FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4386816034> ?p ?o ?g. }

• W4386816034 abstract "Abstract Circulating Tumor Cells (CTCs) are commonly analyzed through genomic profiling, which does not capture posttranslational and functional alterations of encoded proteins. To address this limitation, we developed ZeptoCTC, a single-cell protein analysis workflow that combines established technologies for single-cell isolation and sensitive Reverse Phase Protein Array (RPPA) analysis to assess multiple protein expression and activation in individual CTCs. The workflow involves single cell labeling, isolation, lysis, and printing of the true single cell lysates onto a ZeptoChip using a modified micromanipulator CellCelector TM . Subsequently, the printed lysates undergo fluorescence immunoassay RPPA protein detection using a ZeptoReader followed by signal quantification with Image J software. ZeptoCTC was successfully optimized, beginning with the measurement of EpCAM protein expression—a standard marker for CTC detection. As expected, mean fluorescence signals for EpCAM levels were significantly higher in single MCF-7 cells compared to MDA-MB-231 cells. Next, Capivasertib-treated MCF-7 cells exhibited an approximately 2-fold increase in the pAkt/Akt ratio compared to non-treated control cells. This finding was consistent with a co-performed western blot analysis of pooled MCF-7 cells. Application of ZeptoCTC to the analysis of single CTCs derived from a metastasized breast cancer (MBC) patient indicated a significantly higher level of pAkt, accompanied by a corresponding increase in pErk level when compared to patient-matched WBC. Finally, the current workflow successfully indicated the detectable pAkt and Akt signal difference in CTCs from two MBC patients: one with an Akt1 wild-type genotype, and the other harboring approximately 80% Akt1(E17K) mutated CTCs. The mutated CTCs revealed clearly elevated pAkt levels (1.8-fold), along with an even more strongly elevated total Akt (3.4-fold) when compared to the respective signals measured in wild-type CTCs. In conclusion, ZeptoCTC is a highly sensitive method for measuring the expression and phosphorylation of treatment-relevant proteins in key cancer-driving signaling pathways from true single cell samples." @default.
• W4386816034 created "2023-09-18" @default.
• W4386816034 creator A5008793420 @default.
• W4386816034 creator A5011069826 @default.
• W4386816034 creator A5012063650 @default.
• W4386816034 creator A5013193544 @default.
• W4386816034 creator A5025192815 @default.
• W4386816034 creator A5031108943 @default.
• W4386816034 creator A5038085887 @default.
• W4386816034 creator A5051816392 @default.
• W4386816034 creator A5064045176 @default.
• W4386816034 creator A5077674559 @default.
• W4386816034 creator A5080385779 @default.
• W4386816034 creator A5086791040 @default.
• W4386816034 date "2023-09-16" @default.
• W4386816034 modified "2023-09-27" @default.
• W4386816034 title "ZeptoCTC - Sensitive Protein Analysis of True Single Cell Lysates using Reverse Phase Protein Arrays (RPPA)" @default.
• W4386816034 cites W1772652938 @default.
• W4386816034 cites W1991166678 @default.
• W4386816034 cites W2004524502 @default.
• W4386816034 cites W2018023891 @default.
• W4386816034 cites W2061078277 @default.
• W4386816034 cites W2071665937 @default.
• W4386816034 cites W2075754351 @default.
• W4386816034 cites W2102005789 @default.
• W4386816034 cites W2120018526 @default.
• W4386816034 cites W2135784953 @default.
• W4386816034 cites W2144040817 @default.
• W4386816034 cites W2148782878 @default.
• W4386816034 cites W2183855403 @default.
• W4386816034 cites W2340761255 @default.
• W4386816034 cites W2502148278 @default.
• W4386816034 cites W2562523657 @default.
• W4386816034 cites W2587480645 @default.
• W4386816034 cites W2598882867 @default.
• W4386816034 cites W2807662170 @default.
• W4386816034 cites W2903954148 @default.
• W4386816034 cites W2914923744 @default.
• W4386816034 cites W2919779091 @default.
• W4386816034 cites W2951821937 @default.
• W4386816034 cites W2981974127 @default.
• W4386816034 cites W2989306592 @default.
• W4386816034 cites W2992698875 @default.
• W4386816034 cites W2994836628 @default.
• W4386816034 cites W3024604435 @default.
• W4386816034 cites W3041326933 @default.
• W4386816034 cites W3060060316 @default.
• W4386816034 cites W3109793670 @default.
• W4386816034 cites W3115202206 @default.
• W4386816034 cites W3134218627 @default.
• W4386816034 cites W3134897856 @default.
• W4386816034 cites W3135446690 @default.
• W4386816034 cites W3159931666 @default.
• W4386816034 cites W3169325545 @default.
• W4386816034 cites W3208924486 @default.
• W4386816034 cites W3215991548 @default.
• W4386816034 cites W3217081290 @default.
• W4386816034 cites W4200080267 @default.
• W4386816034 cites W4200492909 @default.
• W4386816034 cites W4206015960 @default.
• W4386816034 cites W4210417953 @default.
• W4386816034 cites W4210483916 @default.
• W4386816034 cites W4213421391 @default.
• W4386816034 cites W4307163279 @default.
• W4386816034 cites W4321607941 @default.
• W4386816034 cites W573909598 @default.
• W4386816034 doi "https://doi.org/10.1101/2023.09.16.558042" @default.
• W4386816034 hasPublicationYear "2023" @default.
• W4386816034 type Work @default.
• W4386816034 citedByCount "0" @default.
• W4386816034 crossrefType "posted-content" @default.
• W4386816034 hasAuthorship W4386816034A5008793420 @default.
• W4386816034 hasAuthorship W4386816034A5011069826 @default.
• W4386816034 hasAuthorship W4386816034A5012063650 @default.
• W4386816034 hasAuthorship W4386816034A5013193544 @default.
• W4386816034 hasAuthorship W4386816034A5025192815 @default.
• W4386816034 hasAuthorship W4386816034A5031108943 @default.
• W4386816034 hasAuthorship W4386816034A5038085887 @default.
• W4386816034 hasAuthorship W4386816034A5051816392 @default.
• W4386816034 hasAuthorship W4386816034A5064045176 @default.
• W4386816034 hasAuthorship W4386816034A5077674559 @default.
• W4386816034 hasAuthorship W4386816034A5080385779 @default.
• W4386816034 hasAuthorship W4386816034A5086791040 @default.
• W4386816034 hasBestOaLocation W43868160341 @default.
• W4386816034 hasConcept C104317684 @default.
• W4386816034 hasConcept C11960822 @default.
• W4386816034 hasConcept C121608353 @default.
• W4386816034 hasConcept C1491633281 @default.
• W4386816034 hasConcept C153911025 @default.
• W4386816034 hasConcept C185592680 @default.
• W4386816034 hasConcept C2776415932 @default.
• W4386816034 hasConcept C2776950831 @default.
• W4386816034 hasConcept C2779013556 @default.
• W4386816034 hasConcept C502942594 @default.
• W4386816034 hasConcept C54355233 @default.
• W4386816034 hasConcept C55493867 @default.
• W4386816034 hasConcept C61238886 @default.
• W4386816034 hasConcept C75217442 @default.
• W4386816034 hasConcept C86803240 @default.
• W4386816034 hasConcept C95444343 @default.

• next