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• W4386815577 abstract "Transcription is the primary regulatory step in gene expression. Divergent transcription initiation from promoters and enhancers produces stable RNAs from genes and unstable RNAs from enhancers 1-5 . Nascent RNA capture and sequencing assays simultaneously measure gene and enhancer activity in cell populations 6-9 . However, fundamental questions in the temporal regulation of transcription and enhancer-gene synchrony remain unanswered primarily due to the absence of a single-cell perspective on active transcription. In this study, we present scGRO-seq - a novel single-cell nascent RNA sequencing assay using click-chemistry - and unveil the coordinated transcription throughout the genome. scGRO-seq demonstrates the episodic nature of transcription, and estimates burst size and frequency by directly quantifying transcribing RNA polymerases in individual cells. It reveals the co-transcription of functionally related genes and leverages the replication-dependent non-polyadenylated histone genes transcription to elucidate cell-cycle dynamics. The single-nucleotide spatial and temporal resolution of scGRO-seq identifies networks of enhancers and genes and indicates that the bursting of transcription at super-enhancers precedes the burst from associated genes. By imparting insights into the dynamic nature of transcription and the origin and propagation of transcription signals, scGRO-seq demonstrates its unique ability to investigate the mechanisms of transcription regulation and the role of enhancers in gene expression." @default.
• W4386815577 created "2023-09-18" @default.
• W4386815577 creator A5001813174 @default.
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• W4386815577 date "2023-09-16" @default.
• W4386815577 modified "2023-10-17" @default.
• W4386815577 title "Single-cell nascent RNA sequencing using click-chemistry unveils coordinated transcription" @default.
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• W4386815577 doi "https://doi.org/10.1101/2023.09.15.558015" @default.
• W4386815577 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/37745427" @default.
• W4386815577 hasPublicationYear "2023" @default.
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