Matches in SemOpenAlex for { <https://semopenalex.org/work/W4386819673> ?p ?o ?g. }
- W4386819673 abstract "Abstract Fluidic habitats are very common to bacterial life, however, very little is known about the effect of the flow stresses on the virulence of the bacteria. In the present work, we conduct microfluidic experiments to understand the consequence of stresses generated by flowing fluid on the bacterial morphology and virulence. We consider Klebsiella pneumoniae ( KP ), an ESKAPE pathogen as the model bacteria that are responsible for blood stream infections like bacteremia apart from pneumonia, urinary tract infections and more. We generate four different stress conditions by changing the flow rate and channel geometry subsequently altering the shear rate and stressing time (τ). We observe significant changes in the structural aspects of the stressed bacteria. With an increase in stressing parameters, the viability of the bacterial sample deteriorated. Most importantly, these stressed samples proliferate much more than unstressed samples inside the RAW264.7 murine macrophages. The results shed light on the complex relationship between flow stresses and bacterial virulence. Furthermore, we challenge the bacterial samples with ciprofloxacin to see how they behave under different stress conditions. The present study can be extended to model deadly diseases like bacteremia using organ-on-a-chip technology and help understand bacterial pathogenicity under realistic environments. Figure: A schematic representation of the present work. Figure created with BioRender ( www.biorender.com )" @default.
- W4386819673 created "2023-09-19" @default.
- W4386819673 creator A5018333739 @default.
- W4386819673 creator A5030416693 @default.
- W4386819673 creator A5035974258 @default.
- W4386819673 creator A5046214220 @default.
- W4386819673 creator A5048912807 @default.
- W4386819673 creator A5068338535 @default.
- W4386819673 creator A5068504849 @default.
- W4386819673 date "2023-09-18" @default.
- W4386819673 modified "2023-09-29" @default.
- W4386819673 title "Klebsiella Pneumoniae turns more virulent under flow stresses in capillary like microchannels" @default.
- W4386819673 cites W1482673050 @default.
- W4386819673 cites W1531643733 @default.
- W4386819673 cites W1895017250 @default.
- W4386819673 cites W1977009598 @default.
- W4386819673 cites W1980232598 @default.
- W4386819673 cites W1983175129 @default.
- W4386819673 cites W1989440413 @default.
- W4386819673 cites W1990523202 @default.
- W4386819673 cites W1998301235 @default.
- W4386819673 cites W2001607419 @default.
- W4386819673 cites W2002534404 @default.
- W4386819673 cites W2004212428 @default.
- W4386819673 cites W2033117797 @default.
- W4386819673 cites W2035339894 @default.
- W4386819673 cites W2051257969 @default.
- W4386819673 cites W2058077403 @default.
- W4386819673 cites W2058507546 @default.
- W4386819673 cites W2060382006 @default.
- W4386819673 cites W2064441783 @default.
- W4386819673 cites W2072544855 @default.
- W4386819673 cites W2076922024 @default.
- W4386819673 cites W2079191152 @default.
- W4386819673 cites W2080090213 @default.
- W4386819673 cites W2084906731 @default.
- W4386819673 cites W2090665494 @default.
- W4386819673 cites W2123248530 @default.
- W4386819673 cites W2127299218 @default.
- W4386819673 cites W2140832306 @default.
- W4386819673 cites W2145069900 @default.
- W4386819673 cites W2152526860 @default.
- W4386819673 cites W2161157918 @default.
- W4386819673 cites W2162046649 @default.
- W4386819673 cites W2168769754 @default.
- W4386819673 cites W2176206796 @default.
- W4386819673 cites W2194016301 @default.
- W4386819673 cites W2214747519 @default.
- W4386819673 cites W2342539172 @default.
- W4386819673 cites W2480010979 @default.
- W4386819673 cites W2515497383 @default.
- W4386819673 cites W2528917045 @default.
- W4386819673 cites W2567912550 @default.
- W4386819673 cites W2614006786 @default.
- W4386819673 cites W2615232728 @default.
- W4386819673 cites W2615583214 @default.
- W4386819673 cites W2744332922 @default.
- W4386819673 cites W2804910112 @default.
- W4386819673 cites W2911281497 @default.
- W4386819673 cites W2913054963 @default.
- W4386819673 cites W2916337635 @default.
- W4386819673 cites W2934656438 @default.
- W4386819673 cites W2956217317 @default.
- W4386819673 cites W2999214061 @default.
- W4386819673 cites W2999304916 @default.
- W4386819673 cites W3024693033 @default.
- W4386819673 cites W3037222830 @default.
- W4386819673 cites W3099415337 @default.
- W4386819673 cites W3103012139 @default.
- W4386819673 cites W3135791115 @default.
- W4386819673 cites W3158045307 @default.
- W4386819673 cites W3162009173 @default.
- W4386819673 cites W3164574100 @default.
- W4386819673 cites W3166225254 @default.
- W4386819673 cites W3179489545 @default.
- W4386819673 cites W3180425518 @default.
- W4386819673 cites W4206677002 @default.
- W4386819673 cites W4214855071 @default.
- W4386819673 cites W4244780028 @default.
- W4386819673 cites W4253260323 @default.
- W4386819673 cites W4297154301 @default.
- W4386819673 cites W4324020393 @default.
- W4386819673 cites W4362717242 @default.
- W4386819673 doi "https://doi.org/10.1101/2023.09.18.558194" @default.
- W4386819673 hasPublicationYear "2023" @default.
- W4386819673 type Work @default.
- W4386819673 citedByCount "0" @default.
- W4386819673 crossrefType "posted-content" @default.
- W4386819673 hasAuthorship W4386819673A5018333739 @default.
- W4386819673 hasAuthorship W4386819673A5030416693 @default.
- W4386819673 hasAuthorship W4386819673A5035974258 @default.
- W4386819673 hasAuthorship W4386819673A5046214220 @default.
- W4386819673 hasAuthorship W4386819673A5048912807 @default.
- W4386819673 hasAuthorship W4386819673A5068338535 @default.
- W4386819673 hasAuthorship W4386819673A5068504849 @default.
- W4386819673 hasBestOaLocation W43868196731 @default.
- W4386819673 hasConcept C104317684 @default.
- W4386819673 hasConcept C171250308 @default.
- W4386819673 hasConcept C192562407 @default.
- W4386819673 hasConcept C2776460866 @default.