites W2171025589 @default.
- W4386813312 cites W2516927941 @default.
- W4386813312 cites W2605987559 @default.
- W4386813312 cites W2739647137 @default.
- W4386813312 cites W2810304164 @default.
- W4386813312 cites W2965352872 @default.
- W4386813312 cites W2978526191 @default.
- W4386813312 cites W2985394097 @default.
- W4386813312 cites W2996076422 @default.
- W4386813312 cites W3003693506 @default.
- W4386813312 cites W3011361605 @default.
- W4386813312 cites W3032227625 @default.
- W4386813312 cites W3038361694 @default.
- W4386813312 cites W3091755319 @default.
- W4386813312 cites W3098190194 @default.
- W4386813312 cites W3123363299 @default.
- W4386813312 cites W3127937138 @default.
- W4386813312 cites W3132703167 @default.
- W4386813312 cites W3155093312 @default.
- W4386813312 cites W3165426896 @default.
- W4386813312 cites W3214864303 @default.
- W4386813312 cites W4205849071 @default.
- W4386813312 cites W4220845044 @default.
- W4386813312 cites W4221125455 @default.
- W4386813312 cites W4224318497 @default.
- W4386813312 cites W4283270915 @default.
- W4386813312 cites W4283742304 @default.
- W4386813312 doi "https://doi.org/10.1016/j.anireprosci.2023.107330" @default.
- W4386813312 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/37734123" @default.
- W4386813312 hasPublicationYear "2023" @default.
- W4386813312 type Work @default.
- W4386813312 citedByCount "0" @default.
- W4386813312 crossrefType "journal-article" @default.
- W4386813312 hasAuthorship W4386813312A5000080590 @default.
- W4386813312 hasAuthorship W4386813312A5012407514 @default.
- W4386813312 hasAuthorship W4386813312A5014228465 @default.
- W4386813312 hasAuthorship W4386813312A5037258734 @default.
- W4386813312 hasAuthorship W4386813312A5038587089 @default.
- W4386813312 hasAuthorship W4386813312A5045769885 @default.
- W4386813312 hasAuthorship W4386813312A5054953649 @default.
- W4386813312 hasAuthorship W4386813312A5058189405 @default.
- W4386813312 hasAuthorship W4386813312A5085034978 @default.
- W4386813312 hasConcept C16685009 @default.
- W4386813312 hasConcept C179933525 @default.
- W4386813312 hasConcept C185592680 @default.
- W4386813312 hasConcept C196843134 @default.