Matches in SemOpenAlex for { <https://semopenalex.org/work/W4386814614> ?p ?o ?g. }
- W4386814614 endingPage "1942" @default.
- W4386814614 startingPage "1942" @default.
- W4386814614 abstract "Equine sarcoids (EqS) are fibroblast-derived skin tumors associated with bovine papillomavirus 1 and 2 (BPV-1 and -2). Based on Southern blotting, the BPV-1 genome was not found to be integrated in the host cell genome, suggesting that EqS pathogenesis does not result from insertional mutagenesis. Hence, CRISPR/Cas9 implies an interesting tool for selectively targeting BPV-1 episomes or genetically anchored suspected host factors. To address this in a proof-of-concept study, we confirmed the exclusive episomal persistence of BPV-1 in EqS using targeted locus amplification (TLA). To investigate the CRISPR/Cas9-mediated editing of BPV-1 episomes, primary equine fibroblast cultures were established and characterized. In the EqS fibroblast cultures, CRISPR-mediated targeting of the episomal E5 and E6 oncogenes as well as the BPV-1 long control region was successful and resulted in a pronounced reduction of the BPV-1 load. Moreover, the deletion of the equine Vimentin (VIM), which is highly expressed in EqS, considerably decreased the number of BPV-1 episomes. Our results suggest CRISPR/Cas9-based gene targeting may serve as a tool to help further unravel the biology of EqS pathogenesis." @default.
- W4386814614 created "2023-09-18" @default.
- W4386814614 creator A5004745283 @default.
- W4386814614 creator A5005813637 @default.
- W4386814614 creator A5017173720 @default.
- W4386814614 creator A5041935538 @default.
- W4386814614 creator A5045807702 @default.
- W4386814614 creator A5054147563 @default.
- W4386814614 creator A5058760199 @default.
- W4386814614 creator A5062133190 @default.
- W4386814614 creator A5072667867 @default.
- W4386814614 date "2023-09-17" @default.
- W4386814614 modified "2023-09-30" @default.
- W4386814614 title "CRISPR/Cas9-Mediated Targeting of BPV-1-Transformed Primary Equine Sarcoid Fibroblasts" @default.
- W4386814614 cites W1511843516 @default.
- W4386814614 cites W1569876593 @default.
- W4386814614 cites W1853713129 @default.
- W4386814614 cites W1895786380 @default.
- W4386814614 cites W1976935897 @default.
- W4386814614 cites W1979954728 @default.
- W4386814614 cites W1983870011 @default.
- W4386814614 cites W198819343 @default.
- W4386814614 cites W2001659704 @default.
- W4386814614 cites W2014331589 @default.
- W4386814614 cites W2022324744 @default.
- W4386814614 cites W2025050750 @default.
- W4386814614 cites W2040967677 @default.
- W4386814614 cites W2053542738 @default.
- W4386814614 cites W2070842927 @default.
- W4386814614 cites W2087064593 @default.
- W4386814614 cites W2089963048 @default.
- W4386814614 cites W2090173726 @default.
- W4386814614 cites W2096183242 @default.
- W4386814614 cites W2099259774 @default.
- W4386814614 cites W2106070783 @default.
- W4386814614 cites W2106637247 @default.
- W4386814614 cites W2119993175 @default.
- W4386814614 cites W2128237281 @default.
- W4386814614 cites W2132940890 @default.
- W4386814614 cites W2139780320 @default.
- W4386814614 cites W2167279371 @default.
- W4386814614 cites W2169150541 @default.
- W4386814614 cites W2267418770 @default.
- W4386814614 cites W2283271823 @default.
- W4386814614 cites W2325113387 @default.
- W4386814614 cites W2328400633 @default.
- W4386814614 cites W2409508105 @default.
- W4386814614 cites W2546270691 @default.
- W4386814614 cites W2572887968 @default.
- W4386814614 cites W2586267950 @default.
- W4386814614 cites W2611739609 @default.
- W4386814614 cites W2769957928 @default.
- W4386814614 cites W2944957886 @default.
- W4386814614 cites W3000307676 @default.
- W4386814614 cites W3005400930 @default.
- W4386814614 cites W3041289578 @default.
- W4386814614 cites W3080690697 @default.
- W4386814614 cites W3152289621 @default.
- W4386814614 cites W3182965326 @default.
- W4386814614 cites W3212374509 @default.
- W4386814614 cites W4206987113 @default.
- W4386814614 cites W4253188015 @default.
- W4386814614 cites W4381056890 @default.
- W4386814614 doi "https://doi.org/10.3390/v15091942" @default.
- W4386814614 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/37766348" @default.
- W4386814614 hasPublicationYear "2023" @default.
- W4386814614 type Work @default.
- W4386814614 citedByCount "0" @default.
- W4386814614 crossrefType "journal-article" @default.
- W4386814614 hasAuthorship W4386814614A5004745283 @default.
- W4386814614 hasAuthorship W4386814614A5005813637 @default.
- W4386814614 hasAuthorship W4386814614A5017173720 @default.
- W4386814614 hasAuthorship W4386814614A5041935538 @default.
- W4386814614 hasAuthorship W4386814614A5045807702 @default.
- W4386814614 hasAuthorship W4386814614A5054147563 @default.
- W4386814614 hasAuthorship W4386814614A5058760199 @default.
- W4386814614 hasAuthorship W4386814614A5062133190 @default.
- W4386814614 hasAuthorship W4386814614A5072667867 @default.
- W4386814614 hasBestOaLocation W43868146141 @default.
- W4386814614 hasConcept C104317684 @default.
- W4386814614 hasConcept C107635520 @default.
- W4386814614 hasConcept C132455925 @default.
- W4386814614 hasConcept C141231307 @default.
- W4386814614 hasConcept C144501496 @default.
- W4386814614 hasConcept C153911025 @default.
- W4386814614 hasConcept C16318435 @default.
- W4386814614 hasConcept C22744801 @default.
- W4386814614 hasConcept C2780381497 @default.
- W4386814614 hasConcept C501734568 @default.
- W4386814614 hasConcept C54355233 @default.
- W4386814614 hasConcept C81885089 @default.
- W4386814614 hasConcept C84597430 @default.
- W4386814614 hasConcept C86803240 @default.
- W4386814614 hasConcept C98108389 @default.
- W4386814614 hasConceptScore W4386814614C104317684 @default.
- W4386814614 hasConceptScore W4386814614C107635520 @default.
- W4386814614 hasConceptScore W4386814614C132455925 @default.
- W4386814614 hasConceptScore W4386814614C141231307 @default.